The Effect of Polyphenol Extract from Rosa Roxburghii Fruit on Plasma Metabolome and Gut Microbiota in Type 2 Diabetic Mice
Rosa roxburghii fruit is an underutilized functional food abundant in polyphenols. Polyphenols have been proved to have antidiabetic effects. This study investigates the effects of Rosa roxburghii fruit polyphenols extract (RPE) on plasma metabolites and gut microbiota composition in streptozotocin...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-06-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/11/12/1747 |
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| author | Hui Wang Zhaojun Chen Mei Wang Mingxiu Long Tingyuan Ren Chao Chen Xiaotong Dai Sheng Yang Shuming Tan |
| author_facet | Hui Wang Zhaojun Chen Mei Wang Mingxiu Long Tingyuan Ren Chao Chen Xiaotong Dai Sheng Yang Shuming Tan |
| author_sort | Hui Wang |
| collection | DOAJ |
| description | Rosa roxburghii fruit is an underutilized functional food abundant in polyphenols. Polyphenols have been proved to have antidiabetic effects. This study investigates the effects of Rosa roxburghii fruit polyphenols extract (RPE) on plasma metabolites and gut microbiota composition in streptozotocin (STZ)- and high-fat diet- induced type 2 diabetes using metabolomics and 16S rRNA gene sequencing. The induced diabetic mice were fed with 400 mg/kg body weight RPE for 8 weeks. RPE demonstrated hypoglycemic, hypolipidemic, and anti-inflammatory effects. Colonic oxidative stress biomarkers were also lowered by RPE. Besides, RPE decreased plasma ceramides and tyrosine levels and increased carnitine and phosphatidylinositols levels, indicating improved insulin resistance, lipid metabolism, and immune response. Furthermore, RPE decreased abundances of <i>Lachnospiraceae</i> and <i>Rikenellaceae</i> and increased abundances of <i>Erysipelotrichaceae</i> and <i>Faecalibaculum</i>. Metabolic function prediction of the gut microbiota by PICRUSt demonstrated that RPE downregulated the phosphotransferase system. Taken together, these findings demonstrated that RPE has the potential to prevent type 2 diabetes by regulating the plasma metabolites and gut microbes. |
| first_indexed | 2024-03-09T23:48:56Z |
| format | Article |
| id | doaj.art-a85d942ea58b4b23ab73d32382d30490 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-09T23:48:56Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-a85d942ea58b4b23ab73d32382d304902023-11-23T16:38:26ZengMDPI AGFoods2304-81582022-06-011112174710.3390/foods11121747The Effect of Polyphenol Extract from Rosa Roxburghii Fruit on Plasma Metabolome and Gut Microbiota in Type 2 Diabetic MiceHui Wang0Zhaojun Chen1Mei Wang2Mingxiu Long3Tingyuan Ren4Chao Chen5Xiaotong Dai6Sheng Yang7Shuming Tan8Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences/Institute of Agro-Bioengineering, Guizhou University, Guiyang 550025, ChinaInstitute of Biotechnology, Guizhou Academy of Agricultural Sciences, Guiyang 550025, ChinaInstitute of Biotechnology, Guizhou Academy of Agricultural Sciences, Guiyang 550025, ChinaGuizhou Research Institute of Modern Agricultural Development, Guizhou Academy of Agricultural Sciences, Guiyang 550025, ChinaKey Laboratory of Agricultural and Animal Products Storage and Processing of Guizhou Province, College of Liquor and Food Engineering, Guizhou University, Guiyang 550025, ChinaKey Laboratory of Agricultural and Animal Products Storage and Processing of Guizhou Province, College of Liquor and Food Engineering, Guizhou University, Guiyang 550025, ChinaKey Laboratory of Agricultural and Animal Products Storage and Processing of Guizhou Province, College of Liquor and Food Engineering, Guizhou University, Guiyang 550025, ChinaKey Laboratory of Agricultural and Animal Products Storage and Processing of Guizhou Province, College of Liquor and Food Engineering, Guizhou University, Guiyang 550025, ChinaKey Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences/Institute of Agro-Bioengineering, Guizhou University, Guiyang 550025, ChinaRosa roxburghii fruit is an underutilized functional food abundant in polyphenols. Polyphenols have been proved to have antidiabetic effects. This study investigates the effects of Rosa roxburghii fruit polyphenols extract (RPE) on plasma metabolites and gut microbiota composition in streptozotocin (STZ)- and high-fat diet- induced type 2 diabetes using metabolomics and 16S rRNA gene sequencing. The induced diabetic mice were fed with 400 mg/kg body weight RPE for 8 weeks. RPE demonstrated hypoglycemic, hypolipidemic, and anti-inflammatory effects. Colonic oxidative stress biomarkers were also lowered by RPE. Besides, RPE decreased plasma ceramides and tyrosine levels and increased carnitine and phosphatidylinositols levels, indicating improved insulin resistance, lipid metabolism, and immune response. Furthermore, RPE decreased abundances of <i>Lachnospiraceae</i> and <i>Rikenellaceae</i> and increased abundances of <i>Erysipelotrichaceae</i> and <i>Faecalibaculum</i>. Metabolic function prediction of the gut microbiota by PICRUSt demonstrated that RPE downregulated the phosphotransferase system. Taken together, these findings demonstrated that RPE has the potential to prevent type 2 diabetes by regulating the plasma metabolites and gut microbes.https://www.mdpi.com/2304-8158/11/12/1747Rosa roxburghii fruittype 2 diabetesplasma metabolomegut microbiota |
| spellingShingle | Hui Wang Zhaojun Chen Mei Wang Mingxiu Long Tingyuan Ren Chao Chen Xiaotong Dai Sheng Yang Shuming Tan The Effect of Polyphenol Extract from Rosa Roxburghii Fruit on Plasma Metabolome and Gut Microbiota in Type 2 Diabetic Mice Foods Rosa roxburghii fruit type 2 diabetes plasma metabolome gut microbiota |
| title | The Effect of Polyphenol Extract from Rosa Roxburghii Fruit on Plasma Metabolome and Gut Microbiota in Type 2 Diabetic Mice |
| title_full | The Effect of Polyphenol Extract from Rosa Roxburghii Fruit on Plasma Metabolome and Gut Microbiota in Type 2 Diabetic Mice |
| title_fullStr | The Effect of Polyphenol Extract from Rosa Roxburghii Fruit on Plasma Metabolome and Gut Microbiota in Type 2 Diabetic Mice |
| title_full_unstemmed | The Effect of Polyphenol Extract from Rosa Roxburghii Fruit on Plasma Metabolome and Gut Microbiota in Type 2 Diabetic Mice |
| title_short | The Effect of Polyphenol Extract from Rosa Roxburghii Fruit on Plasma Metabolome and Gut Microbiota in Type 2 Diabetic Mice |
| title_sort | effect of polyphenol extract from rosa roxburghii fruit on plasma metabolome and gut microbiota in type 2 diabetic mice |
| topic | Rosa roxburghii fruit type 2 diabetes plasma metabolome gut microbiota |
| url | https://www.mdpi.com/2304-8158/11/12/1747 |
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