Effects of Hyperoxia and Hyperoxic Oscillations on the Proteome of Murine Lung Microvascular Endothelium
Patients presenting with insufficient tissue oxygenation and impaired lung function as in acute respiratory distress syndrome (ARDS) frequently require mechanical ventilation with supplemental oxygen. Despite the lung being used to experiencing the highest partial pressure of oxygen during healthy b...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/11/12/2349 |
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| author | Akos Tiboldi Eva Hunyadi-Gulyas Peter Wohlrab Johannes A. Schmid Klaus Markstaller Klaus Ulrich Klein Verena Tretter |
| author_facet | Akos Tiboldi Eva Hunyadi-Gulyas Peter Wohlrab Johannes A. Schmid Klaus Markstaller Klaus Ulrich Klein Verena Tretter |
| author_sort | Akos Tiboldi |
| collection | DOAJ |
| description | Patients presenting with insufficient tissue oxygenation and impaired lung function as in acute respiratory distress syndrome (ARDS) frequently require mechanical ventilation with supplemental oxygen. Despite the lung being used to experiencing the highest partial pressure of oxygen during healthy breathing, the organ is susceptible to oxygen-induced injury at supraphysiological concentrations. Hyperoxia-induced lung injury (HALI) has been regarded as a second hit to pre-existing lung injury and ventilator-induced lung injury (VILI) attributed to oxidative stress. The injured lung has a tendency to form atelectasis, a cyclic collapse and reopening of alveoli. The affected lung areas experience oxygen conditions that oscillate between hyperoxia and hypoxia rather than remaining in a constant hyperoxic state. Mechanisms of HALI have been investigated in many animal models previously. These studies provided insights into the effects of hyperoxia on the whole organism. However, cell type-specific responses have not been dissected in detail, but are necessary for a complete mechanistic understanding of ongoing pathological processes. In our study, we investigated the effects of constant and intermittent hyperoxia on the lung endothelium from a mouse by an in vitro proteomic approach. We demonstrate that these oxygen conditions have characteristic effects on the pulmonary endothelial proteome that underlie the physiological (patho)mechanisms. |
| first_indexed | 2024-03-09T17:24:15Z |
| format | Article |
| id | doaj.art-a84a9307baba43b5983bc25b2a4dfa76 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-09T17:24:15Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-a84a9307baba43b5983bc25b2a4dfa762023-11-24T12:56:32ZengMDPI AGAntioxidants2076-39212022-11-011112234910.3390/antiox11122349Effects of Hyperoxia and Hyperoxic Oscillations on the Proteome of Murine Lung Microvascular EndotheliumAkos Tiboldi0Eva Hunyadi-Gulyas1Peter Wohlrab2Johannes A. Schmid3Klaus Markstaller4Klaus Ulrich Klein5Verena Tretter6Department of Anesthesia, General Intensive Care and Pain Management, Medical University of Vienna, 1090 Vienna, AustriaLaboratory of Proteomics Research, Biological Research Centre, Eötvös Loránd Research Network (ELKH), Temesvári krt. 62, H-6726 Szeged, HungaryDepartment of Anesthesia, General Intensive Care and Pain Management, Medical University of Vienna, 1090 Vienna, AustriaDepartment of Vascular Biology and Thrombosis Research, Center for Physiology and Pharmacology, Medical University Vienna, Schwarzspanierstraße 17, 1090 Vienna, AustriaDepartment of Anesthesia, General Intensive Care and Pain Management, Medical University of Vienna, 1090 Vienna, AustriaDepartment of Anesthesia, General Intensive Care and Pain Management, Medical University of Vienna, 1090 Vienna, AustriaDepartment of Anesthesia, General Intensive Care and Pain Management, Medical University of Vienna, 1090 Vienna, AustriaPatients presenting with insufficient tissue oxygenation and impaired lung function as in acute respiratory distress syndrome (ARDS) frequently require mechanical ventilation with supplemental oxygen. Despite the lung being used to experiencing the highest partial pressure of oxygen during healthy breathing, the organ is susceptible to oxygen-induced injury at supraphysiological concentrations. Hyperoxia-induced lung injury (HALI) has been regarded as a second hit to pre-existing lung injury and ventilator-induced lung injury (VILI) attributed to oxidative stress. The injured lung has a tendency to form atelectasis, a cyclic collapse and reopening of alveoli. The affected lung areas experience oxygen conditions that oscillate between hyperoxia and hypoxia rather than remaining in a constant hyperoxic state. Mechanisms of HALI have been investigated in many animal models previously. These studies provided insights into the effects of hyperoxia on the whole organism. However, cell type-specific responses have not been dissected in detail, but are necessary for a complete mechanistic understanding of ongoing pathological processes. In our study, we investigated the effects of constant and intermittent hyperoxia on the lung endothelium from a mouse by an in vitro proteomic approach. We demonstrate that these oxygen conditions have characteristic effects on the pulmonary endothelial proteome that underlie the physiological (patho)mechanisms.https://www.mdpi.com/2076-3921/11/12/2349acute respiratory distress syndromehyperoxic acute lung injurylung microvascular endothelial cellsventilator-induced lung injuryhyperoxiaoxygen oscillations |
| spellingShingle | Akos Tiboldi Eva Hunyadi-Gulyas Peter Wohlrab Johannes A. Schmid Klaus Markstaller Klaus Ulrich Klein Verena Tretter Effects of Hyperoxia and Hyperoxic Oscillations on the Proteome of Murine Lung Microvascular Endothelium Antioxidants acute respiratory distress syndrome hyperoxic acute lung injury lung microvascular endothelial cells ventilator-induced lung injury hyperoxia oxygen oscillations |
| title | Effects of Hyperoxia and Hyperoxic Oscillations on the Proteome of Murine Lung Microvascular Endothelium |
| title_full | Effects of Hyperoxia and Hyperoxic Oscillations on the Proteome of Murine Lung Microvascular Endothelium |
| title_fullStr | Effects of Hyperoxia and Hyperoxic Oscillations on the Proteome of Murine Lung Microvascular Endothelium |
| title_full_unstemmed | Effects of Hyperoxia and Hyperoxic Oscillations on the Proteome of Murine Lung Microvascular Endothelium |
| title_short | Effects of Hyperoxia and Hyperoxic Oscillations on the Proteome of Murine Lung Microvascular Endothelium |
| title_sort | effects of hyperoxia and hyperoxic oscillations on the proteome of murine lung microvascular endothelium |
| topic | acute respiratory distress syndrome hyperoxic acute lung injury lung microvascular endothelial cells ventilator-induced lung injury hyperoxia oxygen oscillations |
| url | https://www.mdpi.com/2076-3921/11/12/2349 |
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