Isolation and Identification of Hyaluronidase Producing Staphylococcus aureus

In this study, a total of one hundred clinical samples were collected for isolation of Staphylococcus aureus from different hospitals (Al Yarmouk Teaching Hospital and Hospital Child Central and a number of Al- Harthiya laboratories) in Baghdad. The diagnostic results showed that 34 out of 100 samp...

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Main Authors: Furqan M. K, Maysaa Ch. H, Asmaa A. H.
Format: Article
Language:English
Published: College of Pharmacy / Mustansiriyah University 2016-06-01
Series:Al-Mustansiriyah Journal of Pharmaceutical Sciences
Online Access:https://ajps.uomustansiriyah.edu.iq/index.php/AJPS/article/view/123
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author Furqan M. K
Maysaa Ch. H
Asmaa A. H.
author_facet Furqan M. K
Maysaa Ch. H
Asmaa A. H.
author_sort Furqan M. K
collection DOAJ
description In this study, a total of one hundred clinical samples were collected for isolation of Staphylococcus aureus from different hospitals (Al Yarmouk Teaching Hospital and Hospital Child Central and a number of Al- Harthiya laboratories) in Baghdad. The diagnostic results showed that 34 out of 100 samples were beloned to gram positive bacteria and 66 were gram negative according to their morphological and cultural characteristics. From these 34 gram positive isolates 25 isolates were confirmed as Staphylococcus aureus, 6 Staphylococcus epidermidis and 3 Staphylococcus saprophytic us. These S. aureus isolates were tested for their ability to produce hyaluronidase enzyme and results showed that all the isolates were hyaluronidase producers; among them, S. aureus isolate S2 obtained from urine was the most efficient in hyaluroniase production. Enzyme specific activity of the crude filtrate of this isolate was 96 U/mg proteins.
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spelling doaj.art-a86e24b4e5154174a62318c7898860352024-04-04T06:58:19ZengCollege of Pharmacy / Mustansiriyah UniversityAl-Mustansiriyah Journal of Pharmaceutical Sciences1815-09932959-183X2016-06-0114110.32947/ajps.v14i1.123Isolation and Identification of Hyaluronidase Producing Staphylococcus aureusFurqan M. K0Maysaa Ch. H1Asmaa A. H.2Department of Biotechnology, College of Science, University of Al-NahrainDepartment of Biotechnology, College of Science, University of Al-NahrainDepartment of Biotechnology, College of Science, University of Al-Nahrain In this study, a total of one hundred clinical samples were collected for isolation of Staphylococcus aureus from different hospitals (Al Yarmouk Teaching Hospital and Hospital Child Central and a number of Al- Harthiya laboratories) in Baghdad. The diagnostic results showed that 34 out of 100 samples were beloned to gram positive bacteria and 66 were gram negative according to their morphological and cultural characteristics. From these 34 gram positive isolates 25 isolates were confirmed as Staphylococcus aureus, 6 Staphylococcus epidermidis and 3 Staphylococcus saprophytic us. These S. aureus isolates were tested for their ability to produce hyaluronidase enzyme and results showed that all the isolates were hyaluronidase producers; among them, S. aureus isolate S2 obtained from urine was the most efficient in hyaluroniase production. Enzyme specific activity of the crude filtrate of this isolate was 96 U/mg proteins. https://ajps.uomustansiriyah.edu.iq/index.php/AJPS/article/view/123
spellingShingle Furqan M. K
Maysaa Ch. H
Asmaa A. H.
Isolation and Identification of Hyaluronidase Producing Staphylococcus aureus
Al-Mustansiriyah Journal of Pharmaceutical Sciences
title Isolation and Identification of Hyaluronidase Producing Staphylococcus aureus
title_full Isolation and Identification of Hyaluronidase Producing Staphylococcus aureus
title_fullStr Isolation and Identification of Hyaluronidase Producing Staphylococcus aureus
title_full_unstemmed Isolation and Identification of Hyaluronidase Producing Staphylococcus aureus
title_short Isolation and Identification of Hyaluronidase Producing Staphylococcus aureus
title_sort isolation and identification of hyaluronidase producing staphylococcus aureus
url https://ajps.uomustansiriyah.edu.iq/index.php/AJPS/article/view/123
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