Protocol to detect and quantify tumor hypoxia in mice using photoacoustic imaging
Summary: Photoacoustic imaging (PAI) with co-registered ultrasound (US) is a hybrid non-invasive imaging modality that enables visualization and quantification of tumor hypoxia in live animals. Here, using a breast tumor xenograft model as an example, we present a stepwise protocol describing animal...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-06-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166724001588 |
| _version_ | 1797226643548274688 |
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| author | Tao Dai Laurie J. Rich Mukund Seshadri Subhamoy Dasgupta |
| author_facet | Tao Dai Laurie J. Rich Mukund Seshadri Subhamoy Dasgupta |
| author_sort | Tao Dai |
| collection | DOAJ |
| description | Summary: Photoacoustic imaging (PAI) with co-registered ultrasound (US) is a hybrid non-invasive imaging modality that enables visualization and quantification of tumor hypoxia in live animals. Here, using a breast tumor xenograft model as an example, we present a stepwise protocol describing animal preparation, positioning, instrument setup, and US-PAI image acquisition procedures. This protocol also guides through detailed data analysis, explains functional readouts obtained from PAI, and discusses the potential application of the technology to study the hypoxic tumor microenvironment.For complete details on the use and execution of this protocol, please refer to Dai et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-04-24T14:28:10Z |
| format | Article |
| id | doaj.art-a826ddd6a6fd4f84a8fbd5f975c53713 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-04-24T14:28:10Z |
| publishDate | 2024-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-a826ddd6a6fd4f84a8fbd5f975c537132024-04-03T04:27:28ZengElsevierSTAR Protocols2666-16672024-06-0152102993Protocol to detect and quantify tumor hypoxia in mice using photoacoustic imagingTao Dai0Laurie J. Rich1Mukund Seshadri2Subhamoy Dasgupta3Department of Cell Stress Biology, Roswell Park Comprehensive Cancer Center, Buffalo, NY 14263, USAFUJIFILM VisualSonics, Inc, Toronto, ON, Canada; Department of Oral Oncology, Roswell Park Comprehensive Cancer Center, Buffalo, NY 14263, USADepartment of Oral Oncology, Roswell Park Comprehensive Cancer Center, Buffalo, NY 14263, USA; Corresponding authorDepartment of Cell Stress Biology, Roswell Park Comprehensive Cancer Center, Buffalo, NY 14263, USA; Corresponding authorSummary: Photoacoustic imaging (PAI) with co-registered ultrasound (US) is a hybrid non-invasive imaging modality that enables visualization and quantification of tumor hypoxia in live animals. Here, using a breast tumor xenograft model as an example, we present a stepwise protocol describing animal preparation, positioning, instrument setup, and US-PAI image acquisition procedures. This protocol also guides through detailed data analysis, explains functional readouts obtained from PAI, and discusses the potential application of the technology to study the hypoxic tumor microenvironment.For complete details on the use and execution of this protocol, please refer to Dai et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166724001588BiophysicsCell BiologyCancerModel Organisms |
| spellingShingle | Tao Dai Laurie J. Rich Mukund Seshadri Subhamoy Dasgupta Protocol to detect and quantify tumor hypoxia in mice using photoacoustic imaging STAR Protocols Biophysics Cell Biology Cancer Model Organisms |
| title | Protocol to detect and quantify tumor hypoxia in mice using photoacoustic imaging |
| title_full | Protocol to detect and quantify tumor hypoxia in mice using photoacoustic imaging |
| title_fullStr | Protocol to detect and quantify tumor hypoxia in mice using photoacoustic imaging |
| title_full_unstemmed | Protocol to detect and quantify tumor hypoxia in mice using photoacoustic imaging |
| title_short | Protocol to detect and quantify tumor hypoxia in mice using photoacoustic imaging |
| title_sort | protocol to detect and quantify tumor hypoxia in mice using photoacoustic imaging |
| topic | Biophysics Cell Biology Cancer Model Organisms |
| url | http://www.sciencedirect.com/science/article/pii/S2666166724001588 |
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