Genetic Profiling and Comparison of Human and Animal Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates from Serbia
The aim of this study was to characterize a collection of methicillin-resistant Staphylococcus aureus (MRSA) isolates of human and animal origin from Serbia. In total, 36 MRSA isolates—30 obtained from humans and six from companion animals—were investigated by PCR for the presenc...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-03-01
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| Series: | Antibiotics |
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| Online Access: | http://www.mdpi.com/2079-6382/8/1/26 |
| _version_ | 1818197879481696256 |
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| author | Jelena Asanin Dusan Misic Ksenija Aksentijevic Zoran Tambur Bojan Rakonjac Ivana Kovacevic Joachim Spergser Igor Loncaric |
| author_facet | Jelena Asanin Dusan Misic Ksenija Aksentijevic Zoran Tambur Bojan Rakonjac Ivana Kovacevic Joachim Spergser Igor Loncaric |
| author_sort | Jelena Asanin |
| collection | DOAJ |
| description | The aim of this study was to characterize a collection of methicillin-resistant Staphylococcus aureus (MRSA) isolates of human and animal origin from Serbia. In total, 36 MRSA isolates—30 obtained from humans and six from companion animals—were investigated by PCR for the presence of antibiotic and biocide resistance determinants and virulence genes (PVL—Panton–Valentine leukocidin, ETs—exfoliative toxins, TSST—toxic shock syndrome toxin, SEs—staphylococcal enterotoxins, and MSCRAMMs—microbial surface components recognizing adhesive matrix molecules and biofilm). Isolates were analyzed by staphylococcal cassette chromosome mec (SCCmec), spa, and dru typing, as well as by multiple locus variable number of tandem repeat analyses (MLVA), multilocus sequence typing (MLST), and subsequently, eBURST. The majority of human MRSA isolates were resistant to gentamicin, erythromycin, clindamycin, and ciprofloxacin. Different antibiotic resistance genes were detected: aac-aphD, ant(6′)-Ia, erm(A), erm(B), erm(C), tet(K), tet(M), fexA, and catpC221. All isolates were susceptible to teicoplanin and linezolid. SCCmec type III was prevalent in human isolates, while SCCmec elements in animals were mostly nontypeable. t037 was the predominant spa type in human and t242 in animal MRSA isolates. The prevalent dru type was dt11c in human and dt10a in animal MRSA isolates. MRSA isolates exhibited 27 different MLVA types. ST239 was predominant in human, while ST5 was prevalent in canine MRSA isolates. PVL was found in two, while tsst-1 was detected in three human isolates. Human-associated clones belonging to ST5, ST45, and ST239 MRSA clones were discovered in companion animals, which suggests anthropozoonotic transmission. |
| first_indexed | 2024-12-12T01:56:59Z |
| format | Article |
| id | doaj.art-a83f099525bb4dd7ace0d24016190426 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-12-12T01:56:59Z |
| publishDate | 2019-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-a83f099525bb4dd7ace0d240161904262022-12-22T00:42:20ZengMDPI AGAntibiotics2079-63822019-03-01812610.3390/antibiotics8010026antibiotics8010026Genetic Profiling and Comparison of Human and Animal Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates from SerbiaJelena Asanin0Dusan Misic1Ksenija Aksentijevic2Zoran Tambur3Bojan Rakonjac4Ivana Kovacevic5Joachim Spergser6Igor Loncaric7Innovation Center of Faculty of Technology and Metallurgy, University of Belgrade, 11000 Beograd, SerbiaFaculty of Veterinary Medicine, University of Belgrade, 11000 Beograd, SerbiaFaculty of Veterinary Medicine, University of Belgrade, 11000 Beograd, SerbiaInstitute of Hygiene Military Medical Academy, Belgrade, Serbia and Faculty of Stomatology in Pancevo, 11000 Beograd, SerbiaInstitute of Microbiology Military Medical Academy, 11000 Beograd, SerbiaInstitute of Hygiene Military Medical Academy, 11000 Beograd, SerbiaInstitute of Microbiology, University of Veterinary Medicine, 1210 Wien, AustriaInstitute of Microbiology, University of Veterinary Medicine, 1210 Wien, AustriaThe aim of this study was to characterize a collection of methicillin-resistant Staphylococcus aureus (MRSA) isolates of human and animal origin from Serbia. In total, 36 MRSA isolates—30 obtained from humans and six from companion animals—were investigated by PCR for the presence of antibiotic and biocide resistance determinants and virulence genes (PVL—Panton–Valentine leukocidin, ETs—exfoliative toxins, TSST—toxic shock syndrome toxin, SEs—staphylococcal enterotoxins, and MSCRAMMs—microbial surface components recognizing adhesive matrix molecules and biofilm). Isolates were analyzed by staphylococcal cassette chromosome mec (SCCmec), spa, and dru typing, as well as by multiple locus variable number of tandem repeat analyses (MLVA), multilocus sequence typing (MLST), and subsequently, eBURST. The majority of human MRSA isolates were resistant to gentamicin, erythromycin, clindamycin, and ciprofloxacin. Different antibiotic resistance genes were detected: aac-aphD, ant(6′)-Ia, erm(A), erm(B), erm(C), tet(K), tet(M), fexA, and catpC221. All isolates were susceptible to teicoplanin and linezolid. SCCmec type III was prevalent in human isolates, while SCCmec elements in animals were mostly nontypeable. t037 was the predominant spa type in human and t242 in animal MRSA isolates. The prevalent dru type was dt11c in human and dt10a in animal MRSA isolates. MRSA isolates exhibited 27 different MLVA types. ST239 was predominant in human, while ST5 was prevalent in canine MRSA isolates. PVL was found in two, while tsst-1 was detected in three human isolates. Human-associated clones belonging to ST5, ST45, and ST239 MRSA clones were discovered in companion animals, which suggests anthropozoonotic transmission.http://www.mdpi.com/2079-6382/8/1/26MRSAhumansanimalsantibiotic resistanceSCCmec typingdru typingspa typingMLVAMLST |
| spellingShingle | Jelena Asanin Dusan Misic Ksenija Aksentijevic Zoran Tambur Bojan Rakonjac Ivana Kovacevic Joachim Spergser Igor Loncaric Genetic Profiling and Comparison of Human and Animal Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates from Serbia Antibiotics MRSA humans animals antibiotic resistance SCCmec typing dru typing spa typing MLVA MLST |
| title | Genetic Profiling and Comparison of Human and Animal Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates from Serbia |
| title_full | Genetic Profiling and Comparison of Human and Animal Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates from Serbia |
| title_fullStr | Genetic Profiling and Comparison of Human and Animal Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates from Serbia |
| title_full_unstemmed | Genetic Profiling and Comparison of Human and Animal Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates from Serbia |
| title_short | Genetic Profiling and Comparison of Human and Animal Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates from Serbia |
| title_sort | genetic profiling and comparison of human and animal methicillin resistant staphylococcus aureus mrsa isolates from serbia |
| topic | MRSA humans animals antibiotic resistance SCCmec typing dru typing spa typing MLVA MLST |
| url | http://www.mdpi.com/2079-6382/8/1/26 |
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