Reproductive Injury in Male Rats from Acrylamide Toxicity and Potential Protection by Earthworm Methanolic Extract
This study examined the protective effect of earthworm extract (EE) on acrylamide (ACR)-induced reproductive dysfunction. Forty male rats were allocated into four groups <i>(n</i> = 10). The G I (control) group received distilled water (D.W.). The G II group received ACR (5 mg kg<sup&...
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MDPI AG
2022-07-01
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| Online Access: | https://www.mdpi.com/2076-2615/12/13/1723 |
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| author | Mohamed M. Ahmed Amany A. Hammad Sahar H. Orabi Hamed T. Elbaz Ahmed E. Elweza Enas A. Tahoun Mona M. Elseehy Ahmed M. El-Shehawi Ahmed A. Mousa |
| author_facet | Mohamed M. Ahmed Amany A. Hammad Sahar H. Orabi Hamed T. Elbaz Ahmed E. Elweza Enas A. Tahoun Mona M. Elseehy Ahmed M. El-Shehawi Ahmed A. Mousa |
| author_sort | Mohamed M. Ahmed |
| collection | DOAJ |
| description | This study examined the protective effect of earthworm extract (EE) on acrylamide (ACR)-induced reproductive dysfunction. Forty male rats were allocated into four groups <i>(n</i> = 10). The G I (control) group received distilled water (D.W.). The G II group received ACR (5 mg kg<sup>−1</sup> B.W. in D.W.) 5 days per week, orally, for 3 weeks. The G III group was administered EE (300 mg kg<sup>−1</sup> B.W in D.W.) 5 days per week, orally, for 3 weeks. The G IV group was pretreated with EE for 3 weeks and then co-treated with EE and ACR for an additional 3 weeks. ACR decreased the number of sperm, sperm viability, and total motility. However, it increased testosterone levels with no effect on the FSH or LH levels. Moreover, ACR increased the concentrations of malondialdehyde (MDA) and nitric oxide (NO). Meanwhile, it decreased the glutathione (GSH) concentration in testicular tissues. Notably, the expression levels of p53 and Ki-67 were increased in the degenerated spermatogenic cells and in the hyperplastic Leydig cells of the testis of the ACR-treated group, respectively. Acrylamide induced alterations in the testicular tissue architecture. Interestingly, EE restored the sperm parameters and recovered the testicular histological structures and the biochemical alterations induced by ACR. In conclusion, earthworm extract ameliorated ACR-induced reproductive toxicity via restoring the testicular antioxidant balance and suppressing p53 and Ki-67 expressions in testicular tissues. |
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| issn | 2076-2615 |
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| last_indexed | 2024-03-09T22:10:37Z |
| publishDate | 2022-07-01 |
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| record_format | Article |
| series | Animals |
| spelling | doaj.art-a807f002079d42c7b2b178a974657fcb2023-11-23T19:33:53ZengMDPI AGAnimals2076-26152022-07-011213172310.3390/ani12131723Reproductive Injury in Male Rats from Acrylamide Toxicity and Potential Protection by Earthworm Methanolic ExtractMohamed M. Ahmed0Amany A. Hammad1Sahar H. Orabi2Hamed T. Elbaz3Ahmed E. Elweza4Enas A. Tahoun5Mona M. Elseehy6Ahmed M. El-Shehawi7Ahmed A. Mousa8Department of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary Medicine, University of Sadat City, Sadat City 32897, Menoufia, EgyptDepartment of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary Medicine, University of Sadat City, Sadat City 32897, Menoufia, EgyptDepartment of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary Medicine, University of Sadat City, Sadat City 32897, Menoufia, EgyptDepartment of Theriogenology, Faculty of Veterinary Medicine, University of Sadat City, Sadat City 32897, Menoufia, EgyptDepartment of Theriogenology, Faculty of Veterinary Medicine, University of Sadat City, Sadat City 32897, Menoufia, EgyptDepartment of Pathology, Faculty of Veterinary Medicine, University of Sadat City, Sadat City 32897, Menoufia, EgyptDepartment of Genetics, Faculty of Agriculture, University of Alexandria, Alexandria 21545, Alexandria, EgyptDepartment of Biotechnology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaDepartment of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary Medicine, University of Sadat City, Sadat City 32897, Menoufia, EgyptThis study examined the protective effect of earthworm extract (EE) on acrylamide (ACR)-induced reproductive dysfunction. Forty male rats were allocated into four groups <i>(n</i> = 10). The G I (control) group received distilled water (D.W.). The G II group received ACR (5 mg kg<sup>−1</sup> B.W. in D.W.) 5 days per week, orally, for 3 weeks. The G III group was administered EE (300 mg kg<sup>−1</sup> B.W in D.W.) 5 days per week, orally, for 3 weeks. The G IV group was pretreated with EE for 3 weeks and then co-treated with EE and ACR for an additional 3 weeks. ACR decreased the number of sperm, sperm viability, and total motility. However, it increased testosterone levels with no effect on the FSH or LH levels. Moreover, ACR increased the concentrations of malondialdehyde (MDA) and nitric oxide (NO). Meanwhile, it decreased the glutathione (GSH) concentration in testicular tissues. Notably, the expression levels of p53 and Ki-67 were increased in the degenerated spermatogenic cells and in the hyperplastic Leydig cells of the testis of the ACR-treated group, respectively. Acrylamide induced alterations in the testicular tissue architecture. Interestingly, EE restored the sperm parameters and recovered the testicular histological structures and the biochemical alterations induced by ACR. In conclusion, earthworm extract ameliorated ACR-induced reproductive toxicity via restoring the testicular antioxidant balance and suppressing p53 and Ki-67 expressions in testicular tissues.https://www.mdpi.com/2076-2615/12/13/1723acrylamideearthwormratsreproductive toxicity |
| spellingShingle | Mohamed M. Ahmed Amany A. Hammad Sahar H. Orabi Hamed T. Elbaz Ahmed E. Elweza Enas A. Tahoun Mona M. Elseehy Ahmed M. El-Shehawi Ahmed A. Mousa Reproductive Injury in Male Rats from Acrylamide Toxicity and Potential Protection by Earthworm Methanolic Extract Animals acrylamide earthworm rats reproductive toxicity |
| title | Reproductive Injury in Male Rats from Acrylamide Toxicity and Potential Protection by Earthworm Methanolic Extract |
| title_full | Reproductive Injury in Male Rats from Acrylamide Toxicity and Potential Protection by Earthworm Methanolic Extract |
| title_fullStr | Reproductive Injury in Male Rats from Acrylamide Toxicity and Potential Protection by Earthworm Methanolic Extract |
| title_full_unstemmed | Reproductive Injury in Male Rats from Acrylamide Toxicity and Potential Protection by Earthworm Methanolic Extract |
| title_short | Reproductive Injury in Male Rats from Acrylamide Toxicity and Potential Protection by Earthworm Methanolic Extract |
| title_sort | reproductive injury in male rats from acrylamide toxicity and potential protection by earthworm methanolic extract |
| topic | acrylamide earthworm rats reproductive toxicity |
| url | https://www.mdpi.com/2076-2615/12/13/1723 |
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