The Combined Effect of Heat and Osmotic Stress on Suberization of <i>Arabidopsis</i> Roots
The simultaneous occurrence of heat stress and drought is becoming more regular as a consequence of climate change, causing extensive agricultural losses. The application of either heat or osmotic stress increase cell-wall suberization in different tissues, which may play a role in improving plant r...
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MDPI AG
2022-07-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/11/15/2341 |
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| author | Ana Rita Leal Joana Belo Tom Beeckman Pedro M. Barros M. Margarida Oliveira |
| author_facet | Ana Rita Leal Joana Belo Tom Beeckman Pedro M. Barros M. Margarida Oliveira |
| author_sort | Ana Rita Leal |
| collection | DOAJ |
| description | The simultaneous occurrence of heat stress and drought is becoming more regular as a consequence of climate change, causing extensive agricultural losses. The application of either heat or osmotic stress increase cell-wall suberization in different tissues, which may play a role in improving plant resilience. In this work, we studied how the suberization process is affected by the combination of drought and heat stress by following the expression of suberin biosynthesis genes, cell-wall suberization and the chemical composition in <i>Arabidopsis</i> roots. The <i>Arabidopsis</i> plants used in this study were at the onset of secondary root development. At this point, one can observe a developmental gradient in the main root, with primary development closer to the root tip and secondary development, confirmed by the suberized phellem, closer to the shoot. Remarkably, we found a differential response depending on the root zone. The combination of drought and heat stress increased cell wall suberization in main root segments undergoing secondary development and in lateral roots (LRs), while the main root zone, at primary development stage, was not particularly affected. We also found differences in the overall chemical composition of the cell walls in both root zones in response to combined stress. The data gathered showed that, under combined drought and heat stress, <i>Arabidopsis</i> roots undergo differential cell wall remodeling depending on developmental stage, with modifications in the biosynthesis and/or assembly of major cell wall components. |
| first_indexed | 2024-03-09T10:09:44Z |
| format | Article |
| id | doaj.art-a8323b86563743e0be14c8099be324d7 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-09T10:09:44Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-a8323b86563743e0be14c8099be324d72023-12-01T22:52:47ZengMDPI AGCells2073-44092022-07-011115234110.3390/cells11152341The Combined Effect of Heat and Osmotic Stress on Suberization of <i>Arabidopsis</i> RootsAna Rita Leal0Joana Belo1Tom Beeckman2Pedro M. Barros3M. Margarida Oliveira4Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa (ITQB NOVA), GPlantS, Av. da República, 2780-157 Oeiras, PortugalInstituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa (ITQB NOVA), GPlantS, Av. da República, 2780-157 Oeiras, PortugalDepartment of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 71, 9052 Ghent, BelgiumInstituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa (ITQB NOVA), GPlantS, Av. da República, 2780-157 Oeiras, PortugalInstituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa (ITQB NOVA), GPlantS, Av. da República, 2780-157 Oeiras, PortugalThe simultaneous occurrence of heat stress and drought is becoming more regular as a consequence of climate change, causing extensive agricultural losses. The application of either heat or osmotic stress increase cell-wall suberization in different tissues, which may play a role in improving plant resilience. In this work, we studied how the suberization process is affected by the combination of drought and heat stress by following the expression of suberin biosynthesis genes, cell-wall suberization and the chemical composition in <i>Arabidopsis</i> roots. The <i>Arabidopsis</i> plants used in this study were at the onset of secondary root development. At this point, one can observe a developmental gradient in the main root, with primary development closer to the root tip and secondary development, confirmed by the suberized phellem, closer to the shoot. Remarkably, we found a differential response depending on the root zone. The combination of drought and heat stress increased cell wall suberization in main root segments undergoing secondary development and in lateral roots (LRs), while the main root zone, at primary development stage, was not particularly affected. We also found differences in the overall chemical composition of the cell walls in both root zones in response to combined stress. The data gathered showed that, under combined drought and heat stress, <i>Arabidopsis</i> roots undergo differential cell wall remodeling depending on developmental stage, with modifications in the biosynthesis and/or assembly of major cell wall components.https://www.mdpi.com/2073-4409/11/15/2341abiotic stressATR-FTIRplant cell wallsecondary developmentsuberin |
| spellingShingle | Ana Rita Leal Joana Belo Tom Beeckman Pedro M. Barros M. Margarida Oliveira The Combined Effect of Heat and Osmotic Stress on Suberization of <i>Arabidopsis</i> Roots Cells abiotic stress ATR-FTIR plant cell wall secondary development suberin |
| title | The Combined Effect of Heat and Osmotic Stress on Suberization of <i>Arabidopsis</i> Roots |
| title_full | The Combined Effect of Heat and Osmotic Stress on Suberization of <i>Arabidopsis</i> Roots |
| title_fullStr | The Combined Effect of Heat and Osmotic Stress on Suberization of <i>Arabidopsis</i> Roots |
| title_full_unstemmed | The Combined Effect of Heat and Osmotic Stress on Suberization of <i>Arabidopsis</i> Roots |
| title_short | The Combined Effect of Heat and Osmotic Stress on Suberization of <i>Arabidopsis</i> Roots |
| title_sort | combined effect of heat and osmotic stress on suberization of i arabidopsis i roots |
| topic | abiotic stress ATR-FTIR plant cell wall secondary development suberin |
| url | https://www.mdpi.com/2073-4409/11/15/2341 |
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