Pro-Osteogenic Properties of <i>Violina pumpkin</i> (<i>Cucurbita moschata</i>) Leaf Extracts: Data from In Vitro Human Primary Cell Cultures
Traditional medicines rely mainly on use of plant extracts to mitigate or treat a wide range of disorders, including those that affect skeletal homeostasis. In this study, we investigated for the first time the potential pro-osteogenic effects of hexane, acetone and methanol extracts of the leaves o...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-07-01
|
| Series: | Nutrients |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2072-6643/13/8/2633 |
| _version_ | 1797522579360055296 |
|---|---|
| author | Elisabetta Lambertini Letizia Penolazzi Giulia Pellielo Caterina Pipino Assunta Pandolfi Serena Fiorito Francesco Epifano Salvatore Genovese Roberta Piva |
| author_facet | Elisabetta Lambertini Letizia Penolazzi Giulia Pellielo Caterina Pipino Assunta Pandolfi Serena Fiorito Francesco Epifano Salvatore Genovese Roberta Piva |
| author_sort | Elisabetta Lambertini |
| collection | DOAJ |
| description | Traditional medicines rely mainly on use of plant extracts to mitigate or treat a wide range of disorders, including those that affect skeletal homeostasis. In this study, we investigated for the first time the potential pro-osteogenic effects of hexane, acetone and methanol extracts of the leaves of Cucurbita moschata, a very popular pumpkin cultivar in Western countries. We found that in Cucurbita moschata leaves, there are acetone-extractable substances—in particular, fatty acids such as 13-OH-9Z,11E,15E-octadecatrienoic acid (PU-13OH-FA), which is capable of both stimulating the function of human primary osteoblasts, which are responsible for bone formation, and inhibiting the differentiation of human osteoclasts, which are responsible for bone resorption. This dual effect was monitored by analyzing Runx2 expression, deposition of mineralized matrix, ALP activity, TRAP and actin ring staining respectively. This study suggests that bioactive chemicals from Cucurbita moschata leaves are potentially suitable as therapeutics for managing metabolic bone disorders such as osteoporosis and rheumatoid arthritis, and promoting tissue healing and functional recovery after bone fractures. The data we obtained increase knowledge on the biological activities of Cucurbita moschata, and in particular underline the potential benefits of consuming leaves which are a part of the plant currently little considered in the Western world. |
| first_indexed | 2024-03-10T08:31:26Z |
| format | Article |
| id | doaj.art-a8123de3e5c2447b89c7b46d60f15c5e |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-10T08:31:26Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-a8123de3e5c2447b89c7b46d60f15c5e2023-11-22T09:03:40ZengMDPI AGNutrients2072-66432021-07-01138263310.3390/nu13082633Pro-Osteogenic Properties of <i>Violina pumpkin</i> (<i>Cucurbita moschata</i>) Leaf Extracts: Data from In Vitro Human Primary Cell CulturesElisabetta Lambertini0Letizia Penolazzi1Giulia Pellielo2Caterina Pipino3Assunta Pandolfi4Serena Fiorito5Francesco Epifano6Salvatore Genovese7Roberta Piva8Department of Neuroscience and Rehabilitation, University of Ferrara, 44121 Ferrara, ItalyDepartment of Neuroscience and Rehabilitation, University of Ferrara, 44121 Ferrara, ItalyDepartment of Neuroscience and Rehabilitation, University of Ferrara, 44121 Ferrara, ItalyDepartment of Medical, Oral and Biotechnological Sciences, Center for Advanced Studies and Technology—CAST, University G. d’Annunzio of Chieti-Pescara, 66100 Chieti, ItalyDepartment of Medical, Oral and Biotechnological Sciences, Center for Advanced Studies and Technology—CAST, University G. d’Annunzio of Chieti-Pescara, 66100 Chieti, ItalyDepartment of Pharmacy, University “G. D’Annunzio” of Chieti-Pescara, 66100 Chieti, ItalyDepartment of Pharmacy, University “G. D’Annunzio” of Chieti-Pescara, 66100 Chieti, ItalyDepartment of Pharmacy, University “G. D’Annunzio” of Chieti-Pescara, 66100 Chieti, ItalyDepartment of Neuroscience and Rehabilitation, University of Ferrara, 44121 Ferrara, ItalyTraditional medicines rely mainly on use of plant extracts to mitigate or treat a wide range of disorders, including those that affect skeletal homeostasis. In this study, we investigated for the first time the potential pro-osteogenic effects of hexane, acetone and methanol extracts of the leaves of Cucurbita moschata, a very popular pumpkin cultivar in Western countries. We found that in Cucurbita moschata leaves, there are acetone-extractable substances—in particular, fatty acids such as 13-OH-9Z,11E,15E-octadecatrienoic acid (PU-13OH-FA), which is capable of both stimulating the function of human primary osteoblasts, which are responsible for bone formation, and inhibiting the differentiation of human osteoclasts, which are responsible for bone resorption. This dual effect was monitored by analyzing Runx2 expression, deposition of mineralized matrix, ALP activity, TRAP and actin ring staining respectively. This study suggests that bioactive chemicals from Cucurbita moschata leaves are potentially suitable as therapeutics for managing metabolic bone disorders such as osteoporosis and rheumatoid arthritis, and promoting tissue healing and functional recovery after bone fractures. The data we obtained increase knowledge on the biological activities of Cucurbita moschata, and in particular underline the potential benefits of consuming leaves which are a part of the plant currently little considered in the Western world.https://www.mdpi.com/2072-6643/13/8/2633pumpkin leaf extractsosteoblastsosteoclasts |
| spellingShingle | Elisabetta Lambertini Letizia Penolazzi Giulia Pellielo Caterina Pipino Assunta Pandolfi Serena Fiorito Francesco Epifano Salvatore Genovese Roberta Piva Pro-Osteogenic Properties of <i>Violina pumpkin</i> (<i>Cucurbita moschata</i>) Leaf Extracts: Data from In Vitro Human Primary Cell Cultures Nutrients pumpkin leaf extracts osteoblasts osteoclasts |
| title | Pro-Osteogenic Properties of <i>Violina pumpkin</i> (<i>Cucurbita moschata</i>) Leaf Extracts: Data from In Vitro Human Primary Cell Cultures |
| title_full | Pro-Osteogenic Properties of <i>Violina pumpkin</i> (<i>Cucurbita moschata</i>) Leaf Extracts: Data from In Vitro Human Primary Cell Cultures |
| title_fullStr | Pro-Osteogenic Properties of <i>Violina pumpkin</i> (<i>Cucurbita moschata</i>) Leaf Extracts: Data from In Vitro Human Primary Cell Cultures |
| title_full_unstemmed | Pro-Osteogenic Properties of <i>Violina pumpkin</i> (<i>Cucurbita moschata</i>) Leaf Extracts: Data from In Vitro Human Primary Cell Cultures |
| title_short | Pro-Osteogenic Properties of <i>Violina pumpkin</i> (<i>Cucurbita moschata</i>) Leaf Extracts: Data from In Vitro Human Primary Cell Cultures |
| title_sort | pro osteogenic properties of i violina pumpkin i i cucurbita moschata i leaf extracts data from in vitro human primary cell cultures |
| topic | pumpkin leaf extracts osteoblasts osteoclasts |
| url | https://www.mdpi.com/2072-6643/13/8/2633 |
| work_keys_str_mv | AT elisabettalambertini proosteogenicpropertiesofiviolinapumpkiniicucurbitamoschataileafextractsdatafrominvitrohumanprimarycellcultures AT letiziapenolazzi proosteogenicpropertiesofiviolinapumpkiniicucurbitamoschataileafextractsdatafrominvitrohumanprimarycellcultures AT giuliapellielo proosteogenicpropertiesofiviolinapumpkiniicucurbitamoschataileafextractsdatafrominvitrohumanprimarycellcultures AT caterinapipino proosteogenicpropertiesofiviolinapumpkiniicucurbitamoschataileafextractsdatafrominvitrohumanprimarycellcultures AT assuntapandolfi proosteogenicpropertiesofiviolinapumpkiniicucurbitamoschataileafextractsdatafrominvitrohumanprimarycellcultures AT serenafiorito proosteogenicpropertiesofiviolinapumpkiniicucurbitamoschataileafextractsdatafrominvitrohumanprimarycellcultures AT francescoepifano proosteogenicpropertiesofiviolinapumpkiniicucurbitamoschataileafextractsdatafrominvitrohumanprimarycellcultures AT salvatoregenovese proosteogenicpropertiesofiviolinapumpkiniicucurbitamoschataileafextractsdatafrominvitrohumanprimarycellcultures AT robertapiva proosteogenicpropertiesofiviolinapumpkiniicucurbitamoschataileafextractsdatafrominvitrohumanprimarycellcultures |