Nobiletin promotes the pyroptosis of breast cancer via regulation of miR‐200b/JAZF1 axis
Abstract Nobiletin is a polymethoxylated flavone present in citrus fruits, which has been reported to have inhibitory effects on tumorigenesis of cancers. However, the biological function of nobiletin in breast cancer (BC) is largely unknown. To investigate the effect of nobiletin on growth of BC ce...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2021-07-01
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| Series: | Kaohsiung Journal of Medical Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/kjm2.12371 |
| _version_ | 1818439885302792192 |
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| author | Ji‐Guo Wang Wen‐Jing Jian Yang Li Jing Zhang |
| author_facet | Ji‐Guo Wang Wen‐Jing Jian Yang Li Jing Zhang |
| author_sort | Ji‐Guo Wang |
| collection | DOAJ |
| description | Abstract Nobiletin is a polymethoxylated flavone present in citrus fruits, which has been reported to have inhibitory effects on tumorigenesis of cancers. However, the biological function of nobiletin in breast cancer (BC) is largely unknown. To investigate the effect of nobiletin on growth of BC cells, the cell viability of BC was measured by MTT assay. In addition, gene and protein expressions were detected by qRT‐PCR and western blot, respectively. The apoptosis and pyroptosis of BC cells were tested by flow cytometry. Finally, the correlation between miR‐200b and JAZF1 was detected by dual luciferase report. The data indicated that nobiletin inhibited the proliferation of BC cells in a dose‐dependent manner. Moreover, miR‐200b mimics‐induced pyroptosis of BC cells was further increased by nobiletin. Meanwhile, JAZF1 was found to be the target of miR‐200b. Moreover, nobiletin induced apoptosis and pyroptosis of BC cells via miR‐200b/JAZF1/NF‐κB axis. In conclusion, nobiletin inhibited the tumorigenesis of BC via regulation of miR‐200b/JAZF1 axis. Thus, nobiletin might serve as a new agent for the treatment of BC. |
| first_indexed | 2024-12-14T18:03:34Z |
| format | Article |
| id | doaj.art-a81fa21035544d74ae95730040c443ba |
| institution | Directory Open Access Journal |
| issn | 1607-551X 2410-8650 |
| language | English |
| last_indexed | 2024-12-14T18:03:34Z |
| publishDate | 2021-07-01 |
| publisher | Wiley |
| record_format | Article |
| series | Kaohsiung Journal of Medical Sciences |
| spelling | doaj.art-a81fa21035544d74ae95730040c443ba2022-12-21T22:52:24ZengWileyKaohsiung Journal of Medical Sciences1607-551X2410-86502021-07-0137757258210.1002/kjm2.12371Nobiletin promotes the pyroptosis of breast cancer via regulation of miR‐200b/JAZF1 axisJi‐Guo Wang0Wen‐Jing Jian1Yang Li2Jing Zhang3Department of Oncology Shenzhen Baoan Hospital of Traditional Chinese Medicine Shenzhen ChinaDepartment of Breast Surgery The Second People's Hospital of Shenzhen Shenzhen ChinaDepartment of Oncology Shenzhen Baoan Hospital of Traditional Chinese Medicine Shenzhen ChinaDepartment of Oncology Shenzhen Baoan Hospital of Traditional Chinese Medicine Shenzhen ChinaAbstract Nobiletin is a polymethoxylated flavone present in citrus fruits, which has been reported to have inhibitory effects on tumorigenesis of cancers. However, the biological function of nobiletin in breast cancer (BC) is largely unknown. To investigate the effect of nobiletin on growth of BC cells, the cell viability of BC was measured by MTT assay. In addition, gene and protein expressions were detected by qRT‐PCR and western blot, respectively. The apoptosis and pyroptosis of BC cells were tested by flow cytometry. Finally, the correlation between miR‐200b and JAZF1 was detected by dual luciferase report. The data indicated that nobiletin inhibited the proliferation of BC cells in a dose‐dependent manner. Moreover, miR‐200b mimics‐induced pyroptosis of BC cells was further increased by nobiletin. Meanwhile, JAZF1 was found to be the target of miR‐200b. Moreover, nobiletin induced apoptosis and pyroptosis of BC cells via miR‐200b/JAZF1/NF‐κB axis. In conclusion, nobiletin inhibited the tumorigenesis of BC via regulation of miR‐200b/JAZF1 axis. Thus, nobiletin might serve as a new agent for the treatment of BC.https://doi.org/10.1002/kjm2.12371breast cancerJAZF1miR‐200bNF‐κB signaling pathway |
| spellingShingle | Ji‐Guo Wang Wen‐Jing Jian Yang Li Jing Zhang Nobiletin promotes the pyroptosis of breast cancer via regulation of miR‐200b/JAZF1 axis Kaohsiung Journal of Medical Sciences breast cancer JAZF1 miR‐200b NF‐κB signaling pathway |
| title | Nobiletin promotes the pyroptosis of breast cancer via regulation of miR‐200b/JAZF1 axis |
| title_full | Nobiletin promotes the pyroptosis of breast cancer via regulation of miR‐200b/JAZF1 axis |
| title_fullStr | Nobiletin promotes the pyroptosis of breast cancer via regulation of miR‐200b/JAZF1 axis |
| title_full_unstemmed | Nobiletin promotes the pyroptosis of breast cancer via regulation of miR‐200b/JAZF1 axis |
| title_short | Nobiletin promotes the pyroptosis of breast cancer via regulation of miR‐200b/JAZF1 axis |
| title_sort | nobiletin promotes the pyroptosis of breast cancer via regulation of mir 200b jazf1 axis |
| topic | breast cancer JAZF1 miR‐200b NF‐κB signaling pathway |
| url | https://doi.org/10.1002/kjm2.12371 |
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