Evolutionary consequences of intra-patient phage predation on microbial populations
The impact of phage predation on bacterial pathogens in the context of human disease is not currently appreciated. Here, we show that predatory interactions of a phage with an important environmentally transmitted pathogen, Vibrio cholerae, can modulate the evolutionary trajectory of this pathogen d...
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2014-08-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/03497 |
| _version_ | 1811235862170566656 |
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| author | Kimberley D Seed Minmin Yen B Jesse Shapiro Isabelle J Hilaire Richelle C Charles Jessica E Teng Louise C Ivers Jacques Boncy Jason B Harris Andrew Camilli |
| author_facet | Kimberley D Seed Minmin Yen B Jesse Shapiro Isabelle J Hilaire Richelle C Charles Jessica E Teng Louise C Ivers Jacques Boncy Jason B Harris Andrew Camilli |
| author_sort | Kimberley D Seed |
| collection | DOAJ |
| description | The impact of phage predation on bacterial pathogens in the context of human disease is not currently appreciated. Here, we show that predatory interactions of a phage with an important environmentally transmitted pathogen, Vibrio cholerae, can modulate the evolutionary trajectory of this pathogen during the natural course of infection within individual patients. We analyzed geographically and temporally disparate cholera patient stool samples from Haiti and Bangladesh and found that phage predation can drive the genomic diversity of intra-patient V. cholerae populations. Intra-patient phage-sensitive and phage-resistant isolates were isogenic except for mutations conferring phage resistance, and moreover, phage-resistant V. cholerae populations were composed of a heterogeneous mix of many unique mutants. We also observed that phage predation can significantly alter the virulence potential of V. cholerae shed from cholera patients. We provide the first molecular evidence for predatory phage shaping microbial community structure during the natural course of infection in humans. |
| first_indexed | 2024-04-12T11:59:22Z |
| format | Article |
| id | doaj.art-a80bbcce4b904e378dbe1e2c2d25f265 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T11:59:22Z |
| publishDate | 2014-08-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-a80bbcce4b904e378dbe1e2c2d25f2652022-12-22T03:33:53ZengeLife Sciences Publications LtdeLife2050-084X2014-08-01310.7554/eLife.03497Evolutionary consequences of intra-patient phage predation on microbial populationsKimberley D Seed0Minmin Yen1B Jesse Shapiro2Isabelle J Hilaire3Richelle C Charles4Jessica E Teng5Louise C Ivers6Jacques Boncy7Jason B Harris8Andrew Camilli9Department of Molecular Biology and Microbiology, Howard Hughes Medical Institute, Tufts University School of Medicine, Boston, United StatesDepartment of Molecular Biology and Microbiology, Howard Hughes Medical Institute, Tufts University School of Medicine, Boston, United StatesDépartement de sciences biologiques, Université de Montréal, Montreal, CanadaPartners In Health, Boston, United StatesDivision of Infectious Diseases, Massachusetts General Hospital, Boston, United States; Department of Medicine, Harvard Medical School, Boston, United StatesPartners In Health, Boston, United States; Division of Global Health Equity, Brigham and Women's Hospital, Boston, United StatesPartners In Health, Boston, United States; Division of Global Health Equity, Brigham and Women's Hospital, Boston, United States; Department of Global Health and Social Medicine, Harvard Medical School, Boston, United StatesNational Public Health Laboratory, Port-au-Prince, HaitiDivision of Infectious Diseases, Massachusetts General Hospital, Boston, United States; Department of Medicine, Harvard Medical School, Boston, United States; Department of Pediatrics, Harvard Medical School, Boston, United StatesDepartment of Molecular Biology and Microbiology, Howard Hughes Medical Institute, Tufts University School of Medicine, Boston, United StatesThe impact of phage predation on bacterial pathogens in the context of human disease is not currently appreciated. Here, we show that predatory interactions of a phage with an important environmentally transmitted pathogen, Vibrio cholerae, can modulate the evolutionary trajectory of this pathogen during the natural course of infection within individual patients. We analyzed geographically and temporally disparate cholera patient stool samples from Haiti and Bangladesh and found that phage predation can drive the genomic diversity of intra-patient V. cholerae populations. Intra-patient phage-sensitive and phage-resistant isolates were isogenic except for mutations conferring phage resistance, and moreover, phage-resistant V. cholerae populations were composed of a heterogeneous mix of many unique mutants. We also observed that phage predation can significantly alter the virulence potential of V. cholerae shed from cholera patients. We provide the first molecular evidence for predatory phage shaping microbial community structure during the natural course of infection in humans.https://elifesciences.org/articles/03497Vibrio choleraecholerabacteriophagephageOmpUToxR |
| spellingShingle | Kimberley D Seed Minmin Yen B Jesse Shapiro Isabelle J Hilaire Richelle C Charles Jessica E Teng Louise C Ivers Jacques Boncy Jason B Harris Andrew Camilli Evolutionary consequences of intra-patient phage predation on microbial populations eLife Vibrio cholerae cholera bacteriophage phage OmpU ToxR |
| title | Evolutionary consequences of intra-patient phage predation on microbial populations |
| title_full | Evolutionary consequences of intra-patient phage predation on microbial populations |
| title_fullStr | Evolutionary consequences of intra-patient phage predation on microbial populations |
| title_full_unstemmed | Evolutionary consequences of intra-patient phage predation on microbial populations |
| title_short | Evolutionary consequences of intra-patient phage predation on microbial populations |
| title_sort | evolutionary consequences of intra patient phage predation on microbial populations |
| topic | Vibrio cholerae cholera bacteriophage phage OmpU ToxR |
| url | https://elifesciences.org/articles/03497 |
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