SARS-CoV-2 detection by targeting four loci of viral genome using graphene oxide and gold nanoparticle DNA biosensor
Abstract The current COVID-19 pandemic outbreak poses a serious threat to public health, demonstrating the critical need for the development of effective and reproducible detection tests. Since the RT-qPCR primers are highly specific and can only be designed based on the known sequence, mutation sen...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-11-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-23996-y |
| _version_ | 1811180251971059712 |
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| author | Arman Amani Babadi Shahrooz Rahmati Rafieh Fakhlaei Reza Heidari Saeid Baradaran Mostafa Akbariqomi Shuang Wang Gholamreza Tavoosidana William Doherty Kostya Ostrikov |
| author_facet | Arman Amani Babadi Shahrooz Rahmati Rafieh Fakhlaei Reza Heidari Saeid Baradaran Mostafa Akbariqomi Shuang Wang Gholamreza Tavoosidana William Doherty Kostya Ostrikov |
| author_sort | Arman Amani Babadi |
| collection | DOAJ |
| description | Abstract The current COVID-19 pandemic outbreak poses a serious threat to public health, demonstrating the critical need for the development of effective and reproducible detection tests. Since the RT-qPCR primers are highly specific and can only be designed based on the known sequence, mutation sensitivity is its limitation. Moreover, the mutations in the severe acute respiratory syndrome β-coronavirus (SARS-CoV-2) genome led to new highly transmissible variants such as Delta and Omicron variants. In the case of mutation, RT-qPCR primers cannot recognize and attach to the target sequence. This research presents an accurate dual-platform DNA biosensor based on the colorimetric assay of gold nanoparticles and the surface-enhanced Raman scattering (SERS) technique. It simultaneously targets four different regions of the viral genome for detection of SARS-CoV-2 and its new variants prior to any sequencing. Hence, in the case of mutation in one of the target sequences, the other three probes could detect the SARS-CoV-2 genome. The method is based on visible biosensor color shift and a locally enhanced electromagnetic field and significantly amplified SERS signal due to the proximity of Sulfo-Cyanine 3 (Cy3) and AuNPs intensity peak at 1468 cm-1. The dual-platform DNA/GO/AuNP biosensor exhibits high sensitivity toward the viral genome with a LOD of 0.16 ng/µL. This is a safe point-of-care, naked-eye, equipment-free, and rapid (10 min) detection biosensor for diagnosing COVID-19 cases at home using a nasopharyngeal sample. |
| first_indexed | 2024-04-11T06:48:12Z |
| format | Article |
| id | doaj.art-a803d3092c794629a35ac12bc07056a8 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-11T06:48:12Z |
| publishDate | 2022-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-a803d3092c794629a35ac12bc07056a82022-12-22T04:39:18ZengNature PortfolioScientific Reports2045-23222022-11-0112111210.1038/s41598-022-23996-ySARS-CoV-2 detection by targeting four loci of viral genome using graphene oxide and gold nanoparticle DNA biosensorArman Amani Babadi0Shahrooz Rahmati1Rafieh Fakhlaei2Reza Heidari3Saeid Baradaran4Mostafa Akbariqomi5Shuang Wang6Gholamreza Tavoosidana7William Doherty8Kostya Ostrikov9School of Energy and Power Engineering, Jiangsu UniversitySchool of Chemistry and Physics, Queensland University of Technology (QUT)Food Safety and Food Integrity (FOSFI), Institute of Tropical Agriculture and Food Security, Universiti Putra MalaysiaResearch Center for Cancer Screening and Epidemiology, AJA University of Medical SciencesNew Technologies Research Center, Amirkabir University of TechnologyApplied Microbiology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical SciencesSchool of Energy and Power Engineering, Jiangsu UniversityDepartment of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical SciencesCentre for Agriculture and the Bioeconomy, Queensland University of Technology (QUT)School of Chemistry and Physics, Queensland University of Technology (QUT)Abstract The current COVID-19 pandemic outbreak poses a serious threat to public health, demonstrating the critical need for the development of effective and reproducible detection tests. Since the RT-qPCR primers are highly specific and can only be designed based on the known sequence, mutation sensitivity is its limitation. Moreover, the mutations in the severe acute respiratory syndrome β-coronavirus (SARS-CoV-2) genome led to new highly transmissible variants such as Delta and Omicron variants. In the case of mutation, RT-qPCR primers cannot recognize and attach to the target sequence. This research presents an accurate dual-platform DNA biosensor based on the colorimetric assay of gold nanoparticles and the surface-enhanced Raman scattering (SERS) technique. It simultaneously targets four different regions of the viral genome for detection of SARS-CoV-2 and its new variants prior to any sequencing. Hence, in the case of mutation in one of the target sequences, the other three probes could detect the SARS-CoV-2 genome. The method is based on visible biosensor color shift and a locally enhanced electromagnetic field and significantly amplified SERS signal due to the proximity of Sulfo-Cyanine 3 (Cy3) and AuNPs intensity peak at 1468 cm-1. The dual-platform DNA/GO/AuNP biosensor exhibits high sensitivity toward the viral genome with a LOD of 0.16 ng/µL. This is a safe point-of-care, naked-eye, equipment-free, and rapid (10 min) detection biosensor for diagnosing COVID-19 cases at home using a nasopharyngeal sample.https://doi.org/10.1038/s41598-022-23996-y |
| spellingShingle | Arman Amani Babadi Shahrooz Rahmati Rafieh Fakhlaei Reza Heidari Saeid Baradaran Mostafa Akbariqomi Shuang Wang Gholamreza Tavoosidana William Doherty Kostya Ostrikov SARS-CoV-2 detection by targeting four loci of viral genome using graphene oxide and gold nanoparticle DNA biosensor Scientific Reports |
| title | SARS-CoV-2 detection by targeting four loci of viral genome using graphene oxide and gold nanoparticle DNA biosensor |
| title_full | SARS-CoV-2 detection by targeting four loci of viral genome using graphene oxide and gold nanoparticle DNA biosensor |
| title_fullStr | SARS-CoV-2 detection by targeting four loci of viral genome using graphene oxide and gold nanoparticle DNA biosensor |
| title_full_unstemmed | SARS-CoV-2 detection by targeting four loci of viral genome using graphene oxide and gold nanoparticle DNA biosensor |
| title_short | SARS-CoV-2 detection by targeting four loci of viral genome using graphene oxide and gold nanoparticle DNA biosensor |
| title_sort | sars cov 2 detection by targeting four loci of viral genome using graphene oxide and gold nanoparticle dna biosensor |
| url | https://doi.org/10.1038/s41598-022-23996-y |
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