Identification and Characterization of <i>WRKY41</i>, a Gene Conferring Resistance to Powdery Mildew in Wild Tomato (<i>Solanum habrochaites</i>) LA1777
WRKYs, a large family of transcription factors, are involved in plant response to biotic and abiotic stresses, but the role of them in tomato resistance to <i>Oidium neolycopersici</i> is still unclear. In this study, we evaluate the role of <i>WRKYs</i> in powdery mildew-res...
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MDPI AG
2022-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/3/1267 |
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| author | Qinggui Lian Xinyi He Bingbing Zhang Yang Wang Qing Ma |
| author_facet | Qinggui Lian Xinyi He Bingbing Zhang Yang Wang Qing Ma |
| author_sort | Qinggui Lian |
| collection | DOAJ |
| description | WRKYs, a large family of transcription factors, are involved in plant response to biotic and abiotic stresses, but the role of them in tomato resistance to <i>Oidium neolycopersici</i> is still unclear. In this study, we evaluate the role of <i>WRKYs</i> in powdery mildew-resistant wild tomato (<i>Solanum habrochaites</i>) LA1777 defense against <i>O. neolycopersici</i> strain lz (<i>On</i>-lz) using a combination of omics, classical plant pathology- and cell biology-based approaches. A total of 27 <i>WRKYs</i>, belonging to group I, II, and III, were identified as differentially expressed genes in LA1777 against <i>On</i>-lz. It was found that expression of <i>ShWRKY41</i> was increased after <i>Pseudomonas syringae</i> pv. tomato (<i>Pst</i>) DC3000, <i>On</i>-lz and <i>Botrytis</i><i>cinerea</i> B05 inoculation or ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC) treatment. GUS staining of <i>ShWRKY41</i> promoter indicated that the expression of <i>ShWRKY41</i> could be induced by SA and ethylene. Furthermore, <i>ShWRKY41</i> gene silencing reduced the resistance to <i>On</i>-lz infection by decreasing the generation of H<sub>2</sub>O<sub>2</sub> and HR in LA1777 seedlings. Overall, our research suggests that <i>ShWRKY41</i> plays a positive role in defense activation and host resistance to <i>O. neolycopersici</i> in wild tomato (<i>S. habrochaites</i>) LA1777. |
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| language | English |
| last_indexed | 2024-03-09T23:49:52Z |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-a81d18b43857485bb355dc6a634d5a9d2023-11-23T16:37:39ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-01-01233126710.3390/ijms23031267Identification and Characterization of <i>WRKY41</i>, a Gene Conferring Resistance to Powdery Mildew in Wild Tomato (<i>Solanum habrochaites</i>) LA1777Qinggui Lian0Xinyi He1Bingbing Zhang2Yang Wang3Qing Ma4College of Plant Protection, Northwest A&F University, Xianyang 712100, ChinaCollege of Plant Protection, Northwest A&F University, Xianyang 712100, ChinaCollege of Plant Protection, Northwest A&F University, Xianyang 712100, ChinaCollege of Plant Protection, Northwest A&F University, Xianyang 712100, ChinaCollege of Plant Protection, Northwest A&F University, Xianyang 712100, ChinaWRKYs, a large family of transcription factors, are involved in plant response to biotic and abiotic stresses, but the role of them in tomato resistance to <i>Oidium neolycopersici</i> is still unclear. In this study, we evaluate the role of <i>WRKYs</i> in powdery mildew-resistant wild tomato (<i>Solanum habrochaites</i>) LA1777 defense against <i>O. neolycopersici</i> strain lz (<i>On</i>-lz) using a combination of omics, classical plant pathology- and cell biology-based approaches. A total of 27 <i>WRKYs</i>, belonging to group I, II, and III, were identified as differentially expressed genes in LA1777 against <i>On</i>-lz. It was found that expression of <i>ShWRKY41</i> was increased after <i>Pseudomonas syringae</i> pv. tomato (<i>Pst</i>) DC3000, <i>On</i>-lz and <i>Botrytis</i><i>cinerea</i> B05 inoculation or ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC) treatment. GUS staining of <i>ShWRKY41</i> promoter indicated that the expression of <i>ShWRKY41</i> could be induced by SA and ethylene. Furthermore, <i>ShWRKY41</i> gene silencing reduced the resistance to <i>On</i>-lz infection by decreasing the generation of H<sub>2</sub>O<sub>2</sub> and HR in LA1777 seedlings. Overall, our research suggests that <i>ShWRKY41</i> plays a positive role in defense activation and host resistance to <i>O. neolycopersici</i> in wild tomato (<i>S. habrochaites</i>) LA1777.https://www.mdpi.com/1422-0067/23/3/1267tomato powdery mildew<i>Oidium neolycopersici</i><i>WRKYs</i>resistance |
| spellingShingle | Qinggui Lian Xinyi He Bingbing Zhang Yang Wang Qing Ma Identification and Characterization of <i>WRKY41</i>, a Gene Conferring Resistance to Powdery Mildew in Wild Tomato (<i>Solanum habrochaites</i>) LA1777 International Journal of Molecular Sciences tomato powdery mildew <i>Oidium neolycopersici</i> <i>WRKYs</i> resistance |
| title | Identification and Characterization of <i>WRKY41</i>, a Gene Conferring Resistance to Powdery Mildew in Wild Tomato (<i>Solanum habrochaites</i>) LA1777 |
| title_full | Identification and Characterization of <i>WRKY41</i>, a Gene Conferring Resistance to Powdery Mildew in Wild Tomato (<i>Solanum habrochaites</i>) LA1777 |
| title_fullStr | Identification and Characterization of <i>WRKY41</i>, a Gene Conferring Resistance to Powdery Mildew in Wild Tomato (<i>Solanum habrochaites</i>) LA1777 |
| title_full_unstemmed | Identification and Characterization of <i>WRKY41</i>, a Gene Conferring Resistance to Powdery Mildew in Wild Tomato (<i>Solanum habrochaites</i>) LA1777 |
| title_short | Identification and Characterization of <i>WRKY41</i>, a Gene Conferring Resistance to Powdery Mildew in Wild Tomato (<i>Solanum habrochaites</i>) LA1777 |
| title_sort | identification and characterization of i wrky41 i a gene conferring resistance to powdery mildew in wild tomato i solanum habrochaites i la1777 |
| topic | tomato powdery mildew <i>Oidium neolycopersici</i> <i>WRKYs</i> resistance |
| url | https://www.mdpi.com/1422-0067/23/3/1267 |
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