THP-1 cells form foam cells in response to coculture with lipoproteins but not platelets.
The human monocytic leukemia cell line, THP-1, shares many properties with human monocyte-derived macrophages and might be a useful model for studying foam cell formation in vitro. Therefore, we examined the ability of THP-1 cells to accumulate cholesteryl esters, the hallmark feature of foam cells,...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
1991-01-01
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| Series: | Journal of Lipid Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520422412 |
| _version_ | 1818727159793975296 |
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| author | CL Banka AS Black CA Dyer LK Curtiss |
| author_facet | CL Banka AS Black CA Dyer LK Curtiss |
| author_sort | CL Banka |
| collection | DOAJ |
| description | The human monocytic leukemia cell line, THP-1, shares many properties with human monocyte-derived macrophages and might be a useful model for studying foam cell formation in vitro. Therefore, we examined the ability of THP-1 cells to accumulate cholesteryl esters, the hallmark feature of foam cells, in response to culture with native low density lipoprotein (LDL), modified LDL, and platelets. THP-1 cells stored more cholesteryl esters than macrophages in response to 200 micrograms/ml of LDL. Down-regulation of LDL receptors occurred in macrophages at lower LDL concentrations than in THP-1 cells. Phorbol ester-treated THP-1 cells stored more cholesteryl esters than human macrophages in response to 25-200 micrograms/ml of acetylated LDL. Because we have previously demonstrated that activated platelets enhanced macrophage cholesteryl ester storage, we examined the ability of THP-1 cells to store cholesteryl esters in response to coculture with platelets. Compared with macrophages, dividing THP-1 cells and phorbol ester-treated THP-1 cells accumulated only 50% and 33% as much cholesteryl esters, respectively. Furthermore, although platelets induced a 90% reduction in cholesterol synthesis in macrophages by day 5, cholesterol synthesis in THP-1 cells and phorbol ester-treated THP-1 cells was inhibited less than 50% by platelets. Nevertheless, both THP-1 cells and macrophages responded to platelets by increasing their secretion of apolipoprotein E. Therefore, we conclude that dividing THP-1 cells and phorbol ester-treated THP-1 cells are capable of forming foam cells in response to physiologic doses of both LDL and acetylated LDL, respectively.(ABSTRACT TRUNCATED AT 250 WORDS) |
| first_indexed | 2024-12-17T22:09:40Z |
| format | Article |
| id | doaj.art-a820a2905e4b42f9abb812eb9d90aef0 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-17T22:09:40Z |
| publishDate | 1991-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-a820a2905e4b42f9abb812eb9d90aef02022-12-21T21:30:47ZengElsevierJournal of Lipid Research0022-22751991-01-013213543THP-1 cells form foam cells in response to coculture with lipoproteins but not platelets.CL Banka0AS Black1CA Dyer2LK Curtiss3Department of Immunology, Research Institute of Scripps Clinic, La Jolla, CA 92037.Department of Immunology, Research Institute of Scripps Clinic, La Jolla, CA 92037.Department of Immunology, Research Institute of Scripps Clinic, La Jolla, CA 92037.Department of Immunology, Research Institute of Scripps Clinic, La Jolla, CA 92037.The human monocytic leukemia cell line, THP-1, shares many properties with human monocyte-derived macrophages and might be a useful model for studying foam cell formation in vitro. Therefore, we examined the ability of THP-1 cells to accumulate cholesteryl esters, the hallmark feature of foam cells, in response to culture with native low density lipoprotein (LDL), modified LDL, and platelets. THP-1 cells stored more cholesteryl esters than macrophages in response to 200 micrograms/ml of LDL. Down-regulation of LDL receptors occurred in macrophages at lower LDL concentrations than in THP-1 cells. Phorbol ester-treated THP-1 cells stored more cholesteryl esters than human macrophages in response to 25-200 micrograms/ml of acetylated LDL. Because we have previously demonstrated that activated platelets enhanced macrophage cholesteryl ester storage, we examined the ability of THP-1 cells to store cholesteryl esters in response to coculture with platelets. Compared with macrophages, dividing THP-1 cells and phorbol ester-treated THP-1 cells accumulated only 50% and 33% as much cholesteryl esters, respectively. Furthermore, although platelets induced a 90% reduction in cholesterol synthesis in macrophages by day 5, cholesterol synthesis in THP-1 cells and phorbol ester-treated THP-1 cells was inhibited less than 50% by platelets. Nevertheless, both THP-1 cells and macrophages responded to platelets by increasing their secretion of apolipoprotein E. Therefore, we conclude that dividing THP-1 cells and phorbol ester-treated THP-1 cells are capable of forming foam cells in response to physiologic doses of both LDL and acetylated LDL, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)http://www.sciencedirect.com/science/article/pii/S0022227520422412 |
| spellingShingle | CL Banka AS Black CA Dyer LK Curtiss THP-1 cells form foam cells in response to coculture with lipoproteins but not platelets. Journal of Lipid Research |
| title | THP-1 cells form foam cells in response to coculture with lipoproteins but not platelets. |
| title_full | THP-1 cells form foam cells in response to coculture with lipoproteins but not platelets. |
| title_fullStr | THP-1 cells form foam cells in response to coculture with lipoproteins but not platelets. |
| title_full_unstemmed | THP-1 cells form foam cells in response to coculture with lipoproteins but not platelets. |
| title_short | THP-1 cells form foam cells in response to coculture with lipoproteins but not platelets. |
| title_sort | thp 1 cells form foam cells in response to coculture with lipoproteins but not platelets |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520422412 |
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