Development of a current controlled stimulation setup for investigating the effect of electrical currents on implant infections caused by biofilms
Introduction: Biofilms are bacterial communities that transform into a state enclosed in an extracellular polymeric substance, which makes them less susceptible to antibiotics. Those bacterial formations often develop on metal implants and cause chronic infections. Due to the severely reduced impact...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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De Gruyter
2023-09-01
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| Series: | Current Directions in Biomedical Engineering |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/cdbme-2023-1028 |
| _version_ | 1797646364707913728 |
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| author | Fenko Maxim Valtin Markus Wiesener Constantin Fanaei Pirlar Rima Trampuz Andrej Schauer Thomas |
| author_facet | Fenko Maxim Valtin Markus Wiesener Constantin Fanaei Pirlar Rima Trampuz Andrej Schauer Thomas |
| author_sort | Fenko Maxim |
| collection | DOAJ |
| description | Introduction: Biofilms are bacterial communities that transform into a state enclosed in an extracellular polymeric substance, which makes them less susceptible to antibiotics. Those bacterial formations often develop on metal implants and cause chronic infections. Due to the severely reduced impact of antibiotics against biofilms, the effect of electric stimulation (on its own and in combination with antimicrobials) needs to be further observed, as available studies indicate a positive effect. Methods: Therefore, this work examined the development of a six-channel current-controlled stimulation setup, which enables further in vitro research on the effects of electric stimulation on biofilms. The setup controls the desired stimulation current through the load to counteract electrochemical processes, which constantly change its resistive and capacitive properties. Results: Each channel of the stimulator is able to operate within an amplitude range of 50 μA to 1 mA, a frequency range of 0 Hz to 1 kHz, and a pulse width range of 50 μs to 1 ms. The current control provides a sufficient rise time of 3.3 μs for three different stimulation modes: constant direct current (DC), pulsed DC, and biphasic-pulsed alternating current (AC). Furthermore, a graphical user interface enables the user to regulate and observe the stimulation on a computer to which the stimulator device is connected. Conclusion: The achieved variety of stimulation parameters in one device makes it possible to analyze the effect of different stimulation paradigms on biofilms and therefore enables more in vitro research which is inevitable to develop a sufficient treatment for patients with biofilm-infected implants. |
| first_indexed | 2024-03-11T15:01:03Z |
| format | Article |
| id | doaj.art-a84267f5b5d44d7f91dba3b389f94c44 |
| institution | Directory Open Access Journal |
| issn | 2364-5504 |
| language | English |
| last_indexed | 2024-03-11T15:01:03Z |
| publishDate | 2023-09-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Current Directions in Biomedical Engineering |
| spelling | doaj.art-a84267f5b5d44d7f91dba3b389f94c442023-10-30T07:58:11ZengDe GruyterCurrent Directions in Biomedical Engineering2364-55042023-09-019110911210.1515/cdbme-2023-1028Development of a current controlled stimulation setup for investigating the effect of electrical currents on implant infections caused by biofilmsFenko Maxim0Valtin Markus1Wiesener Constantin2Fanaei Pirlar Rima3Trampuz Andrej4Schauer Thomas5Technische Universität Berlin, Control Systems Group, Berlin, GermanyTechnische Universität Berlin, Control Systems Group, Berlin, GermanySensorStim Neurotechnology GmbH, Berlin, GermanyCharité - Universitätsmedizin Berlin, Berlin, GermanyCharité – Universitätsmedizin Berlin, Berlin, GermanyTechnische Universität Berlin, Control Systems Group, Berlin, GermanyIntroduction: Biofilms are bacterial communities that transform into a state enclosed in an extracellular polymeric substance, which makes them less susceptible to antibiotics. Those bacterial formations often develop on metal implants and cause chronic infections. Due to the severely reduced impact of antibiotics against biofilms, the effect of electric stimulation (on its own and in combination with antimicrobials) needs to be further observed, as available studies indicate a positive effect. Methods: Therefore, this work examined the development of a six-channel current-controlled stimulation setup, which enables further in vitro research on the effects of electric stimulation on biofilms. The setup controls the desired stimulation current through the load to counteract electrochemical processes, which constantly change its resistive and capacitive properties. Results: Each channel of the stimulator is able to operate within an amplitude range of 50 μA to 1 mA, a frequency range of 0 Hz to 1 kHz, and a pulse width range of 50 μs to 1 ms. The current control provides a sufficient rise time of 3.3 μs for three different stimulation modes: constant direct current (DC), pulsed DC, and biphasic-pulsed alternating current (AC). Furthermore, a graphical user interface enables the user to regulate and observe the stimulation on a computer to which the stimulator device is connected. Conclusion: The achieved variety of stimulation parameters in one device makes it possible to analyze the effect of different stimulation paradigms on biofilms and therefore enables more in vitro research which is inevitable to develop a sufficient treatment for patients with biofilm-infected implants.https://doi.org/10.1515/cdbme-2023-1028electric stimulation (es)biofilmimplants |
| spellingShingle | Fenko Maxim Valtin Markus Wiesener Constantin Fanaei Pirlar Rima Trampuz Andrej Schauer Thomas Development of a current controlled stimulation setup for investigating the effect of electrical currents on implant infections caused by biofilms Current Directions in Biomedical Engineering electric stimulation (es) biofilm implants |
| title | Development of a current controlled stimulation setup for investigating the effect of electrical currents on implant infections caused by biofilms |
| title_full | Development of a current controlled stimulation setup for investigating the effect of electrical currents on implant infections caused by biofilms |
| title_fullStr | Development of a current controlled stimulation setup for investigating the effect of electrical currents on implant infections caused by biofilms |
| title_full_unstemmed | Development of a current controlled stimulation setup for investigating the effect of electrical currents on implant infections caused by biofilms |
| title_short | Development of a current controlled stimulation setup for investigating the effect of electrical currents on implant infections caused by biofilms |
| title_sort | development of a current controlled stimulation setup for investigating the effect of electrical currents on implant infections caused by biofilms |
| topic | electric stimulation (es) biofilm implants |
| url | https://doi.org/10.1515/cdbme-2023-1028 |
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