Detection of positive mecA Staphylococcus aureus isolated from meat and butchers’ shops by using PCR technique in Mosul city
Methicillin-resistant strains of Staphylococcus aureus (MRSA) can harm to public health as they can cause widespread food poisoning and resistance to multiple antibiotics. Therefore, we aimed to investigate the percentage of S. aureus and MRSA among meat and butcher shop tools, utensils, and workers...
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| Format: | Article |
| Language: | Arabic |
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University of Mosul, College of Veterinary Medicine
2023-10-01
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| Series: | Iraqi Journal of Veterinary Sciences |
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| Online Access: | https://www.vetmedmosul.com/article_180562_41103264c4462f5ef356419c5576a6fa.pdf |
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| author | Omar H. Sheet Omar A. Al-Mahmood Shaker M. Othamn Raad A. Al-Sanjary Ayman H. Alsabawi Amir A. Abdulhak |
| author_facet | Omar H. Sheet Omar A. Al-Mahmood Shaker M. Othamn Raad A. Al-Sanjary Ayman H. Alsabawi Amir A. Abdulhak |
| author_sort | Omar H. Sheet |
| collection | DOAJ |
| description | Methicillin-resistant strains of Staphylococcus aureus (MRSA) can harm to public health as they can cause widespread food poisoning and resistance to multiple antibiotics. Therefore, we aimed to investigate the percentage of S. aureus and MRSA among meat and butcher shop tools, utensils, and workers' hands by detecting the presence of the nuc and mecA genes in S. aureus and MRSA isolates, respectively using PCR technique in Mosul city. In this study, we randomly selected 300 samples from meat and various butcher shop surfaces in Mosul city between September 2021 and January 2022. The findings confirmed that S. aureus carried the nuc gene in meat 64% and other samples, ranging from 36% in machines to 82% in knives. Additionally, out of 178 positive S. aureus isolates in this study, 94 samples showed a positive mecA gene for S. aureus. Significant differences were observed among the meat and various utensils of butcher shops. The meat had a higher prevalence of methicillin-resistant S. aureus isolates reach 75% more than the other tools and utensils (machines 38.9%, tables 36.7%, worker hands 38.5%). Further research is necessary to evaluate the existence of enterotoxin genes in Staphylococcus aureus of different meat products. |
| first_indexed | 2024-03-11T20:40:33Z |
| format | Article |
| id | doaj.art-a82812d357b74c3688331bfe4ea333d4 |
| institution | Directory Open Access Journal |
| issn | 1607-3894 2071-1255 |
| language | Arabic |
| last_indexed | 2024-03-11T20:40:33Z |
| publishDate | 2023-10-01 |
| publisher | University of Mosul, College of Veterinary Medicine |
| record_format | Article |
| series | Iraqi Journal of Veterinary Sciences |
| spelling | doaj.art-a82812d357b74c3688331bfe4ea333d42023-10-02T03:07:56ZaraUniversity of Mosul, College of Veterinary MedicineIraqi Journal of Veterinary Sciences1607-38942071-12552023-10-0137486587010.33899/ijvs.2023.136964.2632180562Detection of positive mecA Staphylococcus aureus isolated from meat and butchers’ shops by using PCR technique in Mosul cityOmar H. Sheet0Omar A. Al-Mahmood1Shaker M. Othamn2Raad A. Al-Sanjary3Ayman H. Alsabawi4Amir A. Abdulhak5Department of Veterinary Public Health, College of Veterinary Medicine, University of Mosul, Mosul, IraqDepartment of Veterinary Public Health, College of Veterinary Medicine, University of Mosul, Mosul, IraqVeterinary Quarr in Parviz Khan, Garmian Veterinary Directorate, Ministry of Agriculture, Erbil, IraqDepartment of Veterinary Public Health, College of Veterinary Medicine, University of Mosul, Mosul, IraqDepartment of Veterinary Public Health, College of Veterinary Medicine, University of Mosul, Mosul, IraqDepartment of Foodborne Zoonoses, Institute of Food Quality and Food Safety, University of Veterinary Medicine, Hannover, GermanyMethicillin-resistant strains of Staphylococcus aureus (MRSA) can harm to public health as they can cause widespread food poisoning and resistance to multiple antibiotics. Therefore, we aimed to investigate the percentage of S. aureus and MRSA among meat and butcher shop tools, utensils, and workers' hands by detecting the presence of the nuc and mecA genes in S. aureus and MRSA isolates, respectively using PCR technique in Mosul city. In this study, we randomly selected 300 samples from meat and various butcher shop surfaces in Mosul city between September 2021 and January 2022. The findings confirmed that S. aureus carried the nuc gene in meat 64% and other samples, ranging from 36% in machines to 82% in knives. Additionally, out of 178 positive S. aureus isolates in this study, 94 samples showed a positive mecA gene for S. aureus. Significant differences were observed among the meat and various utensils of butcher shops. The meat had a higher prevalence of methicillin-resistant S. aureus isolates reach 75% more than the other tools and utensils (machines 38.9%, tables 36.7%, worker hands 38.5%). Further research is necessary to evaluate the existence of enterotoxin genes in Staphylococcus aureus of different meat products.https://www.vetmedmosul.com/article_180562_41103264c4462f5ef356419c5576a6fa.pdfvirulence genemrsafood safetyvealiraq |
| spellingShingle | Omar H. Sheet Omar A. Al-Mahmood Shaker M. Othamn Raad A. Al-Sanjary Ayman H. Alsabawi Amir A. Abdulhak Detection of positive mecA Staphylococcus aureus isolated from meat and butchers’ shops by using PCR technique in Mosul city Iraqi Journal of Veterinary Sciences virulence gene mrsa food safety veal iraq |
| title | Detection of positive mecA Staphylococcus aureus isolated from meat and butchers’ shops by using PCR technique in Mosul city |
| title_full | Detection of positive mecA Staphylococcus aureus isolated from meat and butchers’ shops by using PCR technique in Mosul city |
| title_fullStr | Detection of positive mecA Staphylococcus aureus isolated from meat and butchers’ shops by using PCR technique in Mosul city |
| title_full_unstemmed | Detection of positive mecA Staphylococcus aureus isolated from meat and butchers’ shops by using PCR technique in Mosul city |
| title_short | Detection of positive mecA Staphylococcus aureus isolated from meat and butchers’ shops by using PCR technique in Mosul city |
| title_sort | detection of positive meca staphylococcus aureus isolated from meat and butchers shops by using pcr technique in mosul city |
| topic | virulence gene mrsa food safety veal iraq |
| url | https://www.vetmedmosul.com/article_180562_41103264c4462f5ef356419c5576a6fa.pdf |
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