Image Contrast Enhancement by Using LED Annular Oblique Illumination in Bright-Field Microscopy

Image Contrast Enhancement by Using LED Annular Oblique Illumination in Bright-Field Microscopy

Köhler illumination is the most used method for evenly illuminating a sample in bright-field microscopy. However, Köhler illumination cannot provide optimum contrast and high resolution simultaneously. The image contrast for Köhler illumination can be enhanced at the expense of lateral resolution. I...

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Bibliographic Details
Main Authors: Xiao Ma, Pan Qi, Zhifeng Wu, Ying Yang, Chengsen Li, Jingang Zhong
Format: Article
Language:English
Published: MDPI AG 2023-04-01
Series:Photonics
Subjects:
bright-field microscopy
contrast enhancement
annular oblique illumination
deconvolution
Online Access:https://www.mdpi.com/2304-6732/10/4/404

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