miR-505-5p alleviates acute rejection of liver transplantation by inhibiting Myd88 and inducing M2 polarizationof Kupffer cells
The occurrence of acute rejection after liver transplantation seriously impairs the prognosis of patients. miRNA is involved in many physiological and pathological processes of the body, but the mechanism of miRNA action in liver transplantation is not completely clear. In this study, we discuss the...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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China Science Publishing & Media Ltd.
2022-08-01
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| Series: | Acta Biochimica et Biophysica Sinica |
| Subjects: | |
| Online Access: | https://www.sciengine.com/doi/10.3724/abbs.2022100 |
| _version_ | 1797635363501506560 |
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| author | Chai Hao Lei Zilun Liu Yanyao Gong Junhua Cao Zhenrui Huang Zuotian Yang Hang Wu Zhongjun |
| author_facet | Chai Hao Lei Zilun Liu Yanyao Gong Junhua Cao Zhenrui Huang Zuotian Yang Hang Wu Zhongjun |
| author_sort | Chai Hao |
| collection | DOAJ |
| description | The occurrence of acute rejection after liver transplantation seriously impairs the prognosis of patients. miRNA is involved in many physiological and pathological processes of the body, but the mechanism of miRNA action in liver transplantation is not completely clear. In this study, we discuss the role of miR-505-5p in acute rejection after liver transplantation and its putative regulating mechanism. We construct an allogeneic rat liver transplantation model, observe the morphological and pathological changes in liver tissue, detect the expression levels of Myd88, miR-505-5p, IL-10 and TNF-α, and confirm that Myd88 is one of the direct targets of miR-505. The effects of miR-505-5p on the Myd88/TRAF6/NF-κB and MAPK pathways are detected both in vitro and in vivo, and the standard markers of Kupffer cell M1/M2 polarization are also detected. The results of qRT-PCR experiments show that miR-505-5p has a downward trend in rats with acute rejection. Western blot analysis reveals that over-expression of miR-505-5p induces the reduction of NF-κB and MAPK pathways both in vitro and in vivo. The role of miR-505-5p in alleviating acute rejection after transplantation may be accomplished by inducing M2-type polarization of Kupffer cells. In conclusion, we find that miR-505-5p alleviates acute rejection of liver transplantation by inducing M2 polarization of macrophages via the Myd88/TRAF6 axis, which suggests a potential strategy based on miRNAs in the follow-up treatment of liver transplantation. |
| first_indexed | 2024-03-11T12:21:02Z |
| format | Article |
| id | doaj.art-a851ea21116142bbbc51732b6dafc8b7 |
| institution | Directory Open Access Journal |
| issn | 1672-9145 |
| language | English |
| last_indexed | 2024-03-11T12:21:02Z |
| publishDate | 2022-08-01 |
| publisher | China Science Publishing & Media Ltd. |
| record_format | Article |
| series | Acta Biochimica et Biophysica Sinica |
| spelling | doaj.art-a851ea21116142bbbc51732b6dafc8b72023-11-07T01:01:08ZengChina Science Publishing & Media Ltd.Acta Biochimica et Biophysica Sinica1672-91452022-08-01541148115810.3724/abbs.202210020d259ccmiR-505-5p alleviates acute rejection of liver transplantation by inhibiting Myd88 and inducing M2 polarizationof Kupffer cellsChai Hao0Lei Zilun1Liu Yanyao2Gong Junhua3Cao Zhenrui4Huang Zuotian5Yang Hang6Wu Zhongjun7[][][][][][][][]The occurrence of acute rejection after liver transplantation seriously impairs the prognosis of patients. miRNA is involved in many physiological and pathological processes of the body, but the mechanism of miRNA action in liver transplantation is not completely clear. In this study, we discuss the role of miR-505-5p in acute rejection after liver transplantation and its putative regulating mechanism. We construct an allogeneic rat liver transplantation model, observe the morphological and pathological changes in liver tissue, detect the expression levels of Myd88, miR-505-5p, IL-10 and TNF-α, and confirm that Myd88 is one of the direct targets of miR-505. The effects of miR-505-5p on the Myd88/TRAF6/NF-κB and MAPK pathways are detected both in vitro and in vivo, and the standard markers of Kupffer cell M1/M2 polarization are also detected. The results of qRT-PCR experiments show that miR-505-5p has a downward trend in rats with acute rejection. Western blot analysis reveals that over-expression of miR-505-5p induces the reduction of NF-κB and MAPK pathways both in vitro and in vivo. The role of miR-505-5p in alleviating acute rejection after transplantation may be accomplished by inducing M2-type polarization of Kupffer cells. In conclusion, we find that miR-505-5p alleviates acute rejection of liver transplantation by inducing M2 polarization of macrophages via the Myd88/TRAF6 axis, which suggests a potential strategy based on miRNAs in the follow-up treatment of liver transplantation.https://www.sciengine.com/doi/10.3724/abbs.2022100immune toleranceliver transplantationmacrophagesmiR-505-5pMyd88 |
| spellingShingle | Chai Hao Lei Zilun Liu Yanyao Gong Junhua Cao Zhenrui Huang Zuotian Yang Hang Wu Zhongjun miR-505-5p alleviates acute rejection of liver transplantation by inhibiting Myd88 and inducing M2 polarizationof Kupffer cells Acta Biochimica et Biophysica Sinica immune tolerance liver transplantation macrophages miR-505-5p Myd88 |
| title | miR-505-5p alleviates acute rejection of liver transplantation by inhibiting Myd88 and inducing M2 polarizationof Kupffer cells |
| title_full | miR-505-5p alleviates acute rejection of liver transplantation by inhibiting Myd88 and inducing M2 polarizationof Kupffer cells |
| title_fullStr | miR-505-5p alleviates acute rejection of liver transplantation by inhibiting Myd88 and inducing M2 polarizationof Kupffer cells |
| title_full_unstemmed | miR-505-5p alleviates acute rejection of liver transplantation by inhibiting Myd88 and inducing M2 polarizationof Kupffer cells |
| title_short | miR-505-5p alleviates acute rejection of liver transplantation by inhibiting Myd88 and inducing M2 polarizationof Kupffer cells |
| title_sort | mir 505 5p alleviates acute rejection of liver transplantation by inhibiting myd88 and inducing m2 polarizationof kupffer cells |
| topic | immune tolerance liver transplantation macrophages miR-505-5p Myd88 |
| url | https://www.sciengine.com/doi/10.3724/abbs.2022100 |
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