In Vitro Production of Calcified Bone Matrix onto Wool Keratin Scaffolds via Osteogenic Factors and Electromagnetic Stimulus
Pulsed electromagnetic field (PEMF) has drawn attention as a potential tool to improve the ability of bone biomaterials to integrate into the surrounding tissue. We investigated the effects of PEMF (frequency, 75 Hz; magnetic induction amplitude, 2 mT; pulse duration, 1.3 ms) on human osteoblast-lik...
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2020-07-01
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| Online Access: | https://www.mdpi.com/1996-1944/13/14/3052 |
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| author | Nora Bloise Alessia Patrucco Giovanna Bruni Giulia Montagna Rosalinda Caringella Lorenzo Fassina Claudio Tonin Livia Visai |
| author_facet | Nora Bloise Alessia Patrucco Giovanna Bruni Giulia Montagna Rosalinda Caringella Lorenzo Fassina Claudio Tonin Livia Visai |
| author_sort | Nora Bloise |
| collection | DOAJ |
| description | Pulsed electromagnetic field (PEMF) has drawn attention as a potential tool to improve the ability of bone biomaterials to integrate into the surrounding tissue. We investigated the effects of PEMF (frequency, 75 Hz; magnetic induction amplitude, 2 mT; pulse duration, 1.3 ms) on human osteoblast-like cells (SAOS-2) seeded onto wool keratin scaffolds in terms of proliferation, differentiation, and production of the calcified bone extracellular matrix. The wool keratin scaffold offered a 3D porous architecture for cell guesting and nutrient diffusion, suggesting its possible use as a filler to repair bone defects. Here, the combined approach of applying a daily PEMF exposure with additional osteogenic factors stimulated the cells to increase both the deposition of bone-related proteins and calcified matrix onto the wool keratin scaffolds. Also, the presence of SAOS-2 cells, or PEMF, or osteogenic factors did not influence the compression behavior or the resilience of keratin scaffolds in wet conditions. Besides, ageing tests revealed that wool keratin scaffolds were very stable and showed a lower degradation rate compared to commercial collagen sponges. It is for these reasons that this tissue engineering strategy, which improves the osteointegration properties of the wool keratin scaffold, may have a promising application for long term support of bone formation in vivo. |
| first_indexed | 2024-03-10T18:36:48Z |
| format | Article |
| id | doaj.art-a846e248706042de918ec67f0b975d60 |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-10T18:36:48Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Materials |
| spelling | doaj.art-a846e248706042de918ec67f0b975d602023-11-20T06:10:40ZengMDPI AGMaterials1996-19442020-07-011314305210.3390/ma13143052In Vitro Production of Calcified Bone Matrix onto Wool Keratin Scaffolds via Osteogenic Factors and Electromagnetic StimulusNora Bloise0Alessia Patrucco1Giovanna Bruni2Giulia Montagna3Rosalinda Caringella4Lorenzo Fassina5Claudio Tonin6Livia Visai7Department of Molecular Medicine (DMM), Centre for Health Technologies (CHT), UdR INSTM, University of Pavia, Viale Taramelli, 3/B-27100 Pavia, ItalyInstitute of Intelligent Industrial Technologies and Systems for Advanced Manufacturing (STIIMA), Italian National Research Council (CNR), Corso Pella, 16-13900 Biella, ItalyCenter for Colloid and Surface Science (C.S.G.I.), Department of Chemistry, Section of Physical Chemistry, University of Pavia, Viale Taramelli, 16-27100 Pavia, ItalyDepartment of Molecular Medicine (DMM), Centre for Health Technologies (CHT), UdR INSTM, University of Pavia, Viale Taramelli, 3/B-27100 Pavia, ItalyInstitute of Intelligent Industrial Technologies and Systems for Advanced Manufacturing (STIIMA), Italian National Research Council (CNR), Corso Pella, 16-13900 Biella, ItalyDepartment of Electrical, Computer and Biomedical Engineering (DIII), Centre for Health Technologies (CHT), University of Pavia, Via Ferrata, 5-27100 Pavia, ItalyInstitute of Intelligent Industrial Technologies and Systems for Advanced Manufacturing (STIIMA), Italian National Research Council (CNR), Corso Pella, 16-13900 Biella, ItalyDepartment of Molecular Medicine (DMM), Centre for Health Technologies (CHT), UdR INSTM, University of Pavia, Viale Taramelli, 3/B-27100 Pavia, ItalyPulsed electromagnetic field (PEMF) has drawn attention as a potential tool to improve the ability of bone biomaterials to integrate into the surrounding tissue. We investigated the effects of PEMF (frequency, 75 Hz; magnetic induction amplitude, 2 mT; pulse duration, 1.3 ms) on human osteoblast-like cells (SAOS-2) seeded onto wool keratin scaffolds in terms of proliferation, differentiation, and production of the calcified bone extracellular matrix. The wool keratin scaffold offered a 3D porous architecture for cell guesting and nutrient diffusion, suggesting its possible use as a filler to repair bone defects. Here, the combined approach of applying a daily PEMF exposure with additional osteogenic factors stimulated the cells to increase both the deposition of bone-related proteins and calcified matrix onto the wool keratin scaffolds. Also, the presence of SAOS-2 cells, or PEMF, or osteogenic factors did not influence the compression behavior or the resilience of keratin scaffolds in wet conditions. Besides, ageing tests revealed that wool keratin scaffolds were very stable and showed a lower degradation rate compared to commercial collagen sponges. It is for these reasons that this tissue engineering strategy, which improves the osteointegration properties of the wool keratin scaffold, may have a promising application for long term support of bone formation in vivo.https://www.mdpi.com/1996-1944/13/14/3052pulsed electromagnetic fieldosteogenic factorswool keratin scaffoldsbone tissue engineering |
| spellingShingle | Nora Bloise Alessia Patrucco Giovanna Bruni Giulia Montagna Rosalinda Caringella Lorenzo Fassina Claudio Tonin Livia Visai In Vitro Production of Calcified Bone Matrix onto Wool Keratin Scaffolds via Osteogenic Factors and Electromagnetic Stimulus Materials pulsed electromagnetic field osteogenic factors wool keratin scaffolds bone tissue engineering |
| title | In Vitro Production of Calcified Bone Matrix onto Wool Keratin Scaffolds via Osteogenic Factors and Electromagnetic Stimulus |
| title_full | In Vitro Production of Calcified Bone Matrix onto Wool Keratin Scaffolds via Osteogenic Factors and Electromagnetic Stimulus |
| title_fullStr | In Vitro Production of Calcified Bone Matrix onto Wool Keratin Scaffolds via Osteogenic Factors and Electromagnetic Stimulus |
| title_full_unstemmed | In Vitro Production of Calcified Bone Matrix onto Wool Keratin Scaffolds via Osteogenic Factors and Electromagnetic Stimulus |
| title_short | In Vitro Production of Calcified Bone Matrix onto Wool Keratin Scaffolds via Osteogenic Factors and Electromagnetic Stimulus |
| title_sort | in vitro production of calcified bone matrix onto wool keratin scaffolds via osteogenic factors and electromagnetic stimulus |
| topic | pulsed electromagnetic field osteogenic factors wool keratin scaffolds bone tissue engineering |
| url | https://www.mdpi.com/1996-1944/13/14/3052 |
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