mRNA vaccines induce rapid antibody responses in mice
Abstract mRNA vaccines can be developed and produced quickly, making them prime candidates for immediate outbreak responses. Furthermore, clinical trials have demonstrated rapid protection following mRNA vaccination. Thus, we sought to investigate how quickly mRNA vaccines elicit antibody responses...
| Հիմնական հեղինակներ: | , , , , , , , , , |
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| Ձևաչափ: | Հոդված |
| Լեզու: | English |
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Nature Portfolio
2022-08-01
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| Շարք: | npj Vaccines |
| Առցանց հասանելիություն: | https://doi.org/10.1038/s41541-022-00511-y |
| _version_ | 1827776029107683328 |
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| author | Makda S. Gebre Susanne Rauch Nicole Roth Janina Gergen Jingyou Yu Xiaowen Liu Andrew C. Cole Stefan O. Mueller Benjamin Petsch Dan H. Barouch |
| author_facet | Makda S. Gebre Susanne Rauch Nicole Roth Janina Gergen Jingyou Yu Xiaowen Liu Andrew C. Cole Stefan O. Mueller Benjamin Petsch Dan H. Barouch |
| author_sort | Makda S. Gebre |
| collection | DOAJ |
| description | Abstract mRNA vaccines can be developed and produced quickly, making them prime candidates for immediate outbreak responses. Furthermore, clinical trials have demonstrated rapid protection following mRNA vaccination. Thus, we sought to investigate how quickly mRNA vaccines elicit antibody responses compared to other vaccine modalities. We first compared the immune kinetics of mRNA and DNA vaccines expressing SARS-CoV-2 spike in mice. We observed rapid induction of antigen-specific binding and neutralizing antibodies by day 5 following mRNA (4 µg/mouse), but not DNA (50 µg/mouse), immunization. Comparing innate responses hours post immunization, the mRNA vaccine induced increased levels of IL-5, IL-6, and MCP-1 cytokines which maybe promoting humoral responses downstream. We then evaluated the immune kinetics of an HIV-1 mRNA vaccine in comparison to DNA, protein, and rhesus adenovirus 52 (RhAd52) vaccines of the same HIV-1 envelope antigen in mice. Again, induction of envelope-specific antibodies was observed by day 5 following mRNA vaccination, whereas antibodies were detected by day 7–14 following DNA, protein, and RhAd52 vaccination. Thus, eliciting rapid humoral immunity may be a unique and advantageous property of mRNA vaccines for controlling infectious disease outbreaks. |
| first_indexed | 2024-03-11T13:58:21Z |
| format | Article |
| id | doaj.art-a805989bd87b42629b9dba91cb15c05e |
| institution | Directory Open Access Journal |
| issn | 2059-0105 |
| language | English |
| last_indexed | 2024-03-11T13:58:21Z |
| publishDate | 2022-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | npj Vaccines |
| spelling | doaj.art-a805989bd87b42629b9dba91cb15c05e2023-11-02T05:43:36ZengNature Portfolionpj Vaccines2059-01052022-08-01711710.1038/s41541-022-00511-ymRNA vaccines induce rapid antibody responses in miceMakda S. Gebre0Susanne Rauch1Nicole Roth2Janina Gergen3Jingyou Yu4Xiaowen Liu5Andrew C. Cole6Stefan O. Mueller7Benjamin Petsch8Dan H. Barouch9Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical SchoolCureVac AGCureVac AGCureVac AGCenter for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical SchoolDepartment of Emergency Medicine, Beth Israel Deaconess Medical CenterDepartment of Emergency Medicine, Beth Israel Deaconess Medical CenterCureVac AGCureVac AGCenter for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical SchoolAbstract mRNA vaccines can be developed and produced quickly, making them prime candidates for immediate outbreak responses. Furthermore, clinical trials have demonstrated rapid protection following mRNA vaccination. Thus, we sought to investigate how quickly mRNA vaccines elicit antibody responses compared to other vaccine modalities. We first compared the immune kinetics of mRNA and DNA vaccines expressing SARS-CoV-2 spike in mice. We observed rapid induction of antigen-specific binding and neutralizing antibodies by day 5 following mRNA (4 µg/mouse), but not DNA (50 µg/mouse), immunization. Comparing innate responses hours post immunization, the mRNA vaccine induced increased levels of IL-5, IL-6, and MCP-1 cytokines which maybe promoting humoral responses downstream. We then evaluated the immune kinetics of an HIV-1 mRNA vaccine in comparison to DNA, protein, and rhesus adenovirus 52 (RhAd52) vaccines of the same HIV-1 envelope antigen in mice. Again, induction of envelope-specific antibodies was observed by day 5 following mRNA vaccination, whereas antibodies were detected by day 7–14 following DNA, protein, and RhAd52 vaccination. Thus, eliciting rapid humoral immunity may be a unique and advantageous property of mRNA vaccines for controlling infectious disease outbreaks.https://doi.org/10.1038/s41541-022-00511-y |
| spellingShingle | Makda S. Gebre Susanne Rauch Nicole Roth Janina Gergen Jingyou Yu Xiaowen Liu Andrew C. Cole Stefan O. Mueller Benjamin Petsch Dan H. Barouch mRNA vaccines induce rapid antibody responses in mice npj Vaccines |
| title | mRNA vaccines induce rapid antibody responses in mice |
| title_full | mRNA vaccines induce rapid antibody responses in mice |
| title_fullStr | mRNA vaccines induce rapid antibody responses in mice |
| title_full_unstemmed | mRNA vaccines induce rapid antibody responses in mice |
| title_short | mRNA vaccines induce rapid antibody responses in mice |
| title_sort | mrna vaccines induce rapid antibody responses in mice |
| url | https://doi.org/10.1038/s41541-022-00511-y |
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