Opportunistic microbiota of breast milk and antimicrobial activity of milk whey at different periods of lactation
Object of study. The evaluation of the interaction between breast milk opportunistic microorganisms abundance and the milk whey antimicrobial activity at different periods of lactation. Materials and methods. 100 samples of breast milk from healthy breastfeeding mothers were inoculated on solid s...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | Russian |
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Central Research Institute for Epidemiology
2023-04-01
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| Series: | Журнал микробиологии, эпидемиологии и иммунобиологии |
| Subjects: | |
| Online Access: | https://microbiol.crie.ru/jour/article/viewFile/1333/848 |
| _version_ | 1797629296968204288 |
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| author | Vera G. Arzumanyan Tatiana I. Kolyganova Nune O. Vartanova Irina M. Ozhovan Elena O. Kravtsova Vitaly V. Zverev |
| author_facet | Vera G. Arzumanyan Tatiana I. Kolyganova Nune O. Vartanova Irina M. Ozhovan Elena O. Kravtsova Vitaly V. Zverev |
| author_sort | Vera G. Arzumanyan |
| collection | DOAJ |
| description | Object of study. The evaluation of the interaction between breast milk opportunistic microorganisms abundance and the milk whey antimicrobial activity at different periods of lactation.
Materials and methods. 100 samples of breast milk from healthy breastfeeding mothers were inoculated on solid selective media, and then pure cultures of microorganisms were identified by MALDI-TOF mass spectrometry. The antimicrobial activity of the whey against a model culture of Candida albicans was evaluated by spectrophotometry.
Results. 270 isolates represented by 36 species of 13 genera of opportunistic bacteria were obtained. None of the 100 samples contained opportunistic fungi. Staphylococci (7 species) and streptococci (11 species) were predominant. The most common were staphylococci S. epidermidis (70.2%) and S. aureus (20.8%), and streptococci S. mitis (27.7%) and S. oralis (21.8%). The total contamination (median) of opportunistic bacteria in the colostrum was 79 103 CFU/ml, transitional milk 4 103 CFU/ml, mature milk 5 102 CFU/ml. The antimicrobial activity of colostrum whey was 87.489.4%; transitional milk 88.2%; mature milk 63.481.9%. The total contamination had a high inverse correlation with the lactation period (r = 0.806) and a high positive correlation with the antimicrobial activity of whey (r = 0.699). Meanwhile, a significant decrease in contamination was noted after 1 month from the beginning of lactation, while a significant decrease in antimicrobial activity was observed after 8 months.
Conclusions. The decrease of the breast milk contamination by opportunistic bacteria during the lactation period was found to be primary compared to the decrease of the whey antimicrobial activity. Thus, changes in the whey antimicrobial protection factors occur in response to changes of the opportunistic microbiota abundance. |
| first_indexed | 2024-03-11T10:51:14Z |
| format | Article |
| id | doaj.art-a829e38718d54c32b7dc226c9ebe15ce |
| institution | Directory Open Access Journal |
| issn | 0372-9311 2686-7613 |
| language | Russian |
| last_indexed | 2024-03-11T10:51:14Z |
| publishDate | 2023-04-01 |
| publisher | Central Research Institute for Epidemiology |
| record_format | Article |
| series | Журнал микробиологии, эпидемиологии и иммунобиологии |
| spelling | doaj.art-a829e38718d54c32b7dc226c9ebe15ce2023-11-13T17:19:52ZrusCentral Research Institute for EpidemiologyЖурнал микробиологии, эпидемиологии и иммунобиологии0372-93112686-76132023-04-011001748310.36233/0372-9311-352861Opportunistic microbiota of breast milk and antimicrobial activity of milk whey at different periods of lactationVera G. Arzumanyan0https://orcid.org/0000-0001-9769-1634Tatiana I. Kolyganova1https://orcid.org/0000-0002-9065-9786Nune O. Vartanova2https://orcid.org/0000-0002-6372-9910Irina M. Ozhovan3https://orcid.org/0000-0002-8129-0817Elena O. Kravtsova4https://orcid.org/0000-0002-9100-0422Vitaly V. Zverev5https://orcid.org/0000-0001-5808-2246I. Mechnikov Research Institute for Vaccines and SeraI. Mechnikov Research Institute for Vaccines and SeraI. Mechnikov Research Institute for Vaccines and SeraI. Mechnikov Research Institute for Vaccines and SeraFirst Moscow State Medical University (Sechenov University)I. Mechnikov Research Institute for Vaccines and SeraObject of study. The evaluation of the interaction between breast milk opportunistic microorganisms abundance and the milk whey antimicrobial activity at different periods of lactation. Materials and methods. 100 samples of breast milk from healthy breastfeeding mothers were inoculated on solid selective media, and then pure cultures of microorganisms were identified by MALDI-TOF mass spectrometry. The antimicrobial activity of the whey against a model culture of Candida albicans was evaluated by spectrophotometry. Results. 270 isolates represented by 36 species of 13 genera of opportunistic bacteria were obtained. None of the 100 samples contained opportunistic fungi. Staphylococci (7 species) and streptococci (11 species) were predominant. The most common were staphylococci S. epidermidis (70.2%) and S. aureus (20.8%), and streptococci S. mitis (27.7%) and S. oralis (21.8%). The total contamination (median) of opportunistic bacteria in the colostrum was 79 103 CFU/ml, transitional milk 4 103 CFU/ml, mature milk 5 102 CFU/ml. The antimicrobial activity of colostrum whey was 87.489.4%; transitional milk 88.2%; mature milk 63.481.9%. The total contamination had a high inverse correlation with the lactation period (r = 0.806) and a high positive correlation with the antimicrobial activity of whey (r = 0.699). Meanwhile, a significant decrease in contamination was noted after 1 month from the beginning of lactation, while a significant decrease in antimicrobial activity was observed after 8 months. Conclusions. The decrease of the breast milk contamination by opportunistic bacteria during the lactation period was found to be primary compared to the decrease of the whey antimicrobial activity. Thus, changes in the whey antimicrobial protection factors occur in response to changes of the opportunistic microbiota abundance.https://microbiol.crie.ru/jour/article/viewFile/1333/848microbiomebreast milklactation periodsantimicrobial activity |
| spellingShingle | Vera G. Arzumanyan Tatiana I. Kolyganova Nune O. Vartanova Irina M. Ozhovan Elena O. Kravtsova Vitaly V. Zverev Opportunistic microbiota of breast milk and antimicrobial activity of milk whey at different periods of lactation Журнал микробиологии, эпидемиологии и иммунобиологии microbiome breast milk lactation periods antimicrobial activity |
| title | Opportunistic microbiota of breast milk and antimicrobial activity of milk whey at different periods of lactation |
| title_full | Opportunistic microbiota of breast milk and antimicrobial activity of milk whey at different periods of lactation |
| title_fullStr | Opportunistic microbiota of breast milk and antimicrobial activity of milk whey at different periods of lactation |
| title_full_unstemmed | Opportunistic microbiota of breast milk and antimicrobial activity of milk whey at different periods of lactation |
| title_short | Opportunistic microbiota of breast milk and antimicrobial activity of milk whey at different periods of lactation |
| title_sort | opportunistic microbiota of breast milk and antimicrobial activity of milk whey at different periods of lactation |
| topic | microbiome breast milk lactation periods antimicrobial activity |
| url | https://microbiol.crie.ru/jour/article/viewFile/1333/848 |
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