LIM homeobox protein 5 (Lhx5) is essential for mamillary body development
The mamillary body (MM) is a group of hypothalamic nuclei related to memory and spatial navigation that interconnects hippocampal, thalamic, and tegmental regions. Here we demonstrate that Lhx5, a LIM-HD domain transcription factor expressed early in the developing posterior hypothalamus, is require...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2015-10-01
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| Series: | Frontiers in Neuroanatomy |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fnana.2015.00136/full |
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| author | Amaya eMiquelajauregui Teresa eSandoval-Schaefer Miriam eMartinez-Armenta Leonor ePérez-Martínez Alfonso eCárabez Yangu eZhao Michael eHeide Gonzalo eAlvarez-Bolado Alfredo eVarela-Echavarria |
| author_facet | Amaya eMiquelajauregui Teresa eSandoval-Schaefer Miriam eMartinez-Armenta Leonor ePérez-Martínez Alfonso eCárabez Yangu eZhao Michael eHeide Gonzalo eAlvarez-Bolado Alfredo eVarela-Echavarria |
| author_sort | Amaya eMiquelajauregui |
| collection | DOAJ |
| description | The mamillary body (MM) is a group of hypothalamic nuclei related to memory and spatial navigation that interconnects hippocampal, thalamic, and tegmental regions. Here we demonstrate that Lhx5, a LIM-HD domain transcription factor expressed early in the developing posterior hypothalamus, is required for the generation of the MM and its derived tracts. The MM markers Foxb1, Sim2 and Lhx1 are absent in Lhx5 knock-out mice from early embryonic stages, suggesting abnormal specification of this region. This was supported by the absence of Nkx2.1 and expansion of Shh in the prospective mamillary area. Interestingly, we also found an ectopic domain expressing Lhx2 and Lhx9 along the anterio-posterior hypothalamic axis. Our results suggest that Lhx5 controls early aspects of hypothalamic development by regulating gene expression and cellular specification in the prospective MM. |
| first_indexed | 2024-04-12T23:26:27Z |
| format | Article |
| id | doaj.art-a8324365070b478e8c7a7b15df3bc3f2 |
| institution | Directory Open Access Journal |
| issn | 1662-5129 |
| language | English |
| last_indexed | 2024-04-12T23:26:27Z |
| publishDate | 2015-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Neuroanatomy |
| spelling | doaj.art-a8324365070b478e8c7a7b15df3bc3f22022-12-22T03:12:24ZengFrontiers Media S.A.Frontiers in Neuroanatomy1662-51292015-10-01910.3389/fnana.2015.00136163199LIM homeobox protein 5 (Lhx5) is essential for mamillary body developmentAmaya eMiquelajauregui0Teresa eSandoval-Schaefer1Miriam eMartinez-Armenta2Leonor ePérez-Martínez3Alfonso eCárabez4Yangu eZhao5Michael eHeide6Gonzalo eAlvarez-Bolado7Alfredo eVarela-Echavarria8Instituto de Neurobiologia, Universidad Nacional Autonoma de Mexico (UNAM)Instituto de Neurobiologia, Universidad Nacional Autonoma de Mexico (UNAM)Instituto de Biotecnología, Universidad Nacional Autónoma de México (UNAM)Instituto de Biotecnología, Universidad Nacional Autónoma de México (UNAM)Instituto de Neurobiologia, Universidad Nacional Autonoma de Mexico (UNAM)Eunice Kennedy Shriver National Institute of Child Health and Human Development, NIHInstitute of Anatomy and Cell Biology, University of HeidelbergInstitute of Anatomy and Cell Biology, University of HeidelbergInstituto de Neurobiologia, Universidad Nacional Autonoma de Mexico (UNAM)The mamillary body (MM) is a group of hypothalamic nuclei related to memory and spatial navigation that interconnects hippocampal, thalamic, and tegmental regions. Here we demonstrate that Lhx5, a LIM-HD domain transcription factor expressed early in the developing posterior hypothalamus, is required for the generation of the MM and its derived tracts. The MM markers Foxb1, Sim2 and Lhx1 are absent in Lhx5 knock-out mice from early embryonic stages, suggesting abnormal specification of this region. This was supported by the absence of Nkx2.1 and expansion of Shh in the prospective mamillary area. Interestingly, we also found an ectopic domain expressing Lhx2 and Lhx9 along the anterio-posterior hypothalamic axis. Our results suggest that Lhx5 controls early aspects of hypothalamic development by regulating gene expression and cellular specification in the prospective MM.http://journal.frontiersin.org/Journal/10.3389/fnana.2015.00136/fullDiencephalonEmbryonic DevelopmentHypothalamusMousetranscription factor |
| spellingShingle | Amaya eMiquelajauregui Teresa eSandoval-Schaefer Miriam eMartinez-Armenta Leonor ePérez-Martínez Alfonso eCárabez Yangu eZhao Michael eHeide Gonzalo eAlvarez-Bolado Alfredo eVarela-Echavarria LIM homeobox protein 5 (Lhx5) is essential for mamillary body development Frontiers in Neuroanatomy Diencephalon Embryonic Development Hypothalamus Mouse transcription factor |
| title | LIM homeobox protein 5 (Lhx5) is essential for mamillary body development |
| title_full | LIM homeobox protein 5 (Lhx5) is essential for mamillary body development |
| title_fullStr | LIM homeobox protein 5 (Lhx5) is essential for mamillary body development |
| title_full_unstemmed | LIM homeobox protein 5 (Lhx5) is essential for mamillary body development |
| title_short | LIM homeobox protein 5 (Lhx5) is essential for mamillary body development |
| title_sort | lim homeobox protein 5 lhx5 is essential for mamillary body development |
| topic | Diencephalon Embryonic Development Hypothalamus Mouse transcription factor |
| url | http://journal.frontiersin.org/Journal/10.3389/fnana.2015.00136/full |
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