Effect of Plant Growth Regulators on Protease Activity in Forest Floor of Norway Spruce Stand
Soil proteases are involved in organic matter transformation processes and, thus, influence ecosystem nutrient turnovers. Phytohormones, similarly to proteases, are synthesized and secreted into soil by fungi and microorganisms, and regulate plant rhizosphere activity. The aim of this study was to d...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-05-01
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| Series: | Forests |
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| Online Access: | https://www.mdpi.com/1999-4907/12/6/665 |
| _version_ | 1797532877732184064 |
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| author | Ladislav Holik Jiří Volánek Valerie Vranová |
| author_facet | Ladislav Holik Jiří Volánek Valerie Vranová |
| author_sort | Ladislav Holik |
| collection | DOAJ |
| description | Soil proteases are involved in organic matter transformation processes and, thus, influence ecosystem nutrient turnovers. Phytohormones, similarly to proteases, are synthesized and secreted into soil by fungi and microorganisms, and regulate plant rhizosphere activity. The aim of this study was to determine the effect of auxins, cytokinins, ethephon, and chlorocholine chloride on spruce forest floor protease activity. It was concluded that the presence of auxins stimulated native proteolytic activity, specifically synthetic auxin 2-naphthoxyacetic acid (16% increase at added quantity of 5 μg) and naturally occurring indole-3-acetic acid (18%, 5 μg). On the contrary, cytokinins, ethephon and chlorocholine chloride inhibited native soil protease activity, where ethephon (36% decrease at 50 μg) and chlorocholine chloride (34%, 100 μg) showed the highest inhibitory effects. It was concluded that negative phytohormonal effects on native proteolytic activity may slow down organic matter decomposition rates and hence complicate plant nutrition. The study enhances the understanding of rhizosphere exudate effects on soil microbial activity and soil nitrogen cycle. |
| first_indexed | 2024-03-10T11:06:37Z |
| format | Article |
| id | doaj.art-a8240ac13af046ae88e10c0665f9cad8 |
| institution | Directory Open Access Journal |
| issn | 1999-4907 |
| language | English |
| last_indexed | 2024-03-10T11:06:37Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Forests |
| spelling | doaj.art-a8240ac13af046ae88e10c0665f9cad82023-11-21T21:09:09ZengMDPI AGForests1999-49072021-05-0112666510.3390/f12060665Effect of Plant Growth Regulators on Protease Activity in Forest Floor of Norway Spruce StandLadislav Holik0Jiří Volánek1Valerie Vranová2Department of Geology and Soil Science, Faculty of Forestry and Wood Technology, Mendel University in Brno, Zemědělská 3, 61300 Brno, Czech RepublicDepartment of Geology and Soil Science, Faculty of Forestry and Wood Technology, Mendel University in Brno, Zemědělská 3, 61300 Brno, Czech RepublicDepartment of Geology and Soil Science, Faculty of Forestry and Wood Technology, Mendel University in Brno, Zemědělská 3, 61300 Brno, Czech RepublicSoil proteases are involved in organic matter transformation processes and, thus, influence ecosystem nutrient turnovers. Phytohormones, similarly to proteases, are synthesized and secreted into soil by fungi and microorganisms, and regulate plant rhizosphere activity. The aim of this study was to determine the effect of auxins, cytokinins, ethephon, and chlorocholine chloride on spruce forest floor protease activity. It was concluded that the presence of auxins stimulated native proteolytic activity, specifically synthetic auxin 2-naphthoxyacetic acid (16% increase at added quantity of 5 μg) and naturally occurring indole-3-acetic acid (18%, 5 μg). On the contrary, cytokinins, ethephon and chlorocholine chloride inhibited native soil protease activity, where ethephon (36% decrease at 50 μg) and chlorocholine chloride (34%, 100 μg) showed the highest inhibitory effects. It was concluded that negative phytohormonal effects on native proteolytic activity may slow down organic matter decomposition rates and hence complicate plant nutrition. The study enhances the understanding of rhizosphere exudate effects on soil microbial activity and soil nitrogen cycle.https://www.mdpi.com/1999-4907/12/6/665auxinscytokininssoil proteasespruce forest |
| spellingShingle | Ladislav Holik Jiří Volánek Valerie Vranová Effect of Plant Growth Regulators on Protease Activity in Forest Floor of Norway Spruce Stand Forests auxins cytokinins soil protease spruce forest |
| title | Effect of Plant Growth Regulators on Protease Activity in Forest Floor of Norway Spruce Stand |
| title_full | Effect of Plant Growth Regulators on Protease Activity in Forest Floor of Norway Spruce Stand |
| title_fullStr | Effect of Plant Growth Regulators on Protease Activity in Forest Floor of Norway Spruce Stand |
| title_full_unstemmed | Effect of Plant Growth Regulators on Protease Activity in Forest Floor of Norway Spruce Stand |
| title_short | Effect of Plant Growth Regulators on Protease Activity in Forest Floor of Norway Spruce Stand |
| title_sort | effect of plant growth regulators on protease activity in forest floor of norway spruce stand |
| topic | auxins cytokinins soil protease spruce forest |
| url | https://www.mdpi.com/1999-4907/12/6/665 |
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