Protein Extraction, Precipitation, and Recovery from <em>Chlorella sorokiniana</em> Using Mechanochemical Methods
Protein extraction, precipitation, and recovery methods were evaluated by this study using a green alga—<i>Chlorella sorokiniana</i>. A mechanochemical cell disruption process was applied to facilitate protein extraction from microalgal biomass. Optimization of the mechanochemical proces...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-06-01
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| Series: | Energies |
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| Online Access: | https://www.mdpi.com/1996-1073/16/12/4809 |
| _version_ | 1797595055643426816 |
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| author | Ashley Cutshaw Henry Frost Sibel Uludag-Demirer Yan Liu Wei Liao |
| author_facet | Ashley Cutshaw Henry Frost Sibel Uludag-Demirer Yan Liu Wei Liao |
| author_sort | Ashley Cutshaw |
| collection | DOAJ |
| description | Protein extraction, precipitation, and recovery methods were evaluated by this study using a green alga—<i>Chlorella sorokiniana</i>. A mechanochemical cell disruption process was applied to facilitate protein extraction from microalgal biomass. Optimization of the mechanochemical process resulted in milling conditions that achieved a protein extraction of 52.7 ± 6.45%. The consequent acid precipitation method was optimized to recover 98.7% of proteins from the microalgal slurry. The measured protein content of the protein isolate was 41.4% <i>w</i>/<i>w</i>. These results indicate that the precipitation method is successful at recovering the extracted proteins in the algal slurry; however, the removal of non-protein solids during centrifugation and pH adjustment is not complete. The energy balance analysis elucidated that the energy demand of the protein extraction and recovery operation, at 0.83 MJ/kg dry algal biomass, is much lower than previous studies using high-pressure homogenization and membrane filtration. This study concludes that mechanochemical protein extraction and recovery is an effective, low-energy processing method, which could be used by algal biorefineries to prepare algal proteins for value-added chemical production as well as to make algal carbohydrates and lipids in the residual biomass more accessible for biofuel production. |
| first_indexed | 2024-03-11T02:31:00Z |
| format | Article |
| id | doaj.art-a85c0ae0790844759c50714b4da1fe35 |
| institution | Directory Open Access Journal |
| issn | 1996-1073 |
| language | English |
| last_indexed | 2024-03-11T02:31:00Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Energies |
| spelling | doaj.art-a85c0ae0790844759c50714b4da1fe352023-11-18T10:14:24ZengMDPI AGEnergies1996-10732023-06-011612480910.3390/en16124809Protein Extraction, Precipitation, and Recovery from <em>Chlorella sorokiniana</em> Using Mechanochemical MethodsAshley Cutshaw0Henry Frost1Sibel Uludag-Demirer2Yan Liu3Wei Liao4Biosystems and Agricultural Engineering, Michigan State University, East Lansing, MI 48824, USABiosystems and Agricultural Engineering, Michigan State University, East Lansing, MI 48824, USABiosystems and Agricultural Engineering, Michigan State University, East Lansing, MI 48824, USABiosystems and Agricultural Engineering, Michigan State University, East Lansing, MI 48824, USABiosystems and Agricultural Engineering, Michigan State University, East Lansing, MI 48824, USAProtein extraction, precipitation, and recovery methods were evaluated by this study using a green alga—<i>Chlorella sorokiniana</i>. A mechanochemical cell disruption process was applied to facilitate protein extraction from microalgal biomass. Optimization of the mechanochemical process resulted in milling conditions that achieved a protein extraction of 52.7 ± 6.45%. The consequent acid precipitation method was optimized to recover 98.7% of proteins from the microalgal slurry. The measured protein content of the protein isolate was 41.4% <i>w</i>/<i>w</i>. These results indicate that the precipitation method is successful at recovering the extracted proteins in the algal slurry; however, the removal of non-protein solids during centrifugation and pH adjustment is not complete. The energy balance analysis elucidated that the energy demand of the protein extraction and recovery operation, at 0.83 MJ/kg dry algal biomass, is much lower than previous studies using high-pressure homogenization and membrane filtration. This study concludes that mechanochemical protein extraction and recovery is an effective, low-energy processing method, which could be used by algal biorefineries to prepare algal proteins for value-added chemical production as well as to make algal carbohydrates and lipids in the residual biomass more accessible for biofuel production.https://www.mdpi.com/1996-1073/16/12/4809ball millproteinmicroalgaefractionationmass and energy balance |
| spellingShingle | Ashley Cutshaw Henry Frost Sibel Uludag-Demirer Yan Liu Wei Liao Protein Extraction, Precipitation, and Recovery from <em>Chlorella sorokiniana</em> Using Mechanochemical Methods Energies ball mill protein microalgae fractionation mass and energy balance |
| title | Protein Extraction, Precipitation, and Recovery from <em>Chlorella sorokiniana</em> Using Mechanochemical Methods |
| title_full | Protein Extraction, Precipitation, and Recovery from <em>Chlorella sorokiniana</em> Using Mechanochemical Methods |
| title_fullStr | Protein Extraction, Precipitation, and Recovery from <em>Chlorella sorokiniana</em> Using Mechanochemical Methods |
| title_full_unstemmed | Protein Extraction, Precipitation, and Recovery from <em>Chlorella sorokiniana</em> Using Mechanochemical Methods |
| title_short | Protein Extraction, Precipitation, and Recovery from <em>Chlorella sorokiniana</em> Using Mechanochemical Methods |
| title_sort | protein extraction precipitation and recovery from em chlorella sorokiniana em using mechanochemical methods |
| topic | ball mill protein microalgae fractionation mass and energy balance |
| url | https://www.mdpi.com/1996-1073/16/12/4809 |
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