Histomorphometric and immunohistochemistry studies of the corpus luteum of bedouin goats reared in arid environment
The aim of this work was to study the histomorphometry and the immunohistochemistry of the Corpus luteum (CL) of Bedouin goat living in arid zones. Pregnant and non-pregnant females ovaries were collected from slaughterhouses, weighed, measured and then fixed in buffered formalin for histological, h...
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| Format: | Article |
| Language: | English |
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Kafkas University, Faculty of Veterinary Medicine
2023-06-01
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| Series: | Kafkas Universitesi Veteriner Fakültesi Dergisi |
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| Online Access: | https://vetdergikafkas.org/pdf.php?id=3002 |
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| author | Sara KASSOURI-MAOUCHE Nouria BOUKENAOUI-FERROUK Lynda AINOUZ-AMMAR AOUCHICHE Elara MOUDILOU Jean-Marie EXBRAYAT Farida KHAMMAR |
| author_facet | Sara KASSOURI-MAOUCHE Nouria BOUKENAOUI-FERROUK Lynda AINOUZ-AMMAR AOUCHICHE Elara MOUDILOU Jean-Marie EXBRAYAT Farida KHAMMAR |
| author_sort | Sara KASSOURI-MAOUCHE |
| collection | DOAJ |
| description | The aim of this work was to study the histomorphometry and the immunohistochemistry of the Corpus luteum (CL) of Bedouin
goat living in arid zones. Pregnant and non-pregnant females ovaries were collected from slaughterhouses, weighed, measured and then
fixed in buffered formalin for histological, histochemical and immunolocalization of Ki-67, active caspase-3, aromatase and progesterone
receptor. Our results showed that CL affected significantly the ovarian weight (P<0.001). The CL (P<0.05) and the large luteal cells
(P<0.001) diameters were higher in pregnant compared to the non-pregnant CL. In the non-pregnant CL, the immunostaining of Ki-67
was only observed in the small luteal cell"s nuclei whereas the active caspase-3 was detected in the large and small luteal cells cytoplasm.
The aromatase was also detected in the CL and capsule cells cytoplasm, in contrast the progesterone receptor was observed in all luteal
cell"s cytoplasm and in some luteal cell nuclei. We conclude that in the Bedouin goat, the CL affects the ovarian biometric parameters. The
development and luteolysis of non-pregnant CL seem to be under the balance between luteotropic and luteolytic markers, with luteolysis
occurring by apoptosis via the caspase-3 pathway. |
| first_indexed | 2024-03-12T10:23:55Z |
| format | Article |
| id | doaj.art-a7fed01cb6784a1796349d0cebdd4ccd |
| institution | Directory Open Access Journal |
| issn | 1309-2251 |
| language | English |
| last_indexed | 2024-03-12T10:23:55Z |
| publishDate | 2023-06-01 |
| publisher | Kafkas University, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| spelling | doaj.art-a7fed01cb6784a1796349d0cebdd4ccd2023-09-02T09:54:26ZengKafkas University, Faculty of Veterinary MedicineKafkas Universitesi Veteriner Fakültesi Dergisi1309-22512023-06-0129325526310.9775/kvfd.2023.291483002Histomorphometric and immunohistochemistry studies of the corpus luteum of bedouin goats reared in arid environmentSara KASSOURI-MAOUCHE0Nouria BOUKENAOUI-FERROUK1Lynda AINOUZ-AMMAR AOUCHICHE2Elara MOUDILOU3Jean-Marie EXBRAYAT4Farida KHAMMAR5Houari BOUMEDIENE University of Sciences and Technology, Faculty of Biology, Laboratory of Research on Arid Lands, Bab Ezzouar 16111, Algiers, ALGERIAHouari BOUMEDIENE University of Sciences and Technology, Faculty of Biology, Laboratory of Research on Arid Lands, Bab Ezzouar 16111, Algiers, ALGERIARabie BOUCHAMA National Higher Veterinary School, Oued Smar, 16059, Algiers, ALGERIAScience and Humanities Confluence Research Center - (EA 1598), 69002, Lyon, FRANCEScience and Humanities Confluence Research Center - (EA 1598), 69002, Lyon, FRANCEHouari BOUMEDIENE University of Sciences and Technology, Faculty of Biology, Laboratory of Research on Arid Lands, Bab Ezzouar 16111, Algiers, ALGERIAThe aim of this work was to study the histomorphometry and the immunohistochemistry of the Corpus luteum (CL) of Bedouin goat living in arid zones. Pregnant and non-pregnant females ovaries were collected from slaughterhouses, weighed, measured and then fixed in buffered formalin for histological, histochemical and immunolocalization of Ki-67, active caspase-3, aromatase and progesterone receptor. Our results showed that CL affected significantly the ovarian weight (P<0.001). The CL (P<0.05) and the large luteal cells (P<0.001) diameters were higher in pregnant compared to the non-pregnant CL. In the non-pregnant CL, the immunostaining of Ki-67 was only observed in the small luteal cell"s nuclei whereas the active caspase-3 was detected in the large and small luteal cells cytoplasm. The aromatase was also detected in the CL and capsule cells cytoplasm, in contrast the progesterone receptor was observed in all luteal cell"s cytoplasm and in some luteal cell nuclei. We conclude that in the Bedouin goat, the CL affects the ovarian biometric parameters. The development and luteolysis of non-pregnant CL seem to be under the balance between luteotropic and luteolytic markers, with luteolysis occurring by apoptosis via the caspase-3 pathway.https://vetdergikafkas.org/pdf.php?id=3002active caspase-3aromatasecorpus luteumgoathistomorphometryimmunohistochemistryprogesterone receptor |
| spellingShingle | Sara KASSOURI-MAOUCHE Nouria BOUKENAOUI-FERROUK Lynda AINOUZ-AMMAR AOUCHICHE Elara MOUDILOU Jean-Marie EXBRAYAT Farida KHAMMAR Histomorphometric and immunohistochemistry studies of the corpus luteum of bedouin goats reared in arid environment Kafkas Universitesi Veteriner Fakültesi Dergisi active caspase-3 aromatase corpus luteum goat histomorphometry immunohistochemistry progesterone receptor |
| title | Histomorphometric and immunohistochemistry studies of the corpus luteum of bedouin goats reared in arid environment |
| title_full | Histomorphometric and immunohistochemistry studies of the corpus luteum of bedouin goats reared in arid environment |
| title_fullStr | Histomorphometric and immunohistochemistry studies of the corpus luteum of bedouin goats reared in arid environment |
| title_full_unstemmed | Histomorphometric and immunohistochemistry studies of the corpus luteum of bedouin goats reared in arid environment |
| title_short | Histomorphometric and immunohistochemistry studies of the corpus luteum of bedouin goats reared in arid environment |
| title_sort | histomorphometric and immunohistochemistry studies of the corpus luteum of bedouin goats reared in arid environment |
| topic | active caspase-3 aromatase corpus luteum goat histomorphometry immunohistochemistry progesterone receptor |
| url | https://vetdergikafkas.org/pdf.php?id=3002 |
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