Development and Evaluation of a Rapid GII Norovirus Detection Method Based on CRISPR-Cas12a
Norovirus is highly infectious and rapidly transmissible and represents a major pathogen of sporadic cases and outbreaks of acute gastroenteritis worldwide, causing a substantial disease burden. Recent years have witnessed a dramatic increase in norovirus outbreaks in China, significantly higher tha...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Sciendo
2024-03-01
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| Series: | Polish Journal of Microbiology |
| Subjects: | |
| Online Access: | https://doi.org/10.33073/pjm-2024-009 |
| _version_ | 1827320899386212352 |
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| author | Hu Xinyi He Pei Jiang Tong Shen Jilu |
| author_facet | Hu Xinyi He Pei Jiang Tong Shen Jilu |
| author_sort | Hu Xinyi |
| collection | DOAJ |
| description | Norovirus is highly infectious and rapidly transmissible and represents a major pathogen of sporadic cases and outbreaks of acute gastroenteritis worldwide, causing a substantial disease burden. Recent years have witnessed a dramatic increase in norovirus outbreaks in China, significantly higher than in previous years, among which GII norovirus is the predominant prevalent strain. Therefore, rapid norovirus diagnosis is critical for clinical treatment and transmission control. Hence, we developed a molecular assay based on RPA combined with the CRISPER-CAS12a technique targeting the conserved region of the GII norovirus genome, the results of which could be displayed by fluorescence curves and immunochromatographic lateral-flow test strips. The reaction only required approximately 50 min, and the results were visible by the naked eye with a sensitivity reaching 102 copies/μl. Also, our method does not cross-react with other common pathogens that cause intestinal diarrhea. Furthermore, this assay was easy to perform and inexpensive, which could be widely applied for detecting norovirus in settings including medical institutions at all levels, particularly township health centers in low-resource areas. |
| first_indexed | 2024-04-25T00:55:14Z |
| format | Article |
| id | doaj.art-a7feca6d216d4a9eb6b93abb67118333 |
| institution | Directory Open Access Journal |
| issn | 2544-4646 |
| language | English |
| last_indexed | 2024-04-25T00:55:14Z |
| publishDate | 2024-03-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Polish Journal of Microbiology |
| spelling | doaj.art-a7feca6d216d4a9eb6b93abb671183332024-03-11T10:06:22ZengSciendoPolish Journal of Microbiology2544-46462024-03-01731899710.33073/pjm-2024-009Development and Evaluation of a Rapid GII Norovirus Detection Method Based on CRISPR-Cas12aHu Xinyi0He Pei1Jiang Tong2Shen Jilu31The First Affiliated Hospital of Anhui Medical University, Hefei, China1The First Affiliated Hospital of Anhui Medical University, Hefei, China1The First Affiliated Hospital of Anhui Medical University, Hefei, China1The First Affiliated Hospital of Anhui Medical University, Hefei, ChinaNorovirus is highly infectious and rapidly transmissible and represents a major pathogen of sporadic cases and outbreaks of acute gastroenteritis worldwide, causing a substantial disease burden. Recent years have witnessed a dramatic increase in norovirus outbreaks in China, significantly higher than in previous years, among which GII norovirus is the predominant prevalent strain. Therefore, rapid norovirus diagnosis is critical for clinical treatment and transmission control. Hence, we developed a molecular assay based on RPA combined with the CRISPER-CAS12a technique targeting the conserved region of the GII norovirus genome, the results of which could be displayed by fluorescence curves and immunochromatographic lateral-flow test strips. The reaction only required approximately 50 min, and the results were visible by the naked eye with a sensitivity reaching 102 copies/μl. Also, our method does not cross-react with other common pathogens that cause intestinal diarrhea. Furthermore, this assay was easy to perform and inexpensive, which could be widely applied for detecting norovirus in settings including medical institutions at all levels, particularly township health centers in low-resource areas.https://doi.org/10.33073/pjm-2024-009gii norovirusgastroenteritisrpacrispr-cas12adiagnostic |
| spellingShingle | Hu Xinyi He Pei Jiang Tong Shen Jilu Development and Evaluation of a Rapid GII Norovirus Detection Method Based on CRISPR-Cas12a Polish Journal of Microbiology gii norovirus gastroenteritis rpa crispr-cas12a diagnostic |
| title | Development and Evaluation of a Rapid GII Norovirus Detection Method Based on CRISPR-Cas12a |
| title_full | Development and Evaluation of a Rapid GII Norovirus Detection Method Based on CRISPR-Cas12a |
| title_fullStr | Development and Evaluation of a Rapid GII Norovirus Detection Method Based on CRISPR-Cas12a |
| title_full_unstemmed | Development and Evaluation of a Rapid GII Norovirus Detection Method Based on CRISPR-Cas12a |
| title_short | Development and Evaluation of a Rapid GII Norovirus Detection Method Based on CRISPR-Cas12a |
| title_sort | development and evaluation of a rapid gii norovirus detection method based on crispr cas12a |
| topic | gii norovirus gastroenteritis rpa crispr-cas12a diagnostic |
| url | https://doi.org/10.33073/pjm-2024-009 |
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