The Peroxisomal-CoA Synthetase MoPcs60 Is Important for Fatty Acid Metabolism and Infectious Growth of the Rice Blast Fungus
Fatty acid metabolism is important for the maintenance of fatty acid homeostasis. Free fatty acids, which are toxic in excess, are activated by esterification with coenzyme A (CoA) and then subjected to β-oxidization. Fatty acid β-oxidation-related genes play critical roles in the development and vi...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-01-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2021.811041/full |
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| author | Ting Zhang Ya-Nan Li Xue Li Wangliu Gu Emily Kolojane Moeketsi Ruiwen Zhou Xiaobo Zheng Zhengguang Zhang Haifeng Zhang |
| author_facet | Ting Zhang Ya-Nan Li Xue Li Wangliu Gu Emily Kolojane Moeketsi Ruiwen Zhou Xiaobo Zheng Zhengguang Zhang Haifeng Zhang |
| author_sort | Ting Zhang |
| collection | DOAJ |
| description | Fatty acid metabolism is important for the maintenance of fatty acid homeostasis. Free fatty acids, which are toxic in excess, are activated by esterification with coenzyme A (CoA) and then subjected to β-oxidization. Fatty acid β-oxidation-related genes play critical roles in the development and virulence of several phytopathogens. In this study, we identified and characterized a peroxisomal-CoA synthetase in the rice blast fungus Magnaporthe oryzae, MoPCS60, which is a homolog of PCS60 in budding yeast. MoPCS60 was highly expressed during the conidial and early infectious stages and was induced under oleate treatment. Targeted deletion of MoPCS60 resulted in a significant reduction in growth rate when oleate and olive oil were used as the sole carbon sources. Compared with the wild-type strain Guy11, the ΔMopcs60 mutant exhibited fewer peroxisomes, more lipid droplets, and decreased pathogenicity. The distribution of MoPcs60 varied among developmental stages and was mainly localized to peroxisomes in the hyphae, conidia, and appressoria when treated with oleate. Our results suggest that MoPcs60 is a key peroxisomal-CoA synthetase involved in fatty acid β-oxidation and pathogenicity in rice blast fungi. |
| first_indexed | 2024-12-20T10:25:00Z |
| format | Article |
| id | doaj.art-a823b354b5854e63bd056c1c56297ff6 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-20T10:25:00Z |
| publishDate | 2022-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-a823b354b5854e63bd056c1c56297ff62022-12-21T19:43:50ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-01-011210.3389/fpls.2021.811041811041The Peroxisomal-CoA Synthetase MoPcs60 Is Important for Fatty Acid Metabolism and Infectious Growth of the Rice Blast FungusTing ZhangYa-Nan LiXue LiWangliu GuEmily Kolojane MoeketsiRuiwen ZhouXiaobo ZhengZhengguang ZhangHaifeng ZhangFatty acid metabolism is important for the maintenance of fatty acid homeostasis. Free fatty acids, which are toxic in excess, are activated by esterification with coenzyme A (CoA) and then subjected to β-oxidization. Fatty acid β-oxidation-related genes play critical roles in the development and virulence of several phytopathogens. In this study, we identified and characterized a peroxisomal-CoA synthetase in the rice blast fungus Magnaporthe oryzae, MoPCS60, which is a homolog of PCS60 in budding yeast. MoPCS60 was highly expressed during the conidial and early infectious stages and was induced under oleate treatment. Targeted deletion of MoPCS60 resulted in a significant reduction in growth rate when oleate and olive oil were used as the sole carbon sources. Compared with the wild-type strain Guy11, the ΔMopcs60 mutant exhibited fewer peroxisomes, more lipid droplets, and decreased pathogenicity. The distribution of MoPcs60 varied among developmental stages and was mainly localized to peroxisomes in the hyphae, conidia, and appressoria when treated with oleate. Our results suggest that MoPcs60 is a key peroxisomal-CoA synthetase involved in fatty acid β-oxidation and pathogenicity in rice blast fungi.https://www.frontiersin.org/articles/10.3389/fpls.2021.811041/fullMoPcs60peroxisomeβ-oxidationfatty acid metabolismpathogenicity |
| spellingShingle | Ting Zhang Ya-Nan Li Xue Li Wangliu Gu Emily Kolojane Moeketsi Ruiwen Zhou Xiaobo Zheng Zhengguang Zhang Haifeng Zhang The Peroxisomal-CoA Synthetase MoPcs60 Is Important for Fatty Acid Metabolism and Infectious Growth of the Rice Blast Fungus Frontiers in Plant Science MoPcs60 peroxisome β-oxidation fatty acid metabolism pathogenicity |
| title | The Peroxisomal-CoA Synthetase MoPcs60 Is Important for Fatty Acid Metabolism and Infectious Growth of the Rice Blast Fungus |
| title_full | The Peroxisomal-CoA Synthetase MoPcs60 Is Important for Fatty Acid Metabolism and Infectious Growth of the Rice Blast Fungus |
| title_fullStr | The Peroxisomal-CoA Synthetase MoPcs60 Is Important for Fatty Acid Metabolism and Infectious Growth of the Rice Blast Fungus |
| title_full_unstemmed | The Peroxisomal-CoA Synthetase MoPcs60 Is Important for Fatty Acid Metabolism and Infectious Growth of the Rice Blast Fungus |
| title_short | The Peroxisomal-CoA Synthetase MoPcs60 Is Important for Fatty Acid Metabolism and Infectious Growth of the Rice Blast Fungus |
| title_sort | peroxisomal coa synthetase mopcs60 is important for fatty acid metabolism and infectious growth of the rice blast fungus |
| topic | MoPcs60 peroxisome β-oxidation fatty acid metabolism pathogenicity |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2021.811041/full |
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