KIF23 is a potential biomarker of diffuse large B cell lymphoma
Abstract. Diffuse Large B Cell Lymphoma (DLBCL), the most common form of blood cancer. The genetic and clinical heterogeneity of DLBCL poses a major barrier to diagnosis and treatment. Hence, we aim to identify potential biomarkers for DLBCL. Differentially expressed genes were screened between DLBC...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer
2022-06-01
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| Series: | Medicine |
| Online Access: | http://journals.lww.com/10.1097/MD.0000000000029312 |
| _version_ | 1818219179195498496 |
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| author | Yuqi Gong, PhD Lingna Zhou, MD Liya Ding, MD Jing Zhao, MD Zhe Wang, PhD Guoping Ren, PhD Jing Zhang, PhD Zhengrong Mao, PhD Ren Zhou, PhD |
| author_facet | Yuqi Gong, PhD Lingna Zhou, MD Liya Ding, MD Jing Zhao, MD Zhe Wang, PhD Guoping Ren, PhD Jing Zhang, PhD Zhengrong Mao, PhD Ren Zhou, PhD |
| author_sort | Yuqi Gong, PhD |
| collection | DOAJ |
| description | Abstract. Diffuse Large B Cell Lymphoma (DLBCL), the most common form of blood cancer. The genetic and clinical heterogeneity of DLBCL poses a major barrier to diagnosis and treatment. Hence, we aim to identify potential biomarkers for DLBCL.
Differentially expressed genes were screened between DLBCL and the corresponding normal tissues. Kyoto Encyclopedia of Genes and Genomes and Gene oncology analyses were performed to obtain an insight into these differentially expressed genes. PPI network was constructed to identify hub genes. survival analysis was applied to evaluate the prognostic value of those hub genes. DNA methylation analysis was implemented to explore the epigenetic dysregulation of genes in DLBCL.
In this study, Kinesin family member 23 (KIF23) showed higher expression in DLBCL and was identified as a risk factor in DLBCL. The immunohistochemistry experiment further confirmed this finding. Subsequently, the univariate and multivariate analysis indicated that KIF23 might be an independent adverse factor in DLBCL. Upregulation of KIF23 might be a risk factor for the overall survival of patients who received an R-CHOP regimen, in late-stage, whatever with or without extranodal sites. Higher expression of KIF23 also significantly reduced 3, 5, 10-year overall survival. Furthermore, functional enrichment analyses (Kyoto Encyclopedia of Genes and Genomes, Gene oncology, and Gene Set Enrichment Analysis) showed that KIF23 was mainly involved in cell cycle, nuclear division, PI3K/AKT/mTOR, TGF-beta, and Wnt/beta-catenin pathway in DLBCL. Finally, results of DNA methylation analysis indicated that hypomethylation in KIF23's promoter region might be the result of its higher expression in DLBCL.
The findings of this study suggested that KIF23 is a potential biomarker for the diagnosis and prognosis of DLBCL. However, further studies were needed to validate these findings. |
| first_indexed | 2024-12-12T07:35:32Z |
| format | Article |
| id | doaj.art-a812ca7c8a664118ae78ec8240657c94 |
| institution | Directory Open Access Journal |
| issn | 0025-7974 1536-5964 |
| language | English |
| last_indexed | 2024-12-12T07:35:32Z |
| publishDate | 2022-06-01 |
| publisher | Wolters Kluwer |
| record_format | Article |
| series | Medicine |
| spelling | doaj.art-a812ca7c8a664118ae78ec8240657c942022-12-22T00:32:55ZengWolters KluwerMedicine0025-79741536-59642022-06-0110124e2931210.1097/MD.0000000000029312202206170-00016KIF23 is a potential biomarker of diffuse large B cell lymphomaYuqi Gong, PhDLingna Zhou, MDLiya Ding, MDJing Zhao, MDZhe Wang, PhDGuoping Ren, PhDJing Zhang, PhDZhengrong Mao, PhDRen Zhou, PhDAbstract. Diffuse Large B Cell Lymphoma (DLBCL), the most common form of blood cancer. The genetic and clinical heterogeneity of DLBCL poses a major barrier to diagnosis and treatment. Hence, we aim to identify potential biomarkers for DLBCL. Differentially expressed genes were screened between DLBCL and the corresponding normal tissues. Kyoto Encyclopedia of Genes and Genomes and Gene oncology analyses were performed to obtain an insight into these differentially expressed genes. PPI network was constructed to identify hub genes. survival analysis was applied to evaluate the prognostic value of those hub genes. DNA methylation analysis was implemented to explore the epigenetic dysregulation of genes in DLBCL. In this study, Kinesin family member 23 (KIF23) showed higher expression in DLBCL and was identified as a risk factor in DLBCL. The immunohistochemistry experiment further confirmed this finding. Subsequently, the univariate and multivariate analysis indicated that KIF23 might be an independent adverse factor in DLBCL. Upregulation of KIF23 might be a risk factor for the overall survival of patients who received an R-CHOP regimen, in late-stage, whatever with or without extranodal sites. Higher expression of KIF23 also significantly reduced 3, 5, 10-year overall survival. Furthermore, functional enrichment analyses (Kyoto Encyclopedia of Genes and Genomes, Gene oncology, and Gene Set Enrichment Analysis) showed that KIF23 was mainly involved in cell cycle, nuclear division, PI3K/AKT/mTOR, TGF-beta, and Wnt/beta-catenin pathway in DLBCL. Finally, results of DNA methylation analysis indicated that hypomethylation in KIF23's promoter region might be the result of its higher expression in DLBCL. The findings of this study suggested that KIF23 is a potential biomarker for the diagnosis and prognosis of DLBCL. However, further studies were needed to validate these findings.http://journals.lww.com/10.1097/MD.0000000000029312 |
| spellingShingle | Yuqi Gong, PhD Lingna Zhou, MD Liya Ding, MD Jing Zhao, MD Zhe Wang, PhD Guoping Ren, PhD Jing Zhang, PhD Zhengrong Mao, PhD Ren Zhou, PhD KIF23 is a potential biomarker of diffuse large B cell lymphoma Medicine |
| title | KIF23 is a potential biomarker of diffuse large B cell lymphoma |
| title_full | KIF23 is a potential biomarker of diffuse large B cell lymphoma |
| title_fullStr | KIF23 is a potential biomarker of diffuse large B cell lymphoma |
| title_full_unstemmed | KIF23 is a potential biomarker of diffuse large B cell lymphoma |
| title_short | KIF23 is a potential biomarker of diffuse large B cell lymphoma |
| title_sort | kif23 is a potential biomarker of diffuse large b cell lymphoma |
| url | http://journals.lww.com/10.1097/MD.0000000000029312 |
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