Neuron-specific knockouts indicate the importance of network communication to Drosophila rhythmicity
Animal circadian rhythms persist in constant darkness and are driven by intracellular transcription-translation feedback loops. Although these cellular oscillators communicate, isolated mammalian cellular clocks continue to tick away in darkness without intercellular communication. To investigate th...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2019-10-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/48301 |
| _version_ | 1828382802677071872 |
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| author | Matthias Schlichting Madelen M Díaz Jason Xin Michael Rosbash |
| author_facet | Matthias Schlichting Madelen M Díaz Jason Xin Michael Rosbash |
| author_sort | Matthias Schlichting |
| collection | DOAJ |
| description | Animal circadian rhythms persist in constant darkness and are driven by intracellular transcription-translation feedback loops. Although these cellular oscillators communicate, isolated mammalian cellular clocks continue to tick away in darkness without intercellular communication. To investigate these issues in Drosophila, we assayed behavior as well as molecular rhythms within individual brain clock neurons while blocking communication within the ca. 150 neuron clock network. We also generated CRISPR-mediated neuron-specific circadian clock knockouts. The results point to two key clock neuron groups: loss of the clock within both regions but neither one alone has a strong behavioral phenotype in darkness; communication between these regions also contributes to circadian period determination. Under these dark conditions, the clock within one region persists without network communication. The clock within the famous PDF-expressing s-LNv neurons however was strongly dependent on network communication, likely because clock gene expression within these vulnerable sLNvs depends on neuronal firing or light. |
| first_indexed | 2024-12-10T04:37:41Z |
| format | Article |
| id | doaj.art-a7ff6fa3445b4ed1b1d536be98cf21f4 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-12-10T04:37:41Z |
| publishDate | 2019-10-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-a7ff6fa3445b4ed1b1d536be98cf21f42022-12-22T02:01:57ZengeLife Sciences Publications LtdeLife2050-084X2019-10-01810.7554/eLife.48301Neuron-specific knockouts indicate the importance of network communication to Drosophila rhythmicityMatthias Schlichting0https://orcid.org/0000-0002-0822-0265Madelen M Díaz1Jason Xin2Michael Rosbash3https://orcid.org/0000-0003-3366-1780Department of Biology, Howard Hughes Medical Institute, Brandeis University, Waltham, United StatesDepartment of Biology, Howard Hughes Medical Institute, Brandeis University, Waltham, United StatesDepartment of Biology, Howard Hughes Medical Institute, Brandeis University, Waltham, United StatesDepartment of Biology, Howard Hughes Medical Institute, Brandeis University, Waltham, United StatesAnimal circadian rhythms persist in constant darkness and are driven by intracellular transcription-translation feedback loops. Although these cellular oscillators communicate, isolated mammalian cellular clocks continue to tick away in darkness without intercellular communication. To investigate these issues in Drosophila, we assayed behavior as well as molecular rhythms within individual brain clock neurons while blocking communication within the ca. 150 neuron clock network. We also generated CRISPR-mediated neuron-specific circadian clock knockouts. The results point to two key clock neuron groups: loss of the clock within both regions but neither one alone has a strong behavioral phenotype in darkness; communication between these regions also contributes to circadian period determination. Under these dark conditions, the clock within one region persists without network communication. The clock within the famous PDF-expressing s-LNv neurons however was strongly dependent on network communication, likely because clock gene expression within these vulnerable sLNvs depends on neuronal firing or light.https://elifesciences.org/articles/48301circadian rhythmnetwork communicationactivity |
| spellingShingle | Matthias Schlichting Madelen M Díaz Jason Xin Michael Rosbash Neuron-specific knockouts indicate the importance of network communication to Drosophila rhythmicity eLife circadian rhythm network communication activity |
| title | Neuron-specific knockouts indicate the importance of network communication to Drosophila rhythmicity |
| title_full | Neuron-specific knockouts indicate the importance of network communication to Drosophila rhythmicity |
| title_fullStr | Neuron-specific knockouts indicate the importance of network communication to Drosophila rhythmicity |
| title_full_unstemmed | Neuron-specific knockouts indicate the importance of network communication to Drosophila rhythmicity |
| title_short | Neuron-specific knockouts indicate the importance of network communication to Drosophila rhythmicity |
| title_sort | neuron specific knockouts indicate the importance of network communication to drosophila rhythmicity |
| topic | circadian rhythm network communication activity |
| url | https://elifesciences.org/articles/48301 |
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