Responses of Potato (<i>Solanum tuberosum</i> L.) Breeding Lines to Osmotic Stress Induced in In Vitro Shoot Culture
In vitro experiments were conducted to study the responses of potato (<i>Solanum tuberosum</i> L.) genotypes to osmotic stress. In vitro shoot cultures of 27 breeding lines and their drought-tolerant parents (referent lines: C103 and C107) were tested under osmotic stress induced by addi...
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MDPI AG
2022-07-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/8/7/591 |
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| author | Alexandra Hanász Judit Dobránszki Nóra Mendler-Drienyovszki László Zsombik Katalin Magyar-Tábori |
| author_facet | Alexandra Hanász Judit Dobránszki Nóra Mendler-Drienyovszki László Zsombik Katalin Magyar-Tábori |
| author_sort | Alexandra Hanász |
| collection | DOAJ |
| description | In vitro experiments were conducted to study the responses of potato (<i>Solanum tuberosum</i> L.) genotypes to osmotic stress. In vitro shoot cultures of 27 breeding lines and their drought-tolerant parents (referent lines: C103 and C107) were tested under osmotic stress induced by addition of PEG 6000 (Mw = 6000; 5.0, 7.5, 10.0%, <i>w</i>/<i>v</i>), D-mannitol (0.1, 0.2, 0.3 M) and PEG 600 (Mw = 600; 2.5, 5.0, 7.5%, <i>w</i>/<i>v</i>) to the Murashige-Skoog medium. Stress index (SI) was calculated from shoot length (SL) and root length (RL), root numbers (RN) and the rate of surviving shoots (SR) (SI<sub>SL;RL;RN;SR</sub> = Parameter<sub>SL;RL;RN;SR</sub> of treated shoots/Parameter<sub>SL;RL;RN;SR</sub> of control shoots × 100) to compare genotypes. In the average of each breeding line and concentration, the osmotic agents resulted in SI values of 40.1, 60.8, 82.6 and 76.0 for SI<sub>SL</sub>, SI<sub>RL</sub>, SI<sub>RN</sub> and SI<sub>SR</sub>, respectively. In general, all SI values of C103 and SI<sub>RL,RN</sub> of C107 were significantly higher than those of the breeding lines. Nine breeding lines were found to be promising based on their final ranking. According to the results, 7.5% and 10% PEG 6000 or 0.2 M and 0.3 M D-mannitol treatments proved to be suitable for the selection of osmotic stress-tolerant genotypes. |
| first_indexed | 2024-03-09T10:18:34Z |
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| id | doaj.art-a85459e3c47e4ac398e9570fecfbd58a |
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| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-09T10:18:34Z |
| publishDate | 2022-07-01 |
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| spelling | doaj.art-a85459e3c47e4ac398e9570fecfbd58a2023-12-01T22:13:03ZengMDPI AGHorticulturae2311-75242022-07-018759110.3390/horticulturae8070591Responses of Potato (<i>Solanum tuberosum</i> L.) Breeding Lines to Osmotic Stress Induced in In Vitro Shoot CultureAlexandra Hanász0Judit Dobránszki1Nóra Mendler-Drienyovszki2László Zsombik3Katalin Magyar-Tábori4Kerpely Kálmán Doctoral School of Crop Production and Horticultural Sciences, University of Debrecen, Böszörményi St. 138, 4032 Debrecen, HungaryCentre for Agricultural Genomics and Biotechnology, Faculty of the Agricultural and Food Science and Environmental Management, University of Debrecen, P.O. Box 12, 4400 Nyiregyhaza, HungaryResearch Institute of Nyíregyháza, Institutes for Agricultural Research and Educational Farm (IAREF), University of Debrecen, P.O. Box 12, 4400 Nyiregyhaza, HungaryResearch Institute of Nyíregyháza, Institutes for Agricultural Research and Educational Farm (IAREF), University of Debrecen, P.O. Box 12, 4400 Nyiregyhaza, HungaryResearch Institute of Nyíregyháza, Institutes for Agricultural Research and Educational Farm (IAREF), University of Debrecen, P.O. Box 12, 4400 Nyiregyhaza, HungaryIn vitro experiments were conducted to study the responses of potato (<i>Solanum tuberosum</i> L.) genotypes to osmotic stress. In vitro shoot cultures of 27 breeding lines and their drought-tolerant parents (referent lines: C103 and C107) were tested under osmotic stress induced by addition of PEG 6000 (Mw = 6000; 5.0, 7.5, 10.0%, <i>w</i>/<i>v</i>), D-mannitol (0.1, 0.2, 0.3 M) and PEG 600 (Mw = 600; 2.5, 5.0, 7.5%, <i>w</i>/<i>v</i>) to the Murashige-Skoog medium. Stress index (SI) was calculated from shoot length (SL) and root length (RL), root numbers (RN) and the rate of surviving shoots (SR) (SI<sub>SL;RL;RN;SR</sub> = Parameter<sub>SL;RL;RN;SR</sub> of treated shoots/Parameter<sub>SL;RL;RN;SR</sub> of control shoots × 100) to compare genotypes. In the average of each breeding line and concentration, the osmotic agents resulted in SI values of 40.1, 60.8, 82.6 and 76.0 for SI<sub>SL</sub>, SI<sub>RL</sub>, SI<sub>RN</sub> and SI<sub>SR</sub>, respectively. In general, all SI values of C103 and SI<sub>RL,RN</sub> of C107 were significantly higher than those of the breeding lines. Nine breeding lines were found to be promising based on their final ranking. According to the results, 7.5% and 10% PEG 6000 or 0.2 M and 0.3 M D-mannitol treatments proved to be suitable for the selection of osmotic stress-tolerant genotypes.https://www.mdpi.com/2311-7524/8/7/591polyethylene glycolD-mannitoltissue culturestress indexmorphological traits |
| spellingShingle | Alexandra Hanász Judit Dobránszki Nóra Mendler-Drienyovszki László Zsombik Katalin Magyar-Tábori Responses of Potato (<i>Solanum tuberosum</i> L.) Breeding Lines to Osmotic Stress Induced in In Vitro Shoot Culture Horticulturae polyethylene glycol D-mannitol tissue culture stress index morphological traits |
| title | Responses of Potato (<i>Solanum tuberosum</i> L.) Breeding Lines to Osmotic Stress Induced in In Vitro Shoot Culture |
| title_full | Responses of Potato (<i>Solanum tuberosum</i> L.) Breeding Lines to Osmotic Stress Induced in In Vitro Shoot Culture |
| title_fullStr | Responses of Potato (<i>Solanum tuberosum</i> L.) Breeding Lines to Osmotic Stress Induced in In Vitro Shoot Culture |
| title_full_unstemmed | Responses of Potato (<i>Solanum tuberosum</i> L.) Breeding Lines to Osmotic Stress Induced in In Vitro Shoot Culture |
| title_short | Responses of Potato (<i>Solanum tuberosum</i> L.) Breeding Lines to Osmotic Stress Induced in In Vitro Shoot Culture |
| title_sort | responses of potato i solanum tuberosum i l breeding lines to osmotic stress induced in in vitro shoot culture |
| topic | polyethylene glycol D-mannitol tissue culture stress index morphological traits |
| url | https://www.mdpi.com/2311-7524/8/7/591 |
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