Experimental investigation of freeze injury temperatures in trees and their contributing factors based on electrical impedance spectroscopy
In trees, injuries resulting from subfreezing temperatures can cause damage to the cellular biofilm system, metabolic functions, and fibrous reticulum, and even cell death. Investigating the occurrence of freezing damage and its contributing factors could help understand the mechanisms underlying fr...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-02-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2024.1326038/full |
| _version_ | 1797316001194311680 |
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| author | Xinyu Song Tong Gao Mengyao Ai Shan Gao |
| author_facet | Xinyu Song Tong Gao Mengyao Ai Shan Gao |
| author_sort | Xinyu Song |
| collection | DOAJ |
| description | In trees, injuries resulting from subfreezing temperatures can cause damage to the cellular biofilm system, metabolic functions, and fibrous reticulum, and even cell death. Investigating the occurrence of freezing damage and its contributing factors could help understand the mechanisms underlying freezing injury and prevent the subsequent damage in trees. To achieve this, a laboratory experiment was conducted using cut wood samples from Korean pine (Pinus koraiensis Siebold & Zucc) and Simon poplar (Populus simonii Carr.), and the effects of environmental freezing factors, including freezing temperatures, freezing duration, and cooling rate, on the temperature at which freezing injuries occur were examined using the electrical impedance spectroscopy (EIS) method. The semi-lethal temperature (LT50), as an indicator of freezing injury in wood tissue, was theoretically deduced based on the measured extracellular resistance (re) using EIS. The contributory factors to changes in LT50 were determined and their relationship was established. The results revealed that all freezing factors exhibited significant effects on electrical impedance characteristics (re, ri, and τ), significantly influencing the LT50 of the wood. Random forest (RF) and support vector machine (SVM) models were used to assess the contribution of the freezing factors and moisture content (MC). Among the factors examined, freezing duration had the greatest impact on LT50, followed by the MC, whereas the contribution of the cooling rate was minimal. The model accuracies were 0.89 and 0.86 for Korean pine and Simon poplar, respectively. The findings of our study illustrate that the occurrence of freezing injury in trees is primarily influenced by the duration of freezing at specific subzero temperatures. Slow cooling combined with prolonged freezing at low subzero temperatures leads to earlier and more severe freezing damage. |
| first_indexed | 2024-03-08T03:12:00Z |
| format | Article |
| id | doaj.art-a80da2a43f7a4b81b468ae8d9093ed89 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-03-08T03:12:00Z |
| publishDate | 2024-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-a80da2a43f7a4b81b468ae8d9093ed892024-02-13T04:38:30ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2024-02-011510.3389/fpls.2024.13260381326038Experimental investigation of freeze injury temperatures in trees and their contributing factors based on electrical impedance spectroscopyXinyu Song0Tong Gao1Mengyao Ai2Shan Gao3College of Mechanical and Electrical Engineering, Northeast Forestry University, Harbin, ChinaCollege of Mechanical and Electrical Engineering, Northeast Forestry University, Harbin, ChinaCollege of Mechanical and Electrical Engineering, Northeast Forestry University, Harbin, ChinaSchool of Civil Engineering and Transportation, Northeast Forestry University, Harbin, ChinaIn trees, injuries resulting from subfreezing temperatures can cause damage to the cellular biofilm system, metabolic functions, and fibrous reticulum, and even cell death. Investigating the occurrence of freezing damage and its contributing factors could help understand the mechanisms underlying freezing injury and prevent the subsequent damage in trees. To achieve this, a laboratory experiment was conducted using cut wood samples from Korean pine (Pinus koraiensis Siebold & Zucc) and Simon poplar (Populus simonii Carr.), and the effects of environmental freezing factors, including freezing temperatures, freezing duration, and cooling rate, on the temperature at which freezing injuries occur were examined using the electrical impedance spectroscopy (EIS) method. The semi-lethal temperature (LT50), as an indicator of freezing injury in wood tissue, was theoretically deduced based on the measured extracellular resistance (re) using EIS. The contributory factors to changes in LT50 were determined and their relationship was established. The results revealed that all freezing factors exhibited significant effects on electrical impedance characteristics (re, ri, and τ), significantly influencing the LT50 of the wood. Random forest (RF) and support vector machine (SVM) models were used to assess the contribution of the freezing factors and moisture content (MC). Among the factors examined, freezing duration had the greatest impact on LT50, followed by the MC, whereas the contribution of the cooling rate was minimal. The model accuracies were 0.89 and 0.86 for Korean pine and Simon poplar, respectively. The findings of our study illustrate that the occurrence of freezing injury in trees is primarily influenced by the duration of freezing at specific subzero temperatures. Slow cooling combined with prolonged freezing at low subzero temperatures leads to earlier and more severe freezing damage.https://www.frontiersin.org/articles/10.3389/fpls.2024.1326038/fullfreeze injurysemi-lethal temperatureelectrical impedance spectroscopycooling ratefreezing duration |
| spellingShingle | Xinyu Song Tong Gao Mengyao Ai Shan Gao Experimental investigation of freeze injury temperatures in trees and their contributing factors based on electrical impedance spectroscopy Frontiers in Plant Science freeze injury semi-lethal temperature electrical impedance spectroscopy cooling rate freezing duration |
| title | Experimental investigation of freeze injury temperatures in trees and their contributing factors based on electrical impedance spectroscopy |
| title_full | Experimental investigation of freeze injury temperatures in trees and their contributing factors based on electrical impedance spectroscopy |
| title_fullStr | Experimental investigation of freeze injury temperatures in trees and their contributing factors based on electrical impedance spectroscopy |
| title_full_unstemmed | Experimental investigation of freeze injury temperatures in trees and their contributing factors based on electrical impedance spectroscopy |
| title_short | Experimental investigation of freeze injury temperatures in trees and their contributing factors based on electrical impedance spectroscopy |
| title_sort | experimental investigation of freeze injury temperatures in trees and their contributing factors based on electrical impedance spectroscopy |
| topic | freeze injury semi-lethal temperature electrical impedance spectroscopy cooling rate freezing duration |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2024.1326038/full |
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