Evaluation of ionophore resistance in field isolates of Eimeria tenella from Jammu and Kashmir
The present study was conducted to evaluate resistance against ionophores (salinomycin and maduramicin) in four field isolates (F) of Eimeria tenella collected from four distantly located poultry farms from Union territory of Jammu and Kashmir. The purity of isolates was ascertained by COCCIMORPH a...
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| Format: | Article |
| Language: | English |
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Indian Council of Agricultural Research
2023-09-01
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| Series: | Indian Journal of Animal Sciences |
| Subjects: | |
| Online Access: | https://epubs.icar.org.in/index.php/IJAnS/article/view/132460 |
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| author | AIMAN KHURSHEED ANISH YADAV VIKAS YADAV OMER M SOFI ANAND KUSHWAHA SHAFIYA I RAFIQI RAJESH GODARA SHILPA SOOD DIBYENDU CHAKRABORTY RAJESH KATOCH |
| author_facet | AIMAN KHURSHEED ANISH YADAV VIKAS YADAV OMER M SOFI ANAND KUSHWAHA SHAFIYA I RAFIQI RAJESH GODARA SHILPA SOOD DIBYENDU CHAKRABORTY RAJESH KATOCH |
| author_sort | AIMAN KHURSHEED |
| collection | DOAJ |
| description |
The present study was conducted to evaluate resistance against ionophores (salinomycin and maduramicin) in four field isolates (F) of Eimeria tenella collected from four distantly located poultry farms from Union territory of Jammu and Kashmir. The purity of isolates was ascertained by COCCIMORPH and ITS-1 gene amplification for E. tenella. For the drug sensitivity trial, Cobb strain, day-old male broiler chicks were used. At the age of 10 days, the birds were transferred ad random in 13 groups to battery cage system. All birds, except non infected non-medicated control were infected with 105 sporulated oocysts each at two weeks. Prophylactic medication with ionophores commenced two days prior to infection in eight groups (two medicated groups for each of the four isolates). Another four groups served as infected and non-medicated control. Seven days post infection, all the surviving birds were sacrificed to study drug resistance by assessing the global index for individual ionophore compounds. The F-1 exhibited resistance against salinomycin and maduramicin whereas, the F-2 showed comparatively improved global index for both salinomycin and maduramicin thus indicating limited efficacy. The F-3 exhibited partial resistance against salinomycin and limited efficacy in case of maduaramicin, and the F-4 was observed to be resistant against salinomycin whereas partially resistant against maduramicin. The present study provides evidence of resistance against maduramicin and salinomycin in field isolates of E. tenella from Union Territory of Jammu and Kashmir which may aid in management of poultry coccidiosis.
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| first_indexed | 2024-03-12T01:16:54Z |
| format | Article |
| id | doaj.art-a81780e3d59c461ca26bd3ca79a538c9 |
| institution | Directory Open Access Journal |
| issn | 0367-8318 2394-3327 |
| language | English |
| last_indexed | 2024-03-12T01:16:54Z |
| publishDate | 2023-09-01 |
| publisher | Indian Council of Agricultural Research |
| record_format | Article |
| series | Indian Journal of Animal Sciences |
| spelling | doaj.art-a81780e3d59c461ca26bd3ca79a538c92023-09-13T11:20:27ZengIndian Council of Agricultural ResearchIndian Journal of Animal Sciences0367-83182394-33272023-09-0193910.56093/ijans.v93i9.132460Evaluation of ionophore resistance in field isolates of Eimeria tenella from Jammu and KashmirAIMAN KHURSHEED0ANISH YADAV1VIKAS YADAV2OMER M SOFI3ANAND KUSHWAHA4SHAFIYA I RAFIQI5RAJESH GODARA6SHILPA SOOD7DIBYENDU CHAKRABORTY8RAJESH KATOCH9Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, Jammu, Jammu and Kashmir 181 102 IndiaSher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, Jammu, Jammu and Kashmir 181 102 IndiaSher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, Jammu, Jammu and Kashmir 181 102 IndiaSher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, Jammu, Jammu and Kashmir 181 102 IndiaSher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, Jammu, Jammu and Kashmir 181 102 IndiaSher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, Jammu, Jammu and Kashmir 181 102 IndiaSher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, Jammu, Jammu and Kashmir 181 102 IndiaSher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, Jammu, Jammu and Kashmir 181 102 IndiaSher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, Jammu, Jammu and Kashmir 181 102 IndiaSher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, Jammu, Jammu and Kashmir 181 102 India The present study was conducted to evaluate resistance against ionophores (salinomycin and maduramicin) in four field isolates (F) of Eimeria tenella collected from four distantly located poultry farms from Union territory of Jammu and Kashmir. The purity of isolates was ascertained by COCCIMORPH and ITS-1 gene amplification for E. tenella. For the drug sensitivity trial, Cobb strain, day-old male broiler chicks were used. At the age of 10 days, the birds were transferred ad random in 13 groups to battery cage system. All birds, except non infected non-medicated control were infected with 105 sporulated oocysts each at two weeks. Prophylactic medication with ionophores commenced two days prior to infection in eight groups (two medicated groups for each of the four isolates). Another four groups served as infected and non-medicated control. Seven days post infection, all the surviving birds were sacrificed to study drug resistance by assessing the global index for individual ionophore compounds. The F-1 exhibited resistance against salinomycin and maduramicin whereas, the F-2 showed comparatively improved global index for both salinomycin and maduramicin thus indicating limited efficacy. The F-3 exhibited partial resistance against salinomycin and limited efficacy in case of maduaramicin, and the F-4 was observed to be resistant against salinomycin whereas partially resistant against maduramicin. The present study provides evidence of resistance against maduramicin and salinomycin in field isolates of E. tenella from Union Territory of Jammu and Kashmir which may aid in management of poultry coccidiosis. https://epubs.icar.org.in/index.php/IJAnS/article/view/132460Anticoccidial, Eimeria tenella, India, Ionophores, Resistance |
| spellingShingle | AIMAN KHURSHEED ANISH YADAV VIKAS YADAV OMER M SOFI ANAND KUSHWAHA SHAFIYA I RAFIQI RAJESH GODARA SHILPA SOOD DIBYENDU CHAKRABORTY RAJESH KATOCH Evaluation of ionophore resistance in field isolates of Eimeria tenella from Jammu and Kashmir Indian Journal of Animal Sciences Anticoccidial, Eimeria tenella, India, Ionophores, Resistance |
| title | Evaluation of ionophore resistance in field isolates of Eimeria tenella from Jammu and Kashmir |
| title_full | Evaluation of ionophore resistance in field isolates of Eimeria tenella from Jammu and Kashmir |
| title_fullStr | Evaluation of ionophore resistance in field isolates of Eimeria tenella from Jammu and Kashmir |
| title_full_unstemmed | Evaluation of ionophore resistance in field isolates of Eimeria tenella from Jammu and Kashmir |
| title_short | Evaluation of ionophore resistance in field isolates of Eimeria tenella from Jammu and Kashmir |
| title_sort | evaluation of ionophore resistance in field isolates of eimeria tenella from jammu and kashmir |
| topic | Anticoccidial, Eimeria tenella, India, Ionophores, Resistance |
| url | https://epubs.icar.org.in/index.php/IJAnS/article/view/132460 |
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