Growth hormone inhibits the JAK/STAT3 pathway by regulating SOCS1 in endometrial cells <em>in vitro</em>: a clue to enhance endometrial receptivity in recurrent implantation failure
Recurrent implantation failure (RIF) is defined as failure to achieve clinical pregnancy after at least 3 transfers of good-quality embryos by natural or artificial means. RIF is often a complex problem with a wide variety of etiologies and mechanisms as well as treatment options. In this study, us...
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| Format: | Article |
| Language: | English |
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PAGEPress Publications
2022-12-01
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| Series: | European Journal of Histochemistry |
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| Online Access: | https://ejh.it/index.php/ejh/article/view/3580 |
| _version_ | 1797979119500132352 |
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| author | Haixia Li Ning Sun Yaqiao Zhu Wei Wang Meihong Cai Xiaohuan Luo Wei Xia Song Quan |
| author_facet | Haixia Li Ning Sun Yaqiao Zhu Wei Wang Meihong Cai Xiaohuan Luo Wei Xia Song Quan |
| author_sort | Haixia Li |
| collection | DOAJ |
| description |
Recurrent implantation failure (RIF) is defined as failure to achieve clinical pregnancy after at least 3 transfers of good-quality embryos by natural or artificial means. RIF is often a complex problem with a wide variety of etiologies and mechanisms as well as treatment options. In this study, using immunohistochemistry and Western blot, we demonstrated that the expression of leukemia inhibitory factor (LIF), Janus kinase 1 (JAK1), and signal transducer and activator of transcription 3 (STAT3) was increased, while that of suppressor of cytokine signaling 1 (SOCS1) was decreased in RIF patients. Growth hormone (GH) administration proved to have positive effects on embryo implantation in RIF patients, but the action mechanism of GH has not been elucidated yet. To this aim, we studied the effects of GH on the proliferation in vitro of endometrial adenocarcinoma Ishikawa cells. GH stimulated the expression of LIF and SOCS1, and through SOCS1 inhibits the expression of phosphorylated STAT3, and finally inhibits the occurrence of RIF. Excessive phosphorylation of STAT can lead to decreased endometrial receptivity and abnormal embryo implantation. We also examined the effects of LIF overexpression and an LIF inhibitor (EC330) on the JAK/STAT pathway. LIF promoted cell proliferation, and the up-regulation of LIF increased the expression of SOCS1 and JAK1/STAT3 pathway-related genes in Ishikawa cells. As GH can inhibit the JAK1/STAT3 pathway through LIF, we hypothesize that upregulating SOCS1 may be a potential approach to treat RIF at the molecular level. GH can inhibit the JAK1/STAT3 pathway through LIF, up-regulating SOCS1 to treat RIF at the molecular level.
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| first_indexed | 2024-04-11T05:34:59Z |
| format | Article |
| id | doaj.art-a814ca132ecb4e8790d3a3cc1833e8ad |
| institution | Directory Open Access Journal |
| issn | 1121-760X 2038-8306 |
| language | English |
| last_indexed | 2024-04-11T05:34:59Z |
| publishDate | 2022-12-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | European Journal of Histochemistry |
| spelling | doaj.art-a814ca132ecb4e8790d3a3cc1833e8ad2022-12-22T14:52:45ZengPAGEPress PublicationsEuropean Journal of Histochemistry1121-760X2038-83062022-12-0167110.4081/ejh.2023.3580Growth hormone inhibits the JAK/STAT3 pathway by regulating SOCS1 in endometrial cells <em>in vitro</em>: a clue to enhance endometrial receptivity in recurrent implantation failureHaixia Li0Ning Sun1Yaqiao Zhu2Wei Wang3Meihong Cai4Xiaohuan Luo5Wei Xia6Song Quan7Center for Reproductive Medicine, Department of Gynecology and Obstetrics, Nanfang Hospital, Southern Medical University, GuangzhouDepartment of Reproductive Medicine Centre, Guangzhou First People's Hospital, South China University of Technology, GuangzhouDepartment of Reproductive Medicine Centre, Guangzhou First People's Hospital, South China University of Technology, GuangzhouDepartment of Reproductive Medicine Centre, Guangzhou First People's Hospital, South China University of Technology, GuangzhouDepartment of Reproductive Medicine Centre, Guangzhou First People's Hospital, South China University of Technology, GuangzhouDepartment of Reproductive Medicine Centre, Guangzhou First People's Hospital, South China University of Technology, GuangzhouDepartment of Reproductive Medicine Centre, Guangzhou First People's Hospital, South China University of Technology, GuangzhouCenter for Reproductive Medicine, Department of Gynecology and Obstetrics, Nanfang Hospital, Southern Medical University, Guangzhou Recurrent implantation failure (RIF) is defined as failure to achieve clinical pregnancy after at least 3 transfers of good-quality embryos by natural or artificial means. RIF is often a complex problem with a wide variety of etiologies and mechanisms as well as treatment options. In this study, using immunohistochemistry and Western blot, we demonstrated that the expression of leukemia inhibitory factor (LIF), Janus kinase 1 (JAK1), and signal transducer and activator of transcription 3 (STAT3) was increased, while that of suppressor of cytokine signaling 1 (SOCS1) was decreased in RIF patients. Growth hormone (GH) administration proved to have positive effects on embryo implantation in RIF patients, but the action mechanism of GH has not been elucidated yet. To this aim, we studied the effects of GH on the proliferation in vitro of endometrial adenocarcinoma Ishikawa cells. GH stimulated the expression of LIF and SOCS1, and through SOCS1 inhibits the expression of phosphorylated STAT3, and finally inhibits the occurrence of RIF. Excessive phosphorylation of STAT can lead to decreased endometrial receptivity and abnormal embryo implantation. We also examined the effects of LIF overexpression and an LIF inhibitor (EC330) on the JAK/STAT pathway. LIF promoted cell proliferation, and the up-regulation of LIF increased the expression of SOCS1 and JAK1/STAT3 pathway-related genes in Ishikawa cells. As GH can inhibit the JAK1/STAT3 pathway through LIF, we hypothesize that upregulating SOCS1 may be a potential approach to treat RIF at the molecular level. GH can inhibit the JAK1/STAT3 pathway through LIF, up-regulating SOCS1 to treat RIF at the molecular level. https://ejh.it/index.php/ejh/article/view/3580Recurrent implantation failureJAK/STAT pathwayleukemia inhibitory factorgrowth hormoneSOCS1 |
| spellingShingle | Haixia Li Ning Sun Yaqiao Zhu Wei Wang Meihong Cai Xiaohuan Luo Wei Xia Song Quan Growth hormone inhibits the JAK/STAT3 pathway by regulating SOCS1 in endometrial cells <em>in vitro</em>: a clue to enhance endometrial receptivity in recurrent implantation failure European Journal of Histochemistry Recurrent implantation failure JAK/STAT pathway leukemia inhibitory factor growth hormone SOCS1 |
| title | Growth hormone inhibits the JAK/STAT3 pathway by regulating SOCS1 in endometrial cells <em>in vitro</em>: a clue to enhance endometrial receptivity in recurrent implantation failure |
| title_full | Growth hormone inhibits the JAK/STAT3 pathway by regulating SOCS1 in endometrial cells <em>in vitro</em>: a clue to enhance endometrial receptivity in recurrent implantation failure |
| title_fullStr | Growth hormone inhibits the JAK/STAT3 pathway by regulating SOCS1 in endometrial cells <em>in vitro</em>: a clue to enhance endometrial receptivity in recurrent implantation failure |
| title_full_unstemmed | Growth hormone inhibits the JAK/STAT3 pathway by regulating SOCS1 in endometrial cells <em>in vitro</em>: a clue to enhance endometrial receptivity in recurrent implantation failure |
| title_short | Growth hormone inhibits the JAK/STAT3 pathway by regulating SOCS1 in endometrial cells <em>in vitro</em>: a clue to enhance endometrial receptivity in recurrent implantation failure |
| title_sort | growth hormone inhibits the jak stat3 pathway by regulating socs1 in endometrial cells em in vitro em a clue to enhance endometrial receptivity in recurrent implantation failure |
| topic | Recurrent implantation failure JAK/STAT pathway leukemia inhibitory factor growth hormone SOCS1 |
| url | https://ejh.it/index.php/ejh/article/view/3580 |
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