Isolation and Molecular Identification of Keratinase-Producing Bacteria from the Sludge of Qeshm Island
ABSTRACT Background and Objectives: Keratinase is an enzyme commonly used in the production of detergents, cosmetics, drugs, leather, and other industries. Considering the high cost of traditional methods for decomposition of feather, hair, hooves, nails, and wool that contain high levels...
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| Format: | Article |
| Language: | English |
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Golestan University of Medical Sciences
2018-05-01
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| Series: | Medical Laboratory Journal |
| Subjects: | |
| Online Access: | http://mlj.goums.ac.ir/article-1-1060-en.html |
| _version_ | 1818157478438764544 |
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| author | Maedeh Kiani Abri Monir Doudi Ali Mohammad Ahadi |
| author_facet | Maedeh Kiani Abri Monir Doudi Ali Mohammad Ahadi |
| author_sort | Maedeh Kiani Abri |
| collection | DOAJ |
| description | ABSTRACT
Background and Objectives: Keratinase is an enzyme commonly used in the production of detergents, cosmetics, drugs, leather, and other industries. Considering the high cost of traditional methods for decomposition of feather, hair, hooves, nails, and wool that contain high levels of keratin, their biodegradation with keratinase-producing bacteria can be a valuable solution. The present study aimed for isolation and molecular identification of keratinase-producing bacteria in Qeshm Island and Peyposht village in Iran.
Methods: Water and sludge samples from the Qeshm Island and Peyposht village were collected. The bacteria isolates were screened for keratinase production using the Lowry method. Effect of pH and temperature was assessed on the production of keratinase and on the growth of the isolates. Colony-polymerase chain reaction was used for molecular identification of the isolates.
Results: Bacillus berevis and Enterobacter cloacae were isolated in this study. Keratinase production in B. berevis was highest at pH 7.5 and 35 °C. In addition, the highest level of enzyme production by E. cloacae was observed at pH 7 and 37 °C.
Conclusion: It seems that the bacterial strains isolated from sludge in the study area have relatively favorable keratinase production capacity.
Keywords: Bacteria, Colony PCR, Identification, Keratinolytic protein, Sewage. |
| first_indexed | 2024-12-11T15:14:50Z |
| format | Article |
| id | doaj.art-a869be47bb9a4c3a873ee92ac8f2b305 |
| institution | Directory Open Access Journal |
| issn | 2538-4449 |
| language | English |
| last_indexed | 2024-12-11T15:14:50Z |
| publishDate | 2018-05-01 |
| publisher | Golestan University of Medical Sciences |
| record_format | Article |
| series | Medical Laboratory Journal |
| spelling | doaj.art-a869be47bb9a4c3a873ee92ac8f2b3052022-12-22T01:00:37ZengGolestan University of Medical SciencesMedical Laboratory Journal2538-44492018-05-011233440Isolation and Molecular Identification of Keratinase-Producing Bacteria from the Sludge of Qeshm IslandMaedeh Kiani Abri0Monir Doudi1Ali Mohammad Ahadi2 Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran Department of Genetics, Shahrekord University Branch, Shahrekord, Iran ABSTRACT Background and Objectives: Keratinase is an enzyme commonly used in the production of detergents, cosmetics, drugs, leather, and other industries. Considering the high cost of traditional methods for decomposition of feather, hair, hooves, nails, and wool that contain high levels of keratin, their biodegradation with keratinase-producing bacteria can be a valuable solution. The present study aimed for isolation and molecular identification of keratinase-producing bacteria in Qeshm Island and Peyposht village in Iran. Methods: Water and sludge samples from the Qeshm Island and Peyposht village were collected. The bacteria isolates were screened for keratinase production using the Lowry method. Effect of pH and temperature was assessed on the production of keratinase and on the growth of the isolates. Colony-polymerase chain reaction was used for molecular identification of the isolates. Results: Bacillus berevis and Enterobacter cloacae were isolated in this study. Keratinase production in B. berevis was highest at pH 7.5 and 35 °C. In addition, the highest level of enzyme production by E. cloacae was observed at pH 7 and 37 °C. Conclusion: It seems that the bacterial strains isolated from sludge in the study area have relatively favorable keratinase production capacity. Keywords: Bacteria, Colony PCR, Identification, Keratinolytic protein, Sewage.http://mlj.goums.ac.ir/article-1-1060-en.htmlbacteriacolony pcridentificationkeratinolytic proteinsewage |
| spellingShingle | Maedeh Kiani Abri Monir Doudi Ali Mohammad Ahadi Isolation and Molecular Identification of Keratinase-Producing Bacteria from the Sludge of Qeshm Island Medical Laboratory Journal bacteria colony pcr identification keratinolytic protein sewage |
| title | Isolation and Molecular Identification of Keratinase-Producing Bacteria from the Sludge of Qeshm Island |
| title_full | Isolation and Molecular Identification of Keratinase-Producing Bacteria from the Sludge of Qeshm Island |
| title_fullStr | Isolation and Molecular Identification of Keratinase-Producing Bacteria from the Sludge of Qeshm Island |
| title_full_unstemmed | Isolation and Molecular Identification of Keratinase-Producing Bacteria from the Sludge of Qeshm Island |
| title_short | Isolation and Molecular Identification of Keratinase-Producing Bacteria from the Sludge of Qeshm Island |
| title_sort | isolation and molecular identification of keratinase producing bacteria from the sludge of qeshm island |
| topic | bacteria colony pcr identification keratinolytic protein sewage |
| url | http://mlj.goums.ac.ir/article-1-1060-en.html |
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