Investigation of Potential Effects of Ibuprofen on the Storage Cells and Anhydrobiosis Capacity of the Tardigrade <i>Paramacrobiotus experimentalis</i>
The surge in pharmaceutical consumption, particularly non-steroidal anti-inflammatory drugs (NSAIDs) such as ibuprofen, has raised concerns about their presence in aquatic ecosystems. This study investigated the potential ecological impact of ibuprofen, focusing on the ultrastructure of storage cell...
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| פורמט: | Article |
| שפה: | English |
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MDPI AG
2024-02-01
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| סדרה: | Diversity |
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| גישה מקוונת: | https://www.mdpi.com/1424-2818/16/3/132 |
| _version_ | 1827306511286665216 |
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| author | Aleksandra Miernik Filip Wieczorkiewicz Sebastian Student Izabela Poprawa |
| author_facet | Aleksandra Miernik Filip Wieczorkiewicz Sebastian Student Izabela Poprawa |
| author_sort | Aleksandra Miernik |
| collection | DOAJ |
| description | The surge in pharmaceutical consumption, particularly non-steroidal anti-inflammatory drugs (NSAIDs) such as ibuprofen, has raised concerns about their presence in aquatic ecosystems. This study investigated the potential ecological impact of ibuprofen, focusing on the ultrastructure of storage cells in the tardigrade <i>Paramacrobiotus experimentalis</i>, renowned for its resilience to environmental stressors. Individuals were exposed to three ibuprofen concentrations (0.1 μg/L, 16.8 μg/L, and 1 mg/L) over 7 and 28 days. Storage cells were examined using light microscopy, transmission electron microscopy, and confocal microscopy. This study also explored ibuprofen’s impact on the process of anhydrobiosis. In the short-term experiment, no ultrastructural changes in tardigrade storage cells were observed across ibuprofen concentrations. However, in the long-term incubation, autophagic structures in storage cell cytoplasm were identified, indicating potential adaptive responses. Individual mitochondria exhibited degeneration, and the rough endoplasmic reticulum displayed slight swelling. No evidence of increased oxidative stress or nuclear DNA fragmentation was observed in any research group. This study elucidates the complex responses of tardigrade storage cells to ibuprofen exposure. The findings emphasize the importance of understanding pharmaceutical impacts on aquatic organisms, highlighting the resilience of tardigrades to specific environmental stressors. |
| first_indexed | 2024-04-24T18:23:46Z |
| format | Article |
| id | doaj.art-a81da873f2b044d991d4a8f14ea84b47 |
| institution | Directory Open Access Journal |
| issn | 1424-2818 |
| language | English |
| last_indexed | 2024-04-24T18:23:46Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diversity |
| spelling | doaj.art-a81da873f2b044d991d4a8f14ea84b472024-03-27T13:33:42ZengMDPI AGDiversity1424-28182024-02-0116313210.3390/d16030132Investigation of Potential Effects of Ibuprofen on the Storage Cells and Anhydrobiosis Capacity of the Tardigrade <i>Paramacrobiotus experimentalis</i>Aleksandra Miernik0Filip Wieczorkiewicz1Sebastian Student2Izabela Poprawa3Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice, Bankowa 9, 40-007 Katowice, PolandInstitute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice, Bankowa 9, 40-007 Katowice, PolandInstitute of Automatic Control, Silesian University of Technology, Akademicka 16, 44-100 Gliwice, PolandInstitute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice, Bankowa 9, 40-007 Katowice, PolandThe surge in pharmaceutical consumption, particularly non-steroidal anti-inflammatory drugs (NSAIDs) such as ibuprofen, has raised concerns about their presence in aquatic ecosystems. This study investigated the potential ecological impact of ibuprofen, focusing on the ultrastructure of storage cells in the tardigrade <i>Paramacrobiotus experimentalis</i>, renowned for its resilience to environmental stressors. Individuals were exposed to three ibuprofen concentrations (0.1 μg/L, 16.8 μg/L, and 1 mg/L) over 7 and 28 days. Storage cells were examined using light microscopy, transmission electron microscopy, and confocal microscopy. This study also explored ibuprofen’s impact on the process of anhydrobiosis. In the short-term experiment, no ultrastructural changes in tardigrade storage cells were observed across ibuprofen concentrations. However, in the long-term incubation, autophagic structures in storage cell cytoplasm were identified, indicating potential adaptive responses. Individual mitochondria exhibited degeneration, and the rough endoplasmic reticulum displayed slight swelling. No evidence of increased oxidative stress or nuclear DNA fragmentation was observed in any research group. This study elucidates the complex responses of tardigrade storage cells to ibuprofen exposure. The findings emphasize the importance of understanding pharmaceutical impacts on aquatic organisms, highlighting the resilience of tardigrades to specific environmental stressors.https://www.mdpi.com/1424-2818/16/3/132tardigradesanhydrobiosisibuprofen |
| spellingShingle | Aleksandra Miernik Filip Wieczorkiewicz Sebastian Student Izabela Poprawa Investigation of Potential Effects of Ibuprofen on the Storage Cells and Anhydrobiosis Capacity of the Tardigrade <i>Paramacrobiotus experimentalis</i> Diversity tardigrades anhydrobiosis ibuprofen |
| title | Investigation of Potential Effects of Ibuprofen on the Storage Cells and Anhydrobiosis Capacity of the Tardigrade <i>Paramacrobiotus experimentalis</i> |
| title_full | Investigation of Potential Effects of Ibuprofen on the Storage Cells and Anhydrobiosis Capacity of the Tardigrade <i>Paramacrobiotus experimentalis</i> |
| title_fullStr | Investigation of Potential Effects of Ibuprofen on the Storage Cells and Anhydrobiosis Capacity of the Tardigrade <i>Paramacrobiotus experimentalis</i> |
| title_full_unstemmed | Investigation of Potential Effects of Ibuprofen on the Storage Cells and Anhydrobiosis Capacity of the Tardigrade <i>Paramacrobiotus experimentalis</i> |
| title_short | Investigation of Potential Effects of Ibuprofen on the Storage Cells and Anhydrobiosis Capacity of the Tardigrade <i>Paramacrobiotus experimentalis</i> |
| title_sort | investigation of potential effects of ibuprofen on the storage cells and anhydrobiosis capacity of the tardigrade i paramacrobiotus experimentalis i |
| topic | tardigrades anhydrobiosis ibuprofen |
| url | https://www.mdpi.com/1424-2818/16/3/132 |
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