Enhanced procedures for mosquito identification by MALDI-TOF MS
Abstract Background In the last decade, an innovative approach has emerged for arthropod identification based on matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). Increasing interest in applying the original technique for arthropod identification has led to...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2022-06-01
|
| Series: | Parasites & Vectors |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13071-022-05361-0 |
| _version_ | 1818235790598078464 |
|---|---|
| author | Roland Bamou Monique Melo Costa Adama Zan Diarra Ademir Jesus Martins Philippe Parola Lionel Almeras |
| author_facet | Roland Bamou Monique Melo Costa Adama Zan Diarra Ademir Jesus Martins Philippe Parola Lionel Almeras |
| author_sort | Roland Bamou |
| collection | DOAJ |
| description | Abstract Background In the last decade, an innovative approach has emerged for arthropod identification based on matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). Increasing interest in applying the original technique for arthropod identification has led to the development of a variety of procedures for sample preparation and selection of body parts, among others. However, the absence of a consensual strategy hampers direct inter-study comparisons. Moreover, these different procedures are confusing to new users. Establishing optimized procedures and standardized protocols for mosquito identification by MALDI-TOF MS is therefore a necessity, and would notably enable the sharing of reference MS databases. Here, we assess the optimal conditions for mosquito identification using MALDI-TOF MS profiling. Methods Three homogenization methods, two of which were manual and one automatic, were used on three distinct body parts (legs, thorax, head) of two mosquito laboratory strains, Anopheles coluzzii and Aedes aegypti, and the results evaluated. The reproducibility of MS profiles, identification rate with relevant scores and the suitability of procedures for high-throughput analyses were the main criteria for establishing optimized guidelines. Additionally, the consequences of blood-feeding and geographical origin were evaluated using both laboratory strains and field-collected mosquitoes. Results Relevant score values for mosquito identification were obtained for all the three body parts assayed using MALDI-TOF MS profiling; however, the thorax and legs were the most suitable specimens, independently of homogenization method or species. Although the manual homogenization methods were associated with a high rate of identification on the three body parts, this homogenization mode is not adaptable to the processing of a large number of samples. Therefore, the automatic homogenization procedure was selected as the reference homogenization method. Blood-feeding status did not hamper the identification of mosquito species, despite the presence of MS peaks from original blood in the MS profiles of the three body parts tested from both species. Finally, a significant improvement in identification scores was obtained for field-collected specimens when MS spectra of species from the same geographical area were added to the database. Conclusion The results of the current study establish guidelines for the selection of mosquito anatomic parts and modality of sample preparation (e.g. homogenization) for future specimen identification by MALDI-TOF MS profiling. These standardized operational protocols could be used as references for creating an international MS database. |
| first_indexed | 2024-12-12T11:59:34Z |
| format | Article |
| id | doaj.art-a865050ab9994f40919b0fa4b7bd7df9 |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-12-12T11:59:34Z |
| publishDate | 2022-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-a865050ab9994f40919b0fa4b7bd7df92022-12-22T00:25:09ZengBMCParasites & Vectors1756-33052022-06-0115111710.1186/s13071-022-05361-0Enhanced procedures for mosquito identification by MALDI-TOF MSRoland Bamou0Monique Melo Costa1Adama Zan Diarra2Ademir Jesus Martins3Philippe Parola4Lionel Almeras5Aix-Marseille Univ., IRD, SSA, AP-HM, VITROMEAix-Marseille Univ., IRD, SSA, AP-HM, VITROMEAix-Marseille Univ., IRD, SSA, AP-HM, VITROMELaboratório de Fisiologia e Controle de Artrópodes Vetores, Instituto Oswaldo Cruz (FIOCRUZ)Aix-Marseille Univ., IRD, SSA, AP-HM, VITROMEAix-Marseille Univ., IRD, SSA, AP-HM, VITROMEAbstract Background In the last decade, an innovative approach has emerged for arthropod identification based on matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). Increasing interest in applying the original technique for arthropod identification has led to the development of a variety of procedures for sample preparation and selection of body parts, among others. However, the absence of a consensual strategy hampers direct inter-study comparisons. Moreover, these different procedures are confusing to new users. Establishing optimized procedures and standardized protocols for mosquito identification by MALDI-TOF MS is therefore a necessity, and would notably enable the sharing of reference MS databases. Here, we assess the optimal conditions for mosquito identification using MALDI-TOF MS profiling. Methods Three homogenization methods, two of which were manual and one automatic, were used on three distinct body parts (legs, thorax, head) of two mosquito laboratory strains, Anopheles coluzzii and Aedes aegypti, and the results evaluated. The reproducibility of MS profiles, identification rate with relevant scores and the suitability of procedures for high-throughput analyses were the main criteria for establishing optimized guidelines. Additionally, the consequences of blood-feeding and geographical origin were evaluated using both laboratory strains and field-collected mosquitoes. Results Relevant score values for mosquito identification were obtained for all the three body parts assayed using MALDI-TOF MS profiling; however, the thorax and legs were the most suitable specimens, independently of homogenization method or species. Although the manual homogenization methods were associated with a high rate of identification on the three body parts, this homogenization mode is not adaptable to the processing of a large number of samples. Therefore, the automatic homogenization procedure was selected as the reference homogenization method. Blood-feeding status did not hamper the identification of mosquito species, despite the presence of MS peaks from original blood in the MS profiles of the three body parts tested from both species. Finally, a significant improvement in identification scores was obtained for field-collected specimens when MS spectra of species from the same geographical area were added to the database. Conclusion The results of the current study establish guidelines for the selection of mosquito anatomic parts and modality of sample preparation (e.g. homogenization) for future specimen identification by MALDI-TOF MS profiling. These standardized operational protocols could be used as references for creating an international MS database.https://doi.org/10.1186/s13071-022-05361-0Mosquitoes identificationMALDI-TOF MSOptimizationStandardization |
| spellingShingle | Roland Bamou Monique Melo Costa Adama Zan Diarra Ademir Jesus Martins Philippe Parola Lionel Almeras Enhanced procedures for mosquito identification by MALDI-TOF MS Parasites & Vectors Mosquitoes identification MALDI-TOF MS Optimization Standardization |
| title | Enhanced procedures for mosquito identification by MALDI-TOF MS |
| title_full | Enhanced procedures for mosquito identification by MALDI-TOF MS |
| title_fullStr | Enhanced procedures for mosquito identification by MALDI-TOF MS |
| title_full_unstemmed | Enhanced procedures for mosquito identification by MALDI-TOF MS |
| title_short | Enhanced procedures for mosquito identification by MALDI-TOF MS |
| title_sort | enhanced procedures for mosquito identification by maldi tof ms |
| topic | Mosquitoes identification MALDI-TOF MS Optimization Standardization |
| url | https://doi.org/10.1186/s13071-022-05361-0 |
| work_keys_str_mv | AT rolandbamou enhancedproceduresformosquitoidentificationbymalditofms AT moniquemelocosta enhancedproceduresformosquitoidentificationbymalditofms AT adamazandiarra enhancedproceduresformosquitoidentificationbymalditofms AT ademirjesusmartins enhancedproceduresformosquitoidentificationbymalditofms AT philippeparola enhancedproceduresformosquitoidentificationbymalditofms AT lionelalmeras enhancedproceduresformosquitoidentificationbymalditofms |