Effect of <i>Agrimonia eupatoria</i> L. and <i>Origanum vulgare</i> L. Leaf, Flower, Stem, and Root Extracts on the Survival of <i>Pseudomonas aeruginosa</i>
<i>Pseudomonas aeruginosa</i> is one of the most antibiotic multi-resistant bacteria, causing chronic pulmonary disease and leading to respiratory failure and even mortality. Thus, there has been an ever-increasing search for novel and preferably natural antimicrobial compounds. <i>...
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MDPI AG
2023-01-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/3/1019 |
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| author | Kateřina Bělonožníková Eliška Sladkovská Daniel Kavan Veronika Hýsková Petr Hodek Daniel Šmíd Helena Ryšlavá |
| author_facet | Kateřina Bělonožníková Eliška Sladkovská Daniel Kavan Veronika Hýsková Petr Hodek Daniel Šmíd Helena Ryšlavá |
| author_sort | Kateřina Bělonožníková |
| collection | DOAJ |
| description | <i>Pseudomonas aeruginosa</i> is one of the most antibiotic multi-resistant bacteria, causing chronic pulmonary disease and leading to respiratory failure and even mortality. Thus, there has been an ever-increasing search for novel and preferably natural antimicrobial compounds. <i>Agrimonia eupatoria</i> L. and <i>Origanum vulgare</i> L. shoots are commonly used as teas or alcoholic tinctures for their human health-promoting and antibacterial properties. Here, we explored the antimicrobial effects of all plant parts, i.e., leaf, flower, stem, and root extracts, prepared in water or in 60% ethanol, against <i>P. aeruginosa</i>. The impact of these extracts on bacterial survival was determined using a luminescent strain of <i>P. aeruginosa</i>, which emits light when alive. In addition, the antimicrobial effects were compared with the antioxidant properties and content of phenolic compounds of plant extracts. Ethanolic extracts of <i>O. vulgare</i> roots and flowers showed the highest antimicrobial activity, followed by <i>A. eupatoria</i> roots. In particular, chlorogenic acid, the ethanolic extract of <i>O. vulgare</i> roots contained high levels of protocatechuic acid, hesperidin, shikimic acid, rutin, quercetin, and morin. The synergistic effects of these phenolic compounds and flavonoids may play a key role in the antibacterial activity of teas and tinctures. |
| first_indexed | 2024-03-11T09:33:36Z |
| format | Article |
| id | doaj.art-a82a77ae8f0b43af95aacf8f920c95d7 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-11T09:33:36Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-a82a77ae8f0b43af95aacf8f920c95d72023-11-16T17:26:30ZengMDPI AGMolecules1420-30492023-01-01283101910.3390/molecules28031019Effect of <i>Agrimonia eupatoria</i> L. and <i>Origanum vulgare</i> L. Leaf, Flower, Stem, and Root Extracts on the Survival of <i>Pseudomonas aeruginosa</i>Kateřina Bělonožníková0Eliška Sladkovská1Daniel Kavan2Veronika Hýsková3Petr Hodek4Daniel Šmíd5Helena Ryšlavá6Department of Biochemistry, Faculty of Science, Charles University, Hlavova 2030, 128 43 Prague, Czech RepublicDepartment of Biochemistry, Faculty of Science, Charles University, Hlavova 2030, 128 43 Prague, Czech RepublicDepartment of Biochemistry, Faculty of Science, Charles University, Hlavova 2030, 128 43 Prague, Czech RepublicDepartment of Biochemistry, Faculty of Science, Charles University, Hlavova 2030, 128 43 Prague, Czech RepublicDepartment of Biochemistry, Faculty of Science, Charles University, Hlavova 2030, 128 43 Prague, Czech RepublicDepartment of Biochemistry, Faculty of Science, Charles University, Hlavova 2030, 128 43 Prague, Czech RepublicDepartment of Biochemistry, Faculty of Science, Charles University, Hlavova 2030, 128 43 Prague, Czech Republic<i>Pseudomonas aeruginosa</i> is one of the most antibiotic multi-resistant bacteria, causing chronic pulmonary disease and leading to respiratory failure and even mortality. Thus, there has been an ever-increasing search for novel and preferably natural antimicrobial compounds. <i>Agrimonia eupatoria</i> L. and <i>Origanum vulgare</i> L. shoots are commonly used as teas or alcoholic tinctures for their human health-promoting and antibacterial properties. Here, we explored the antimicrobial effects of all plant parts, i.e., leaf, flower, stem, and root extracts, prepared in water or in 60% ethanol, against <i>P. aeruginosa</i>. The impact of these extracts on bacterial survival was determined using a luminescent strain of <i>P. aeruginosa</i>, which emits light when alive. In addition, the antimicrobial effects were compared with the antioxidant properties and content of phenolic compounds of plant extracts. Ethanolic extracts of <i>O. vulgare</i> roots and flowers showed the highest antimicrobial activity, followed by <i>A. eupatoria</i> roots. In particular, chlorogenic acid, the ethanolic extract of <i>O. vulgare</i> roots contained high levels of protocatechuic acid, hesperidin, shikimic acid, rutin, quercetin, and morin. The synergistic effects of these phenolic compounds and flavonoids may play a key role in the antibacterial activity of teas and tinctures.https://www.mdpi.com/1420-3049/28/3/1019<i>Agrimonia eupatoria</i> L.<i>Origanum vulgare</i> L.<i>Pseudomonas aeruginosa</i>antimicrobial activityantioxidant capacitybioluminescence |
| spellingShingle | Kateřina Bělonožníková Eliška Sladkovská Daniel Kavan Veronika Hýsková Petr Hodek Daniel Šmíd Helena Ryšlavá Effect of <i>Agrimonia eupatoria</i> L. and <i>Origanum vulgare</i> L. Leaf, Flower, Stem, and Root Extracts on the Survival of <i>Pseudomonas aeruginosa</i> Molecules <i>Agrimonia eupatoria</i> L. <i>Origanum vulgare</i> L. <i>Pseudomonas aeruginosa</i> antimicrobial activity antioxidant capacity bioluminescence |
| title | Effect of <i>Agrimonia eupatoria</i> L. and <i>Origanum vulgare</i> L. Leaf, Flower, Stem, and Root Extracts on the Survival of <i>Pseudomonas aeruginosa</i> |
| title_full | Effect of <i>Agrimonia eupatoria</i> L. and <i>Origanum vulgare</i> L. Leaf, Flower, Stem, and Root Extracts on the Survival of <i>Pseudomonas aeruginosa</i> |
| title_fullStr | Effect of <i>Agrimonia eupatoria</i> L. and <i>Origanum vulgare</i> L. Leaf, Flower, Stem, and Root Extracts on the Survival of <i>Pseudomonas aeruginosa</i> |
| title_full_unstemmed | Effect of <i>Agrimonia eupatoria</i> L. and <i>Origanum vulgare</i> L. Leaf, Flower, Stem, and Root Extracts on the Survival of <i>Pseudomonas aeruginosa</i> |
| title_short | Effect of <i>Agrimonia eupatoria</i> L. and <i>Origanum vulgare</i> L. Leaf, Flower, Stem, and Root Extracts on the Survival of <i>Pseudomonas aeruginosa</i> |
| title_sort | effect of i agrimonia eupatoria i l and i origanum vulgare i l leaf flower stem and root extracts on the survival of i pseudomonas aeruginosa i |
| topic | <i>Agrimonia eupatoria</i> L. <i>Origanum vulgare</i> L. <i>Pseudomonas aeruginosa</i> antimicrobial activity antioxidant capacity bioluminescence |
| url | https://www.mdpi.com/1420-3049/28/3/1019 |
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