Morphogenetic identification of a new record Deudorix livia (Lepidoptera: Lycaenidae) in Assiut Governorate of Northern Upper Egypt
Abstract Deudorix livia (Klug, 1834) (Lepidoptera: Lycaenidae) is one of the most serious lepidopteran insect pests attacking pomegranate fruit around the world, including Egypt (Assiut Governorate, Upper Egypt). To create an effective program (IPM) to control such harmful pests, accurate identifica...
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| Format: | Article |
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Nature Portfolio
2023-11-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-46231-8 |
| _version_ | 1797577061287591936 |
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| author | Farouk A. Abdel-Galil Sara E. Mousa Gaber H. Abou-Elhagag Ahmed M. M. Ahmed Ammar Al-Farga Mohammad Allam Mervat A. B. Mahmoud |
| author_facet | Farouk A. Abdel-Galil Sara E. Mousa Gaber H. Abou-Elhagag Ahmed M. M. Ahmed Ammar Al-Farga Mohammad Allam Mervat A. B. Mahmoud |
| author_sort | Farouk A. Abdel-Galil |
| collection | DOAJ |
| description | Abstract Deudorix livia (Klug, 1834) (Lepidoptera: Lycaenidae) is one of the most serious lepidopteran insect pests attacking pomegranate fruit around the world, including Egypt (Assiut Governorate, Upper Egypt). To create an effective program (IPM) to control such harmful pests, accurate identification of the pest morphology and genetic structure is essential. Studies on the morphogenetics of this pest are scarce. So, the goal of this research is to identify it both morphologically and genetically. Pomegranate butterfly immature stages were collected from infested pomegranate fruits and reared in the laboratory until the adult's emergence. By using light and scanning electron microscopy, some morphological structures of males and females were studied. DNA was extracted from the legs of a pomegranate butterfly adult. Also, PCR was conducted by using the mitochondrial CO1 gene for sequencing and phylogenetic tests. The results show that the body scales are a mixture of dark and light gray on the dorsal side and white on the ventral side in both sexes. The average male body length (BL) was 11.674 ± 0.299 mm and was 11.458 ± 1.001 mm for the females. The wing venation is similar in both sexes. For the first time, a partial sequence of the mitochondrial CO1 gene in D. livia was deposited in GenBank (MW463927). |
| first_indexed | 2024-03-10T22:02:35Z |
| format | Article |
| id | doaj.art-a83318eb833e46c9984dba6a31c4b8a8 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-10T22:02:35Z |
| publishDate | 2023-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-a83318eb833e46c9984dba6a31c4b8a82023-11-19T12:54:30ZengNature PortfolioScientific Reports2045-23222023-11-0113111110.1038/s41598-023-46231-8Morphogenetic identification of a new record Deudorix livia (Lepidoptera: Lycaenidae) in Assiut Governorate of Northern Upper EgyptFarouk A. Abdel-Galil0Sara E. Mousa1Gaber H. Abou-Elhagag2Ahmed M. M. Ahmed3Ammar Al-Farga4Mohammad Allam5Mervat A. B. Mahmoud6Plant Protection Department, Faculty of Agriculture, Assiut UniversityPlant Protection Department, Faculty of Agriculture, Assiut UniversityPlant Protection Department, Faculty of Agriculture, Assiut UniversityPlant Protection Department, Faculty of Agriculture, Assiut UniversityDepartment of Biochemistry, College of Sciences, University of JeddahDepartment of Zoology, Faculty of Science, Luxor UniversityZoology Department, Faculty of Science, South Valley UniversityAbstract Deudorix livia (Klug, 1834) (Lepidoptera: Lycaenidae) is one of the most serious lepidopteran insect pests attacking pomegranate fruit around the world, including Egypt (Assiut Governorate, Upper Egypt). To create an effective program (IPM) to control such harmful pests, accurate identification of the pest morphology and genetic structure is essential. Studies on the morphogenetics of this pest are scarce. So, the goal of this research is to identify it both morphologically and genetically. Pomegranate butterfly immature stages were collected from infested pomegranate fruits and reared in the laboratory until the adult's emergence. By using light and scanning electron microscopy, some morphological structures of males and females were studied. DNA was extracted from the legs of a pomegranate butterfly adult. Also, PCR was conducted by using the mitochondrial CO1 gene for sequencing and phylogenetic tests. The results show that the body scales are a mixture of dark and light gray on the dorsal side and white on the ventral side in both sexes. The average male body length (BL) was 11.674 ± 0.299 mm and was 11.458 ± 1.001 mm for the females. The wing venation is similar in both sexes. For the first time, a partial sequence of the mitochondrial CO1 gene in D. livia was deposited in GenBank (MW463927).https://doi.org/10.1038/s41598-023-46231-8 |
| spellingShingle | Farouk A. Abdel-Galil Sara E. Mousa Gaber H. Abou-Elhagag Ahmed M. M. Ahmed Ammar Al-Farga Mohammad Allam Mervat A. B. Mahmoud Morphogenetic identification of a new record Deudorix livia (Lepidoptera: Lycaenidae) in Assiut Governorate of Northern Upper Egypt Scientific Reports |
| title | Morphogenetic identification of a new record Deudorix livia (Lepidoptera: Lycaenidae) in Assiut Governorate of Northern Upper Egypt |
| title_full | Morphogenetic identification of a new record Deudorix livia (Lepidoptera: Lycaenidae) in Assiut Governorate of Northern Upper Egypt |
| title_fullStr | Morphogenetic identification of a new record Deudorix livia (Lepidoptera: Lycaenidae) in Assiut Governorate of Northern Upper Egypt |
| title_full_unstemmed | Morphogenetic identification of a new record Deudorix livia (Lepidoptera: Lycaenidae) in Assiut Governorate of Northern Upper Egypt |
| title_short | Morphogenetic identification of a new record Deudorix livia (Lepidoptera: Lycaenidae) in Assiut Governorate of Northern Upper Egypt |
| title_sort | morphogenetic identification of a new record deudorix livia lepidoptera lycaenidae in assiut governorate of northern upper egypt |
| url | https://doi.org/10.1038/s41598-023-46231-8 |
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