PagMYB205 Negatively Affects Poplar Salt Tolerance through Reactive Oxygen Species Scavenging and Root Vitality Modulation
Salt stress is one of the major abiotic stresses that limits plant growth and development. The MYB transcription factor family plays essential roles in plant growth and development, as well as stress tolerance processes. In this study, the cDNA of the 84K poplar (<i>Populus abla</i> × &l...
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MDPI AG
2023-10-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/20/15437 |
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| author | Lieding Zhou Xuhui Huan Kai Zhao Xia Jin Jia Hu Shuhui Du Youzhi Han Shengji Wang |
| author_facet | Lieding Zhou Xuhui Huan Kai Zhao Xia Jin Jia Hu Shuhui Du Youzhi Han Shengji Wang |
| author_sort | Lieding Zhou |
| collection | DOAJ |
| description | Salt stress is one of the major abiotic stresses that limits plant growth and development. The MYB transcription factor family plays essential roles in plant growth and development, as well as stress tolerance processes. In this study, the cDNA of the 84K poplar (<i>Populus abla</i> × <i>Populus glandulosa</i>) was used as a template to clone the full length of the <i>PagMYB205</i> gene fragment, and transgenic poplar lines with <i>PagMYB205</i> overexpression (OX) or inhibited expression (RNAi, RNA interference) were cultivated. The role of PagMYB205 in poplar growth and development and salt tolerance was detected using morphological and physiological methods. The full-length CDS sequence of <i>PagMYB205</i> was 906 bp, encoding 301 amino acids, and the upstream promoter sequence contained abiotic stress-related cis-acting elements. The results of subcellular localization and transactivation assays showed that the protein had no self-activating activity and was localized in the nucleus. Under salt stress, the rooting rate and root vitality of RNAi were higher than OX and wild type (WT). However, the malondialdehyde (MDA) content of the RNAi lines was significantly lower than that of the wild-type (WT) and OX lines, but the reactive oxygen species (ROS) scavenging ability, such as the peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT) enzyme activities, was dramatically more powerful. Most significantly of all, the RNAi3 line with the lowest expression level of <i>PagMYB205</i> had the lowest MDA content, the best enzyme activity and root vitality, and the best salt stress tolerance compared to the other lines. The above results suggest that the transcription factor PagMYB205 could negatively regulate salt stress tolerance by regulating antioxidant enzyme activity and root vitality. |
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| spelling | doaj.art-a80a847e48154575a47cd0b7fd81e23a2023-11-19T16:46:44ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-10-0124201543710.3390/ijms242015437PagMYB205 Negatively Affects Poplar Salt Tolerance through Reactive Oxygen Species Scavenging and Root Vitality ModulationLieding Zhou0Xuhui Huan1Kai Zhao2Xia Jin3Jia Hu4Shuhui Du5Youzhi Han6Shengji Wang7College of Forestry, Shanxi Agricultural University, Jinzhong 030801, ChinaCollege of Forestry, Shanxi Agricultural University, Jinzhong 030801, ChinaCollege of Forestry, Shanxi Agricultural University, Jinzhong 030801, ChinaCollege of Forestry, Shanxi Agricultural University, Jinzhong 030801, ChinaCollege of Forestry, Shanxi Agricultural University, Jinzhong 030801, ChinaCollege of Forestry, Shanxi Agricultural University, Jinzhong 030801, ChinaCollege of Forestry, Shanxi Agricultural University, Jinzhong 030801, ChinaCollege of Forestry, Shanxi Agricultural University, Jinzhong 030801, ChinaSalt stress is one of the major abiotic stresses that limits plant growth and development. The MYB transcription factor family plays essential roles in plant growth and development, as well as stress tolerance processes. In this study, the cDNA of the 84K poplar (<i>Populus abla</i> × <i>Populus glandulosa</i>) was used as a template to clone the full length of the <i>PagMYB205</i> gene fragment, and transgenic poplar lines with <i>PagMYB205</i> overexpression (OX) or inhibited expression (RNAi, RNA interference) were cultivated. The role of PagMYB205 in poplar growth and development and salt tolerance was detected using morphological and physiological methods. The full-length CDS sequence of <i>PagMYB205</i> was 906 bp, encoding 301 amino acids, and the upstream promoter sequence contained abiotic stress-related cis-acting elements. The results of subcellular localization and transactivation assays showed that the protein had no self-activating activity and was localized in the nucleus. Under salt stress, the rooting rate and root vitality of RNAi were higher than OX and wild type (WT). However, the malondialdehyde (MDA) content of the RNAi lines was significantly lower than that of the wild-type (WT) and OX lines, but the reactive oxygen species (ROS) scavenging ability, such as the peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT) enzyme activities, was dramatically more powerful. Most significantly of all, the RNAi3 line with the lowest expression level of <i>PagMYB205</i> had the lowest MDA content, the best enzyme activity and root vitality, and the best salt stress tolerance compared to the other lines. The above results suggest that the transcription factor PagMYB205 could negatively regulate salt stress tolerance by regulating antioxidant enzyme activity and root vitality.https://www.mdpi.com/1422-0067/24/20/15437<i>Populus abla × Populus glandulosa</i>MYB transcription factorsalt stressantioxidant enzymesroot vitality |
| spellingShingle | Lieding Zhou Xuhui Huan Kai Zhao Xia Jin Jia Hu Shuhui Du Youzhi Han Shengji Wang PagMYB205 Negatively Affects Poplar Salt Tolerance through Reactive Oxygen Species Scavenging and Root Vitality Modulation International Journal of Molecular Sciences <i>Populus abla × Populus glandulosa</i> MYB transcription factor salt stress antioxidant enzymes root vitality |
| title | PagMYB205 Negatively Affects Poplar Salt Tolerance through Reactive Oxygen Species Scavenging and Root Vitality Modulation |
| title_full | PagMYB205 Negatively Affects Poplar Salt Tolerance through Reactive Oxygen Species Scavenging and Root Vitality Modulation |
| title_fullStr | PagMYB205 Negatively Affects Poplar Salt Tolerance through Reactive Oxygen Species Scavenging and Root Vitality Modulation |
| title_full_unstemmed | PagMYB205 Negatively Affects Poplar Salt Tolerance through Reactive Oxygen Species Scavenging and Root Vitality Modulation |
| title_short | PagMYB205 Negatively Affects Poplar Salt Tolerance through Reactive Oxygen Species Scavenging and Root Vitality Modulation |
| title_sort | pagmyb205 negatively affects poplar salt tolerance through reactive oxygen species scavenging and root vitality modulation |
| topic | <i>Populus abla × Populus glandulosa</i> MYB transcription factor salt stress antioxidant enzymes root vitality |
| url | https://www.mdpi.com/1422-0067/24/20/15437 |
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