Characterization and degradation of pectin derived from Budimka apple
The characterization of apple pectin and its oligogalacturonic fractions derived from the autochthones apple variety Budimka, characteristic for central Serbia, is described in this paper. After extraction, the apple pectin was subjected to controlled enzymatic hydrolysis by polygalacturonase (PG) a...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Serbian Chemical Society
2008-02-01
|
| Series: | Journal of the Serbian Chemical Society |
| Subjects: | |
| Online Access: | http://www.shd.org.yu/JSCS/Vol73/No2/JSCS-3698.pdf |
| _version_ | 1818758574822653952 |
|---|---|
| author | MILOS V. NIKOLIC LJILJANA MOJOVIC |
| author_facet | MILOS V. NIKOLIC LJILJANA MOJOVIC |
| author_sort | MILOS V. NIKOLIC |
| collection | DOAJ |
| description | The characterization of apple pectin and its oligogalacturonic fractions derived from the autochthones apple variety Budimka, characteristic for central Serbia, is described in this paper. After extraction, the apple pectin was subjected to controlled enzymatic hydrolysis by polygalacturonase (PG) and pectin lyase (PL) from Aspergillus niger and then fractionated by ion-exchange column chromatography on Dowex 1X-8 (200–400 mesh). Saturated oligogalacturonic acids, obtained by controlled hydrolysis with PG, were efficiently separated by elution with a gradient of Na acetate buffer (pH 6.0), while unsaturated oligogalacturonic acids, obtained by controlled hydrolysis with PL, were separated on the same resin, using a gradient of Na formate buffer (pH 4.7) as the eluent. The yields of the fractions with the particular degree of polymerization (DP) were also determined. The total content of neutral saccharides in the original Budimka apple pectin was detected by HPLC analysis of the 4-nitrobenzoyl derivatives of the sugar, and amounted to 5.31 %. Among the neutral saccharides, contents of galactose, glucose, rhamnose, arabinose, xylose and mannose were detected. |
| first_indexed | 2024-12-18T06:29:00Z |
| format | Article |
| id | doaj.art-a8226223e94a44048cd4024832cbb5cf |
| institution | Directory Open Access Journal |
| issn | 0352-5139 |
| language | English |
| last_indexed | 2024-12-18T06:29:00Z |
| publishDate | 2008-02-01 |
| publisher | Serbian Chemical Society |
| record_format | Article |
| series | Journal of the Serbian Chemical Society |
| spelling | doaj.art-a8226223e94a44048cd4024832cbb5cf2022-12-21T21:17:57ZengSerbian Chemical SocietyJournal of the Serbian Chemical Society0352-51392008-02-01732157167Characterization and degradation of pectin derived from Budimka appleMILOS V. NIKOLICLJILJANA MOJOVICThe characterization of apple pectin and its oligogalacturonic fractions derived from the autochthones apple variety Budimka, characteristic for central Serbia, is described in this paper. After extraction, the apple pectin was subjected to controlled enzymatic hydrolysis by polygalacturonase (PG) and pectin lyase (PL) from Aspergillus niger and then fractionated by ion-exchange column chromatography on Dowex 1X-8 (200–400 mesh). Saturated oligogalacturonic acids, obtained by controlled hydrolysis with PG, were efficiently separated by elution with a gradient of Na acetate buffer (pH 6.0), while unsaturated oligogalacturonic acids, obtained by controlled hydrolysis with PL, were separated on the same resin, using a gradient of Na formate buffer (pH 4.7) as the eluent. The yields of the fractions with the particular degree of polymerization (DP) were also determined. The total content of neutral saccharides in the original Budimka apple pectin was detected by HPLC analysis of the 4-nitrobenzoyl derivatives of the sugar, and amounted to 5.31 %. Among the neutral saccharides, contents of galactose, glucose, rhamnose, arabinose, xylose and mannose were detected.http://www.shd.org.yu/JSCS/Vol73/No2/JSCS-3698.pdfsynergistic degradationfractionationoligogalacturonic acidsBudimka apple pectinpolygalacturonasepectin lyaseneutral saccharides |
| spellingShingle | MILOS V. NIKOLIC LJILJANA MOJOVIC Characterization and degradation of pectin derived from Budimka apple Journal of the Serbian Chemical Society synergistic degradation fractionation oligogalacturonic acids Budimka apple pectin polygalacturonase pectin lyase neutral saccharides |
| title | Characterization and degradation of pectin derived from Budimka apple |
| title_full | Characterization and degradation of pectin derived from Budimka apple |
| title_fullStr | Characterization and degradation of pectin derived from Budimka apple |
| title_full_unstemmed | Characterization and degradation of pectin derived from Budimka apple |
| title_short | Characterization and degradation of pectin derived from Budimka apple |
| title_sort | characterization and degradation of pectin derived from budimka apple |
| topic | synergistic degradation fractionation oligogalacturonic acids Budimka apple pectin polygalacturonase pectin lyase neutral saccharides |
| url | http://www.shd.org.yu/JSCS/Vol73/No2/JSCS-3698.pdf |
| work_keys_str_mv | AT milosvnikolic characterizationanddegradationofpectinderivedfrombudimkaapple AT ljiljanamojovic characterizationanddegradationofpectinderivedfrombudimkaapple |