Determination of Antimicrobial Properties of Crude Aqueous Leaves Extracts of Selected Medicinal Plants Using Resazurin-Based Microtiter Broth Dilution Method

The Philippines has a wide range of medicinal plants species that need to be tested for safety and efficacy for the possibility of serving as basis in drug development in the future. Five medicinal plants commonly used in the province of Cavite, Philippines were selected for crude aqueous leaf extra...

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Main Authors: Balinadoa L.O, Chanb M.A
Format: Article
Language:English
Published: School of Medicine and Pharmacy, Rabat, Morocco 2018-10-01
Series:Arabian Journal of Medicinal and Aromatic Plants
Subjects:
Online Access:https://revues.imist.ma/index.php/AJMAP/article/view/13922
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author Balinadoa L.O
Chanb M.A
author_facet Balinadoa L.O
Chanb M.A
author_sort Balinadoa L.O
collection DOAJ
description The Philippines has a wide range of medicinal plants species that need to be tested for safety and efficacy for the possibility of serving as basis in drug development in the future. Five medicinal plants commonly used in the province of Cavite, Philippines were selected for crude aqueous leaf extraction. These were Annona muricata L., Cymbopogon citratus DC., Graptophyllum pictum (L.) Griff, Jatropha curcas L. and Piper betle L. Extracts were tested for antimicrobial activities using resazurin-based microtiter broth dilution method. P. betle showed the maximum antimicrobial activity with minimum inhibitory concentrations (MIC) of 4.69 mg/ml against Pseudomonas aeruginosa and Staphylococcus aureus and 37.50 mg/ml against Candida albicans. This then validated the efficiency of P. betle in treating skin diseases and other infections as reported in the locality.
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spelling doaj.art-a807717abca74c27a6308c628a9e800d2022-12-21T23:58:03ZengSchool of Medicine and Pharmacy, Rabat, MoroccoArabian Journal of Medicinal and Aromatic Plants2458-59202018-10-014211410.48347/IMIST.PRSM/ajmap-v4i2.139227247Determination of Antimicrobial Properties of Crude Aqueous Leaves Extracts of Selected Medicinal Plants Using Resazurin-Based Microtiter Broth Dilution MethodBalinadoa L.O0Chanb M.A1Cavite State University, Indang, CaviteUniversity, Loyola Heights, Quezon CityThe Philippines has a wide range of medicinal plants species that need to be tested for safety and efficacy for the possibility of serving as basis in drug development in the future. Five medicinal plants commonly used in the province of Cavite, Philippines were selected for crude aqueous leaf extraction. These were Annona muricata L., Cymbopogon citratus DC., Graptophyllum pictum (L.) Griff, Jatropha curcas L. and Piper betle L. Extracts were tested for antimicrobial activities using resazurin-based microtiter broth dilution method. P. betle showed the maximum antimicrobial activity with minimum inhibitory concentrations (MIC) of 4.69 mg/ml against Pseudomonas aeruginosa and Staphylococcus aureus and 37.50 mg/ml against Candida albicans. This then validated the efficiency of P. betle in treating skin diseases and other infections as reported in the locality.https://revues.imist.ma/index.php/AJMAP/article/view/13922antimicrobial, microtiter broth dilution, minimum inhibitory concentration, resazurin
spellingShingle Balinadoa L.O
Chanb M.A
Determination of Antimicrobial Properties of Crude Aqueous Leaves Extracts of Selected Medicinal Plants Using Resazurin-Based Microtiter Broth Dilution Method
Arabian Journal of Medicinal and Aromatic Plants
antimicrobial, microtiter broth dilution, minimum inhibitory concentration, resazurin
title Determination of Antimicrobial Properties of Crude Aqueous Leaves Extracts of Selected Medicinal Plants Using Resazurin-Based Microtiter Broth Dilution Method
title_full Determination of Antimicrobial Properties of Crude Aqueous Leaves Extracts of Selected Medicinal Plants Using Resazurin-Based Microtiter Broth Dilution Method
title_fullStr Determination of Antimicrobial Properties of Crude Aqueous Leaves Extracts of Selected Medicinal Plants Using Resazurin-Based Microtiter Broth Dilution Method
title_full_unstemmed Determination of Antimicrobial Properties of Crude Aqueous Leaves Extracts of Selected Medicinal Plants Using Resazurin-Based Microtiter Broth Dilution Method
title_short Determination of Antimicrobial Properties of Crude Aqueous Leaves Extracts of Selected Medicinal Plants Using Resazurin-Based Microtiter Broth Dilution Method
title_sort determination of antimicrobial properties of crude aqueous leaves extracts of selected medicinal plants using resazurin based microtiter broth dilution method
topic antimicrobial, microtiter broth dilution, minimum inhibitory concentration, resazurin
url https://revues.imist.ma/index.php/AJMAP/article/view/13922
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