Isolation and identification of oil sludge degrading bacteria from production tank Number 9 Masjed Soleiman
Introduction: “Bioremediation” is one of the most effective methods to remove petroleum contaminants. The aim of the present study is to isolate the indigenous bacteria from the waste petroleum in the Masjed Soleiman No. 9 production tank and to examine the effect of their application on the elimina...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
University of Isfahan
2014-07-01
|
| Series: | Biological Journal of Microorganism |
| Subjects: | |
| Online Access: | http://uijs.ui.ac.ir/bjm//browse.php?a_code=A-10-310-1&slc_lang=en&sid=1 |
| _version_ | 1818568904564277248 |
|---|---|
| author | Yalda Sheyni Hossein Motamedi Ahmadali Pourbabaei |
| author_facet | Yalda Sheyni Hossein Motamedi Ahmadali Pourbabaei |
| author_sort | Yalda Sheyni |
| collection | DOAJ |
| description | Introduction: “Bioremediation” is one of the most effective methods to remove petroleum contaminants. The aim of the present study is to isolate the indigenous bacteria from the waste petroleum in the Masjed Soleiman No. 9 production tank and to examine the effect of their application on the elimination of petroleum heavy chain hydrocarbons and converting them into light compounds . Materials and methods: Two percent of petroleum sludge was inoculated to the mineral basal medium and after proliferation of its indigenous bacteria, they were inoculated into the mixture of oil sludge and sand at level of 5%, and the amount of total hydrocarbons and residual oil were measured and compared. The isolates were identified based on biochemical tests and 16S rRNA gene sequencing. Optimization of nitrogen and phosphate sources was done based on growth curves of selected isolates. Gas chromatography was used to determine degradation of sludge hydrocarbons. Results: In this study, 10 bacterial isolates were isolated from petroleum sludge . Measurement of petroleum total hydrocarbons, using Soxhlet-extraction method, showed that two isolates named MIS1 and MIS2 are able to decompose oil sludge hydrocarbons within 7 days, with the yields of 62% and 72%, respectively. Furthermore, the two isolates reach the end of the logarithmic phase at 48 and 120 hrs, respectively. The best source of nitrogen and phosphate for both isolates was ammonium nitrate and potassium di hydrogen phosphate, respectively. The isolates were identified as Arthrobacter aurescens and Pseudomonas aeruginosa , respectively. In gas chromatography analysis it was revealed that Pseudomonas aeruginosa was more potent in degradation of heavy chain hydrocarbons and their conversion to light chain compounds. Discussion and conclusion: Resident bacteria are present in the oil sludge and are able to degrade the heavy petroleum compounds and convert them into light compounds. These bacteria are effective in the reduction of environmental pollution and production of low molecular weight hydrocarbons. |
| first_indexed | 2024-12-14T06:41:10Z |
| format | Article |
| id | doaj.art-a80352dceac4434fa952c0fdc3dfd0b9 |
| institution | Directory Open Access Journal |
| issn | 2322-5173 2322-5181 |
| language | English |
| last_indexed | 2024-12-14T06:41:10Z |
| publishDate | 2014-07-01 |
| publisher | University of Isfahan |
| record_format | Article |
| series | Biological Journal of Microorganism |
| spelling | doaj.art-a80352dceac4434fa952c0fdc3dfd0b92022-12-21T23:13:13ZengUniversity of IsfahanBiological Journal of Microorganism2322-51732322-51812014-07-013101326Isolation and identification of oil sludge degrading bacteria from production tank Number 9 Masjed SoleimanYalda Sheyni0Hossein Motamedi1Ahmadali Pourbabaei2 Islamic Avazd University- Qom branch Shahid Chamran University Islamic Avazd University- Qom branch Introduction: “Bioremediation” is one of the most effective methods to remove petroleum contaminants. The aim of the present study is to isolate the indigenous bacteria from the waste petroleum in the Masjed Soleiman No. 9 production tank and to examine the effect of their application on the elimination of petroleum heavy chain hydrocarbons and converting them into light compounds . Materials and methods: Two percent of petroleum sludge was inoculated to the mineral basal medium and after proliferation of its indigenous bacteria, they were inoculated into the mixture of oil sludge and sand at level of 5%, and the amount of total hydrocarbons and residual oil were measured and compared. The isolates were identified based on biochemical tests and 16S rRNA gene sequencing. Optimization of nitrogen and phosphate sources was done based on growth curves of selected isolates. Gas chromatography was used to determine degradation of sludge hydrocarbons. Results: In this study, 10 bacterial isolates were isolated from petroleum sludge . Measurement of petroleum total hydrocarbons, using Soxhlet-extraction method, showed that two isolates named MIS1 and MIS2 are able to decompose oil sludge hydrocarbons within 7 days, with the yields of 62% and 72%, respectively. Furthermore, the two isolates reach the end of the logarithmic phase at 48 and 120 hrs, respectively. The best source of nitrogen and phosphate for both isolates was ammonium nitrate and potassium di hydrogen phosphate, respectively. The isolates were identified as Arthrobacter aurescens and Pseudomonas aeruginosa , respectively. In gas chromatography analysis it was revealed that Pseudomonas aeruginosa was more potent in degradation of heavy chain hydrocarbons and their conversion to light chain compounds. Discussion and conclusion: Resident bacteria are present in the oil sludge and are able to degrade the heavy petroleum compounds and convert them into light compounds. These bacteria are effective in the reduction of environmental pollution and production of low molecular weight hydrocarbons.http://uijs.ui.ac.ir/bjm//browse.php?a_code=A-10-310-1&slc_lang=en&sid=1Bioremediation Oil sludge Total petroleum hydrocarbons Gas chromatography |
| spellingShingle | Yalda Sheyni Hossein Motamedi Ahmadali Pourbabaei Isolation and identification of oil sludge degrading bacteria from production tank Number 9 Masjed Soleiman Biological Journal of Microorganism Bioremediation Oil sludge Total petroleum hydrocarbons Gas chromatography |
| title | Isolation and identification of oil sludge degrading bacteria from production tank Number 9 Masjed Soleiman |
| title_full | Isolation and identification of oil sludge degrading bacteria from production tank Number 9 Masjed Soleiman |
| title_fullStr | Isolation and identification of oil sludge degrading bacteria from production tank Number 9 Masjed Soleiman |
| title_full_unstemmed | Isolation and identification of oil sludge degrading bacteria from production tank Number 9 Masjed Soleiman |
| title_short | Isolation and identification of oil sludge degrading bacteria from production tank Number 9 Masjed Soleiman |
| title_sort | isolation and identification of oil sludge degrading bacteria from production tank number 9 masjed soleiman |
| topic | Bioremediation Oil sludge Total petroleum hydrocarbons Gas chromatography |
| url | http://uijs.ui.ac.ir/bjm//browse.php?a_code=A-10-310-1&slc_lang=en&sid=1 |
| work_keys_str_mv | AT yaldasheyni isolationandidentificationofoilsludgedegradingbacteriafromproductiontanknumber9masjedsoleiman AT hosseinmotamedi isolationandidentificationofoilsludgedegradingbacteriafromproductiontanknumber9masjedsoleiman AT ahmadalipourbabaei isolationandidentificationofoilsludgedegradingbacteriafromproductiontanknumber9masjedsoleiman |