Decellularized Adipose Tissue Hydrogel Promotes Bone Regeneration in Critical-Sized Mouse Femoral Defect Model
Critical-sized bone defects fail to heal and often cause non-union. Standard treatments employ autologous bone grafting, which can cause donor tissue loss/pain. Although several scaffold types can enhance bone regeneration, multiple factors limit their level of success. To address this issue, this s...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-09-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fbioe.2019.00211/full |
| _version_ | 1818275267434512384 |
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| author | Omair A. Mohiuddin Brett Campbell J. Nick Poche Michelle Ma Michelle Ma Emma Rogers Dina Gaupp Mark A. A. Harrison Bruce A. Bunnell Daniel J. Hayes Jeffrey M. Gimble Jeffrey M. Gimble |
| author_facet | Omair A. Mohiuddin Brett Campbell J. Nick Poche Michelle Ma Michelle Ma Emma Rogers Dina Gaupp Mark A. A. Harrison Bruce A. Bunnell Daniel J. Hayes Jeffrey M. Gimble Jeffrey M. Gimble |
| author_sort | Omair A. Mohiuddin |
| collection | DOAJ |
| description | Critical-sized bone defects fail to heal and often cause non-union. Standard treatments employ autologous bone grafting, which can cause donor tissue loss/pain. Although several scaffold types can enhance bone regeneration, multiple factors limit their level of success. To address this issue, this study evaluated a novel decellularized human adipose tissue (DAT) hydrogel as an alternative. In this study, DAT hydrogel alone, or in combination with adipose-derived stromal/stem cells (ASC), osteo-induced ASCs (OIASC), and hydroxyapatite were tested for their ability to mediate repair of a critical-sized (3 mm) femoral defect created in C57BL/6 mice. Micro-computed tomography results showed that all DAT hydrogel treated groups significantly enhanced bone regeneration, with OIASC + hydroxyapatite treated group displaying the most robust bone regeneration. Histological analyses revealed that all treatments resulted in significantly higher tissue areas with the relative mineralized tissue area significantly increased at 12 weeks; however, cartilaginous content was lowest among treatment groups with OIASC. Immunohistochemical analyses showed that DAT hydrogel enhanced collagen I and osteopontin expression, while the addition of OIASCs to the hydrogel reduced collagen II levels. Thus, DAT hydrogel promotes bone regeneration in a critical-sized femoral defect model that is further enhanced in the presence of OIASCs and hydroxyapatite. |
| first_indexed | 2024-12-12T22:27:02Z |
| format | Article |
| id | doaj.art-a84dcdc754334d22acd63d9267cdf06d |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-12-12T22:27:02Z |
| publishDate | 2019-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-a84dcdc754334d22acd63d9267cdf06d2022-12-22T00:09:43ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852019-09-01710.3389/fbioe.2019.00211484364Decellularized Adipose Tissue Hydrogel Promotes Bone Regeneration in Critical-Sized Mouse Femoral Defect ModelOmair A. Mohiuddin0Brett Campbell1J. Nick Poche2Michelle Ma3Michelle Ma4Emma Rogers5Dina Gaupp6Mark A. A. Harrison7Bruce A. Bunnell8Daniel J. Hayes9Jeffrey M. Gimble10Jeffrey M. Gimble11Center for Stem Cell Research and Regenerative Medicine, Tulane University School of Medicine, New Orleans, LA, United StatesSchool of Medicine, Tulane University, New Orleans, LA, United StatesSchool of Medicine, Louisiana State University, New Orleans, LA, United StatesCenter for Stem Cell Research and Regenerative Medicine, Tulane University School of Medicine, New Orleans, LA, United StatesObatala Sciences, New Orleans, LA, United StatesDepartment of Biomedical Engineering, Tulane University, New Orleans, LA, United StatesCenter for Stem Cell Research and Regenerative Medicine, Tulane University School of Medicine, New Orleans, LA, United StatesCenter for Stem Cell Research and Regenerative Medicine, Tulane University School of Medicine, New Orleans, LA, United StatesCenter for Stem Cell Research and Regenerative Medicine, Tulane University School of Medicine, New Orleans, LA, United StatesDepartment of Biomedical Engineering, Pennsylvania State University, State College, PA, United StatesCenter for Stem Cell Research and Regenerative Medicine, Tulane University School of Medicine, New Orleans, LA, United StatesLaCell LLC, New Orleans, LA, United StatesCritical-sized bone defects fail to heal and often cause non-union. Standard treatments employ autologous bone grafting, which can cause donor tissue loss/pain. Although several scaffold types can enhance bone regeneration, multiple factors limit their level of success. To address this issue, this study evaluated a novel decellularized human adipose tissue (DAT) hydrogel as an alternative. In this study, DAT hydrogel alone, or in combination with adipose-derived stromal/stem cells (ASC), osteo-induced ASCs (OIASC), and hydroxyapatite were tested for their ability to mediate repair of a critical-sized (3 mm) femoral defect created in C57BL/6 mice. Micro-computed tomography results showed that all DAT hydrogel treated groups significantly enhanced bone regeneration, with OIASC + hydroxyapatite treated group displaying the most robust bone regeneration. Histological analyses revealed that all treatments resulted in significantly higher tissue areas with the relative mineralized tissue area significantly increased at 12 weeks; however, cartilaginous content was lowest among treatment groups with OIASC. Immunohistochemical analyses showed that DAT hydrogel enhanced collagen I and osteopontin expression, while the addition of OIASCs to the hydrogel reduced collagen II levels. Thus, DAT hydrogel promotes bone regeneration in a critical-sized femoral defect model that is further enhanced in the presence of OIASCs and hydroxyapatite.https://www.frontiersin.org/article/10.3389/fbioe.2019.00211/fullcritical-sized femoral defectdecellularized adipose tissue hydrogeladipose derived stromal/stem cells (ASC)hydroxyapatitehistologyimmunohistochemistry |
| spellingShingle | Omair A. Mohiuddin Brett Campbell J. Nick Poche Michelle Ma Michelle Ma Emma Rogers Dina Gaupp Mark A. A. Harrison Bruce A. Bunnell Daniel J. Hayes Jeffrey M. Gimble Jeffrey M. Gimble Decellularized Adipose Tissue Hydrogel Promotes Bone Regeneration in Critical-Sized Mouse Femoral Defect Model Frontiers in Bioengineering and Biotechnology critical-sized femoral defect decellularized adipose tissue hydrogel adipose derived stromal/stem cells (ASC) hydroxyapatite histology immunohistochemistry |
| title | Decellularized Adipose Tissue Hydrogel Promotes Bone Regeneration in Critical-Sized Mouse Femoral Defect Model |
| title_full | Decellularized Adipose Tissue Hydrogel Promotes Bone Regeneration in Critical-Sized Mouse Femoral Defect Model |
| title_fullStr | Decellularized Adipose Tissue Hydrogel Promotes Bone Regeneration in Critical-Sized Mouse Femoral Defect Model |
| title_full_unstemmed | Decellularized Adipose Tissue Hydrogel Promotes Bone Regeneration in Critical-Sized Mouse Femoral Defect Model |
| title_short | Decellularized Adipose Tissue Hydrogel Promotes Bone Regeneration in Critical-Sized Mouse Femoral Defect Model |
| title_sort | decellularized adipose tissue hydrogel promotes bone regeneration in critical sized mouse femoral defect model |
| topic | critical-sized femoral defect decellularized adipose tissue hydrogel adipose derived stromal/stem cells (ASC) hydroxyapatite histology immunohistochemistry |
| url | https://www.frontiersin.org/article/10.3389/fbioe.2019.00211/full |
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