Genomic Evidence for Cryptic Speciation in Tree Frogs From the Apennine Peninsula, With Description of Hyla perrini sp. nov
Despite increasing appreciation of the speciation continuum, delimiting and describing new species is a major yet necessary challenge of modern phylogeography to help optimize conservation efforts. In amphibians, the lack of phenotypic differences between closely-related taxa, their complex, sometim...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-10-01
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| Series: | Frontiers in Ecology and Evolution |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fevo.2018.00144/full |
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| author | Christophe Dufresnes Christophe Dufresnes Christophe Dufresnes Glib Mazepa Glib Mazepa Nicolas Rodrigues Alan Brelsford Alan Brelsford Spartak N. Litvinchuk Roberto Sermier Guillaume Lavanchy Caroline Betto-Colliard Olivier Blaser Amaël Borzée Elisa Cavoto Guillaume Fabre Karim Ghali Christine Grossen Agnes Horn Julien Leuenberger Barret C. Phillips Paul A. Saunders Romain Savary Tiziano Maddalena Matthias Stöck Sylvain Dubey Sylvain Dubey Daniele Canestrelli Daniel L. Jeffries |
| author_facet | Christophe Dufresnes Christophe Dufresnes Christophe Dufresnes Glib Mazepa Glib Mazepa Nicolas Rodrigues Alan Brelsford Alan Brelsford Spartak N. Litvinchuk Roberto Sermier Guillaume Lavanchy Caroline Betto-Colliard Olivier Blaser Amaël Borzée Elisa Cavoto Guillaume Fabre Karim Ghali Christine Grossen Agnes Horn Julien Leuenberger Barret C. Phillips Paul A. Saunders Romain Savary Tiziano Maddalena Matthias Stöck Sylvain Dubey Sylvain Dubey Daniele Canestrelli Daniel L. Jeffries |
| author_sort | Christophe Dufresnes |
| collection | DOAJ |
| description | Despite increasing appreciation of the speciation continuum, delimiting and describing new species is a major yet necessary challenge of modern phylogeography to help optimize conservation efforts. In amphibians, the lack of phenotypic differences between closely-related taxa, their complex, sometimes unresolved phylogenetic relationships, and their potential to hybridize all act to blur taxonomic boundaries. Here we implement a multi-disciplinary approach to evaluate the nature of two deeply-diverged mitochondrial lineages previously documented in Italian tree frogs (Hyla intermedia s. l.), distributed north and south of the Northern Apennine Mountains. Based on evidence from mitochondrial phylogenetics, nuclear phylogenomics, hybrid zone population genomics, niche modeling analyses, and biometric assessments, we propose that these lineages be considered distinct, cryptic species. Both mitochondrial and nuclear data affirm that they belong to two monophyletic clades of Pliocene divergence (~3.5 My), only admixing over a relatively narrow contact zone restricted to the southeast of the Po Plain (50–100 km). These characteristics are comparable to similarly-studied parapatric amphibians bearing a specific status. Inferred from their current geographic distribution, the two Italian tree frogs feature distinct ecological niches (<15% of niche overlap), raising questions regarding potential adaptive components contributing to their incipient speciation. However, we found no diagnostic morphological and bioacoustic differences between them. This system illustrates the speciation continuum of Western-Palearctic tree frogs and identifies additional cryptic lineages of similar divergence to be treated as separate species (H. cf. meridionalis). We recommend combined approaches using genomic data as applied here for the future taxonomic assessment of cryptic diversity in alloparapatric radiations of terrestrial vertebrates, especially in controversial taxa. Finally, we formally described the northern Italian tree frogs as a new species, Hyla perrini sp. nov.urn:lsid:zoobank.org:pub:C446334C-3D94-4820-A851-99BD95084F6D |
| first_indexed | 2024-04-12T19:34:31Z |
| format | Article |
| id | doaj.art-a8239f2b2f774520b14e83932e32a319 |
| institution | Directory Open Access Journal |
| issn | 2296-701X |
| language | English |
| last_indexed | 2024-04-12T19:34:31Z |
| publishDate | 2018-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Ecology and Evolution |
| spelling | doaj.art-a8239f2b2f774520b14e83932e32a3192022-12-22T03:19:15ZengFrontiers Media S.A.Frontiers in Ecology and Evolution2296-701X2018-10-01610.3389/fevo.2018.00144407750Genomic Evidence for Cryptic Speciation in Tree Frogs From the Apennine Peninsula, With Description of Hyla perrini sp. novChristophe Dufresnes0Christophe Dufresnes1Christophe Dufresnes2Glib Mazepa3Glib Mazepa4Nicolas Rodrigues5Alan Brelsford6Alan Brelsford7Spartak N. Litvinchuk8Roberto Sermier9Guillaume Lavanchy10Caroline Betto-Colliard11Olivier Blaser12Amaël Borzée13Elisa Cavoto14Guillaume Fabre15Karim Ghali16Christine Grossen17Agnes Horn18Julien Leuenberger19Barret C. Phillips20Paul A. Saunders21Romain Savary22Tiziano Maddalena23Matthias Stöck24Sylvain Dubey25Sylvain Dubey26Daniele Canestrelli27Daniel L. Jeffries28Department of Ecology and Evolution, University of Lausanne, Lausanne, SwitzerlandDepartment of Animal and Plant Sciences, University of Sheffield, Sheffield, United KingdomHintermann & WeberSA, Montreux, SwitzerlandDepartment of Ecology and Evolution, University of Lausanne, Lausanne, SwitzerlandDepartment of Ecology and Genetics, Evolutionary Biology, Uppsala, SwedenDepartment of Ecology and Evolution, University of Lausanne, Lausanne, SwitzerlandDepartment of Ecology and Evolution, University of Lausanne, Lausanne, SwitzerlandDepartment of Biology, University of California, Riverside, Riverside, CA, United StatesInstitute of Cytology, Russian Academy of Sciences, Saint Petersburg, RussiaDepartment of Ecology and Evolution, University of Lausanne, Lausanne, SwitzerlandDepartment of Ecology and Evolution, University of Lausanne, Lausanne, SwitzerlandDepartment of Ecology and Evolution, University of Lausanne, Lausanne, SwitzerlandDepartment of Ecology and Evolution, University of Lausanne, Lausanne, SwitzerlandDivision of EcoScience and Department of Life Sciences, Ewha Womans University, Seoul, South KoreaDepartment of Ecology and Evolution, University of Lausanne, Lausanne, SwitzerlandDepartment of Ecology and Evolution, University of Lausanne, Lausanne, SwitzerlandDepartment of Ecology and Evolution, University of Lausanne, Lausanne, SwitzerlandDepartment of Ecology and Evolution, University of Lausanne, Lausanne, SwitzerlandDepartment of Ecology and Evolution, University of Lausanne, Lausanne, SwitzerlandDepartment of Ecology and Evolution, University of Lausanne, Lausanne, SwitzerlandDepartment of Ecology and Evolution, University of Lausanne, Lausanne, SwitzerlandDepartment of Ecology and Evolution, University of Lausanne, Lausanne, SwitzerlandDepartment of Ecology and Evolution, University of Lausanne, Lausanne, SwitzerlandMaddalena & Associati sagl, Gordevio, SwitzerlandLeibniz-Institute of Freshwater Ecology and Inland Fisheries (IGB), Berlin, GermanyDepartment of Ecology and Evolution, University of Lausanne, Lausanne, SwitzerlandHintermann & WeberSA, Montreux, Switzerland0Department of Ecological and Biological Sciences, Tuscia University, Viterbo, ItalyDepartment of Ecology and Evolution, University of Lausanne, Lausanne, SwitzerlandDespite increasing appreciation of the speciation continuum, delimiting and describing new species is a major yet necessary challenge of modern phylogeography to help optimize conservation efforts. In amphibians, the lack of phenotypic differences between closely-related taxa, their complex, sometimes unresolved phylogenetic relationships, and their potential to hybridize all act to blur taxonomic boundaries. Here we implement a multi-disciplinary approach to evaluate the nature of two deeply-diverged mitochondrial lineages previously documented in Italian tree frogs (Hyla intermedia s. l.), distributed north and south of the Northern Apennine Mountains. Based on evidence from mitochondrial phylogenetics, nuclear phylogenomics, hybrid zone population genomics, niche modeling analyses, and biometric assessments, we propose that these lineages be considered distinct, cryptic species. Both mitochondrial and nuclear data affirm that they belong to two monophyletic clades of Pliocene divergence (~3.5 My), only admixing over a relatively narrow contact zone restricted to the southeast of the Po Plain (50–100 km). These characteristics are comparable to similarly-studied parapatric amphibians bearing a specific status. Inferred from their current geographic distribution, the two Italian tree frogs feature distinct ecological niches (<15% of niche overlap), raising questions regarding potential adaptive components contributing to their incipient speciation. However, we found no diagnostic morphological and bioacoustic differences between them. This system illustrates the speciation continuum of Western-Palearctic tree frogs and identifies additional cryptic lineages of similar divergence to be treated as separate species (H. cf. meridionalis). We recommend combined approaches using genomic data as applied here for the future taxonomic assessment of cryptic diversity in alloparapatric radiations of terrestrial vertebrates, especially in controversial taxa. Finally, we formally described the northern Italian tree frogs as a new species, Hyla perrini sp. nov.urn:lsid:zoobank.org:pub:C446334C-3D94-4820-A851-99BD95084F6Dhttps://www.frontiersin.org/article/10.3389/fevo.2018.00144/fullamphibiansbioacousticshybrid zonephylogenomicsRAD-seqtaxonomy |
| spellingShingle | Christophe Dufresnes Christophe Dufresnes Christophe Dufresnes Glib Mazepa Glib Mazepa Nicolas Rodrigues Alan Brelsford Alan Brelsford Spartak N. Litvinchuk Roberto Sermier Guillaume Lavanchy Caroline Betto-Colliard Olivier Blaser Amaël Borzée Elisa Cavoto Guillaume Fabre Karim Ghali Christine Grossen Agnes Horn Julien Leuenberger Barret C. Phillips Paul A. Saunders Romain Savary Tiziano Maddalena Matthias Stöck Sylvain Dubey Sylvain Dubey Daniele Canestrelli Daniel L. Jeffries Genomic Evidence for Cryptic Speciation in Tree Frogs From the Apennine Peninsula, With Description of Hyla perrini sp. nov Frontiers in Ecology and Evolution amphibians bioacoustics hybrid zone phylogenomics RAD-seq taxonomy |
| title | Genomic Evidence for Cryptic Speciation in Tree Frogs From the Apennine Peninsula, With Description of Hyla perrini sp. nov |
| title_full | Genomic Evidence for Cryptic Speciation in Tree Frogs From the Apennine Peninsula, With Description of Hyla perrini sp. nov |
| title_fullStr | Genomic Evidence for Cryptic Speciation in Tree Frogs From the Apennine Peninsula, With Description of Hyla perrini sp. nov |
| title_full_unstemmed | Genomic Evidence for Cryptic Speciation in Tree Frogs From the Apennine Peninsula, With Description of Hyla perrini sp. nov |
| title_short | Genomic Evidence for Cryptic Speciation in Tree Frogs From the Apennine Peninsula, With Description of Hyla perrini sp. nov |
| title_sort | genomic evidence for cryptic speciation in tree frogs from the apennine peninsula with description of hyla perrini sp nov |
| topic | amphibians bioacoustics hybrid zone phylogenomics RAD-seq taxonomy |
| url | https://www.frontiersin.org/article/10.3389/fevo.2018.00144/full |
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