Serotype distribution and resistance genes associated with macrolide and fluoroquinolone resistance in Streptococcus agalactiae isolates from a hospital in southern Taiwan
Background: Antimicrobial resistance of Streptococcus agalactiae (Group B Streptococcus, GBS) has been emerging worldwide. We aimed to examine the correlation of drug-resistant genes with serotypes and with the mutations of the quinolone resistance-determining region (QRDR) in GBS isolates. Methods...
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| Format: | Article |
| Language: | English |
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Elsevier
2015-06-01
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| Series: | Biomedical Journal |
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| Online Access: | http://www.biomedj.org/article.asp?issn=2319-4170;year=2015;volume=38;issue=3;spage=215;epage=220;aulast=Wang |
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| author | Ying-Hsiang Wang Chyi-Liang Chen Jiun-Nub Hou Yi-Rou Wang Ting-Yu Lin Mei-Hei Wang Tsung-Han Yang Chishih Chu Cheng-Hsun Chiu |
| author_facet | Ying-Hsiang Wang Chyi-Liang Chen Jiun-Nub Hou Yi-Rou Wang Ting-Yu Lin Mei-Hei Wang Tsung-Han Yang Chishih Chu Cheng-Hsun Chiu |
| author_sort | Ying-Hsiang Wang |
| collection | DOAJ |
| description | Background: Antimicrobial resistance of Streptococcus agalactiae (Group B Streptococcus, GBS) has been emerging worldwide. We aimed to examine the correlation of drug-resistant genes with serotypes and with the mutations of the quinolone resistance-determining region (QRDR) in GBS isolates.
Methods: A total of 323 human GBS isolates were collected from a hospital in southern Taiwan. Laboratory investigation included serotyping by a multiplex polymerase chain reaction (PCR) method, antimicrobial susceptibility testing by a disc diffusion method, and mechanism analysis of the resistance to macrolides and fluoroquinolones by PCR and sequencing methods.
Results: Multiplex PCR showed that the most prevalent serotypes were Ib, III, V, and VI, mostly isolated from urine. The ermB gene was highly prevalent in serotypes Ib and V and was associated with clindamycin and macrolide resistance. GBS with a serine-to-leucine mutation at codon 81 in GyrA and with a serine-to-phenylalanine or -tyrosine mutation at codon 79 in ParC had a higher minimum inhibitory concentration of levofloxacin than isolates with only an aspartic acid-to-tyrosine mutation at codon 83 (>32 μg/ml vs. 16 μg/ml) in GyrA.
Conclusions: The most prevalent GBS serotypes were Ib, III, V, and VI. The ermB and mefE genes carried in serotypes Ib and V were highly associated with the resistance to macrolides and clindamycin. Mutations at codon 79 and codon 83 of ParC were the major determining factors for high-level fluoroquinolone resistance. |
| first_indexed | 2024-04-14T02:41:34Z |
| format | Article |
| id | doaj.art-a86bcbebb4ce4daabbf2f76c5352bfce |
| institution | Directory Open Access Journal |
| issn | 2319-4170 2320-2890 |
| language | English |
| last_indexed | 2024-04-14T02:41:34Z |
| publishDate | 2015-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biomedical Journal |
| spelling | doaj.art-a86bcbebb4ce4daabbf2f76c5352bfce2022-12-22T02:16:52ZengElsevierBiomedical Journal2319-41702320-28902015-06-0138321522010.4103/2319-4170.138306Serotype distribution and resistance genes associated with macrolide and fluoroquinolone resistance in Streptococcus agalactiae isolates from a hospital in southern TaiwanYing-Hsiang Wang Chyi-Liang Chen Jiun-Nub Hou Yi-Rou Wang Ting-Yu Lin Mei-Hei Wang Tsung-Han Yang Chishih ChuCheng-Hsun ChiuBackground: Antimicrobial resistance of Streptococcus agalactiae (Group B Streptococcus, GBS) has been emerging worldwide. We aimed to examine the correlation of drug-resistant genes with serotypes and with the mutations of the quinolone resistance-determining region (QRDR) in GBS isolates. Methods: A total of 323 human GBS isolates were collected from a hospital in southern Taiwan. Laboratory investigation included serotyping by a multiplex polymerase chain reaction (PCR) method, antimicrobial susceptibility testing by a disc diffusion method, and mechanism analysis of the resistance to macrolides and fluoroquinolones by PCR and sequencing methods. Results: Multiplex PCR showed that the most prevalent serotypes were Ib, III, V, and VI, mostly isolated from urine. The ermB gene was highly prevalent in serotypes Ib and V and was associated with clindamycin and macrolide resistance. GBS with a serine-to-leucine mutation at codon 81 in GyrA and with a serine-to-phenylalanine or -tyrosine mutation at codon 79 in ParC had a higher minimum inhibitory concentration of levofloxacin than isolates with only an aspartic acid-to-tyrosine mutation at codon 83 (>32 μg/ml vs. 16 μg/ml) in GyrA. Conclusions: The most prevalent GBS serotypes were Ib, III, V, and VI. The ermB and mefE genes carried in serotypes Ib and V were highly associated with the resistance to macrolides and clindamycin. Mutations at codon 79 and codon 83 of ParC were the major determining factors for high-level fluoroquinolone resistance.http://www.biomedj.org/article.asp?issn=2319-4170;year=2015;volume=38;issue=3;spage=215;epage=220;aulast=Wangantimicrobial resistancefluoroquinolonemacrolideserotypeStreptococcus agalactiae |
| spellingShingle | Ying-Hsiang Wang Chyi-Liang Chen Jiun-Nub Hou Yi-Rou Wang Ting-Yu Lin Mei-Hei Wang Tsung-Han Yang Chishih Chu Cheng-Hsun Chiu Serotype distribution and resistance genes associated with macrolide and fluoroquinolone resistance in Streptococcus agalactiae isolates from a hospital in southern Taiwan Biomedical Journal antimicrobial resistance fluoroquinolone macrolide serotype Streptococcus agalactiae |
| title | Serotype distribution and resistance genes associated with macrolide and fluoroquinolone resistance in Streptococcus agalactiae isolates from a hospital in southern Taiwan |
| title_full | Serotype distribution and resistance genes associated with macrolide and fluoroquinolone resistance in Streptococcus agalactiae isolates from a hospital in southern Taiwan |
| title_fullStr | Serotype distribution and resistance genes associated with macrolide and fluoroquinolone resistance in Streptococcus agalactiae isolates from a hospital in southern Taiwan |
| title_full_unstemmed | Serotype distribution and resistance genes associated with macrolide and fluoroquinolone resistance in Streptococcus agalactiae isolates from a hospital in southern Taiwan |
| title_short | Serotype distribution and resistance genes associated with macrolide and fluoroquinolone resistance in Streptococcus agalactiae isolates from a hospital in southern Taiwan |
| title_sort | serotype distribution and resistance genes associated with macrolide and fluoroquinolone resistance in streptococcus agalactiae isolates from a hospital in southern taiwan |
| topic | antimicrobial resistance fluoroquinolone macrolide serotype Streptococcus agalactiae |
| url | http://www.biomedj.org/article.asp?issn=2319-4170;year=2015;volume=38;issue=3;spage=215;epage=220;aulast=Wang |
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