Three-Dimensional PLGA Nanofiber-Based Microchip for High-Efficiency Cancer Cell Capture
A 3D network capture substrate based on poly(lactic-co-glycolic acid) (PLGA) nanofibers was studied and successfully used for high-efficiency cancer cell capture. The arc-shaped glass micropillars were prepared by chemical wet etching and soft lithography. PLGA nanofibers were coupled with micropill...
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MDPI AG
2023-04-01
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| Series: | Materials |
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| Online Access: | https://www.mdpi.com/1996-1944/16/8/3065 |
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| author | Mengting Qi Meilin Ruan Jinjin Liang Zhengtao Zhang Chaohui Chen Yiping Cao Rongxiang He |
| author_facet | Mengting Qi Meilin Ruan Jinjin Liang Zhengtao Zhang Chaohui Chen Yiping Cao Rongxiang He |
| author_sort | Mengting Qi |
| collection | DOAJ |
| description | A 3D network capture substrate based on poly(lactic-co-glycolic acid) (PLGA) nanofibers was studied and successfully used for high-efficiency cancer cell capture. The arc-shaped glass micropillars were prepared by chemical wet etching and soft lithography. PLGA nanofibers were coupled with micropillars by electrospinning. Given the size effect of the microcolumn and PLGA nanofibers, a three-dimensional of micro-nanometer spatial network was prepared to form a network cell trapping substrate. After the modification of a specific anti-EpCAM antibody, MCF-7 cancer cells were captured successfully with a capture efficiency of 91%. Compared with the substrate composed of 2D nanofibers or nanoparticles, the developed 3D structure based on microcolumns and nanofibers had a greater contact probability between cells and the capture substrate, leading to a high capture efficiency. Cell capture based on this method can provide technical support for rare cells in peripheral blood detection, such as circulating tumor cells and circulating fetal nucleated red cells. |
| first_indexed | 2024-03-11T04:47:51Z |
| format | Article |
| id | doaj.art-a861ff0a2f894deb9384ff875d43e0ad |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-11T04:47:51Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Materials |
| spelling | doaj.art-a861ff0a2f894deb9384ff875d43e0ad2023-11-17T20:12:34ZengMDPI AGMaterials1996-19442023-04-01168306510.3390/ma16083065Three-Dimensional PLGA Nanofiber-Based Microchip for High-Efficiency Cancer Cell CaptureMengting Qi0Meilin Ruan1Jinjin Liang2Zhengtao Zhang3Chaohui Chen4Yiping Cao5Rongxiang He6Key Laboratory of Optoelectronic Chemical Materials and Devices of Ministry of Education, Institute of Interdisciplinary Research, Jianghan University, Wuhan 430056, ChinaKey Laboratory of Optoelectronic Chemical Materials and Devices of Ministry of Education, Institute of Interdisciplinary Research, Jianghan University, Wuhan 430056, ChinaKey Laboratory of Optoelectronic Chemical Materials and Devices of Ministry of Education, Institute of Interdisciplinary Research, Jianghan University, Wuhan 430056, ChinaKey Laboratory of Optoelectronic Chemical Materials and Devices of Ministry of Education, Institute of Interdisciplinary Research, Jianghan University, Wuhan 430056, ChinaKey Laboratory of Optoelectronic Chemical Materials and Devices of Ministry of Education, Institute of Interdisciplinary Research, Jianghan University, Wuhan 430056, ChinaKey Laboratory of Optoelectronic Chemical Materials and Devices of Ministry of Education, Institute of Interdisciplinary Research, Jianghan University, Wuhan 430056, ChinaKey Laboratory of Optoelectronic Chemical Materials and Devices of Ministry of Education, Institute of Interdisciplinary Research, Jianghan University, Wuhan 430056, ChinaA 3D network capture substrate based on poly(lactic-co-glycolic acid) (PLGA) nanofibers was studied and successfully used for high-efficiency cancer cell capture. The arc-shaped glass micropillars were prepared by chemical wet etching and soft lithography. PLGA nanofibers were coupled with micropillars by electrospinning. Given the size effect of the microcolumn and PLGA nanofibers, a three-dimensional of micro-nanometer spatial network was prepared to form a network cell trapping substrate. After the modification of a specific anti-EpCAM antibody, MCF-7 cancer cells were captured successfully with a capture efficiency of 91%. Compared with the substrate composed of 2D nanofibers or nanoparticles, the developed 3D structure based on microcolumns and nanofibers had a greater contact probability between cells and the capture substrate, leading to a high capture efficiency. Cell capture based on this method can provide technical support for rare cells in peripheral blood detection, such as circulating tumor cells and circulating fetal nucleated red cells.https://www.mdpi.com/1996-1944/16/8/3065cancer cellsnanofibersmicropillarmesoscopic interfacemicrochip |
| spellingShingle | Mengting Qi Meilin Ruan Jinjin Liang Zhengtao Zhang Chaohui Chen Yiping Cao Rongxiang He Three-Dimensional PLGA Nanofiber-Based Microchip for High-Efficiency Cancer Cell Capture Materials cancer cells nanofibers micropillar mesoscopic interface microchip |
| title | Three-Dimensional PLGA Nanofiber-Based Microchip for High-Efficiency Cancer Cell Capture |
| title_full | Three-Dimensional PLGA Nanofiber-Based Microchip for High-Efficiency Cancer Cell Capture |
| title_fullStr | Three-Dimensional PLGA Nanofiber-Based Microchip for High-Efficiency Cancer Cell Capture |
| title_full_unstemmed | Three-Dimensional PLGA Nanofiber-Based Microchip for High-Efficiency Cancer Cell Capture |
| title_short | Three-Dimensional PLGA Nanofiber-Based Microchip for High-Efficiency Cancer Cell Capture |
| title_sort | three dimensional plga nanofiber based microchip for high efficiency cancer cell capture |
| topic | cancer cells nanofibers micropillar mesoscopic interface microchip |
| url | https://www.mdpi.com/1996-1944/16/8/3065 |
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