Different Expression Analysis in Fruit Softening and Ethylene Biosynthetic Pathways in Peaches of Different Flesh Textures
The aim of our study was to assess differences in the expression of genes involved in fruit softening and ethylene biosynthetic pathways under different temperature storage conditions. Different peach cultivars of ‘Xiacui’ and ‘Yumyeong’, which are stonyhard, ‘Yinhualu’, which is soft-melting, ‘Huji...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2016-03-01
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| Series: | Horticultural Plant Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468014116300334 |
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| author | Yong Yang Ruijuan Ma Binbin Zhang Zhizhong Song Chunhua Zhang Shaolei Guo Mingliang Yu |
| author_facet | Yong Yang Ruijuan Ma Binbin Zhang Zhizhong Song Chunhua Zhang Shaolei Guo Mingliang Yu |
| author_sort | Yong Yang |
| collection | DOAJ |
| description | The aim of our study was to assess differences in the expression of genes involved in fruit softening and ethylene biosynthetic pathways under different temperature storage conditions. Different peach cultivars of ‘Xiacui’ and ‘Yumyeong’, which are stonyhard, ‘Yinhualu’, which is soft-melting, ‘Hujing Milu’, which is hard-melting, and ‘Baby Gold 6’, which is non-melting at 80% ripening, were collected as test materials. The results showed that only slight ethylene production was detected after harvesting of ‘Yumyeong’ and ‘Xiacui’ under either a room temperature (25 °C) or low temperature (4 °C). The fruit firmness of stonyhard cultivars was retained at a high level under room temperature over time, whereas a low temperature induced ‘Yumyeong’ fruit to soften. Quantitative real-time PCR results indicated that the PpACS1 gene was highly expressed in soft-melting, hard-melting and non-melting cultivars; however, expression was extremely low in stonyhard peaches. PpACS2 or PpACS3, however, was not detected in all five cultivars. Interestingly, cold treatment significantly decreased firmness along with endo-PG expression obviously up-regulated in ‘Yumyeong’, but not in ‘Xiacui’ peaches. In conclusion, this study revealed that fruit softening of peaches with different flesh textures was closely related to ethylene biosynthesis during the storage period, which was controlled via regulating relevant gene expression levels under different storage temperatures. |
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| institution | Directory Open Access Journal |
| issn | 2468-0141 |
| language | English |
| last_indexed | 2024-04-24T08:29:20Z |
| publishDate | 2016-03-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Horticultural Plant Journal |
| spelling | doaj.art-a842404dff284563b5d7c3446e95257c2024-04-16T20:42:07ZengKeAi Communications Co., Ltd.Horticultural Plant Journal2468-01412016-03-0122758110.1016/j.hpj.2016.06.006Different Expression Analysis in Fruit Softening and Ethylene Biosynthetic Pathways in Peaches of Different Flesh TexturesYong Yang0Ruijuan Ma1Binbin Zhang2Zhizhong Song3Chunhua Zhang4Shaolei Guo5Mingliang Yu6Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Institute of Horticulture, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaJiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Institute of Horticulture, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaJiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Institute of Horticulture, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaJiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Institute of Horticulture, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaJiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Institute of Horticulture, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaJiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Institute of Horticulture, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaJiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Institute of Horticulture, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaThe aim of our study was to assess differences in the expression of genes involved in fruit softening and ethylene biosynthetic pathways under different temperature storage conditions. Different peach cultivars of ‘Xiacui’ and ‘Yumyeong’, which are stonyhard, ‘Yinhualu’, which is soft-melting, ‘Hujing Milu’, which is hard-melting, and ‘Baby Gold 6’, which is non-melting at 80% ripening, were collected as test materials. The results showed that only slight ethylene production was detected after harvesting of ‘Yumyeong’ and ‘Xiacui’ under either a room temperature (25 °C) or low temperature (4 °C). The fruit firmness of stonyhard cultivars was retained at a high level under room temperature over time, whereas a low temperature induced ‘Yumyeong’ fruit to soften. Quantitative real-time PCR results indicated that the PpACS1 gene was highly expressed in soft-melting, hard-melting and non-melting cultivars; however, expression was extremely low in stonyhard peaches. PpACS2 or PpACS3, however, was not detected in all five cultivars. Interestingly, cold treatment significantly decreased firmness along with endo-PG expression obviously up-regulated in ‘Yumyeong’, but not in ‘Xiacui’ peaches. In conclusion, this study revealed that fruit softening of peaches with different flesh textures was closely related to ethylene biosynthesis during the storage period, which was controlled via regulating relevant gene expression levels under different storage temperatures.http://www.sciencedirect.com/science/article/pii/S2468014116300334Prunus persicaethylenebiosynthetic pathwaysoftengene expression |
| spellingShingle | Yong Yang Ruijuan Ma Binbin Zhang Zhizhong Song Chunhua Zhang Shaolei Guo Mingliang Yu Different Expression Analysis in Fruit Softening and Ethylene Biosynthetic Pathways in Peaches of Different Flesh Textures Horticultural Plant Journal Prunus persica ethylene biosynthetic pathway soften gene expression |
| title | Different Expression Analysis in Fruit Softening and Ethylene Biosynthetic Pathways in Peaches of Different Flesh Textures |
| title_full | Different Expression Analysis in Fruit Softening and Ethylene Biosynthetic Pathways in Peaches of Different Flesh Textures |
| title_fullStr | Different Expression Analysis in Fruit Softening and Ethylene Biosynthetic Pathways in Peaches of Different Flesh Textures |
| title_full_unstemmed | Different Expression Analysis in Fruit Softening and Ethylene Biosynthetic Pathways in Peaches of Different Flesh Textures |
| title_short | Different Expression Analysis in Fruit Softening and Ethylene Biosynthetic Pathways in Peaches of Different Flesh Textures |
| title_sort | different expression analysis in fruit softening and ethylene biosynthetic pathways in peaches of different flesh textures |
| topic | Prunus persica ethylene biosynthetic pathway soften gene expression |
| url | http://www.sciencedirect.com/science/article/pii/S2468014116300334 |
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