Isolation and Identification of Hyaluronidase Producing Staphylococcus aureus
In this study, a total of one hundred clinical samples were collected for isolation of Staphylococcus aureus from different hospitals (Al Yarmouk Teaching Hospital and Hospital Child Central and a number of Al- Harthiya laboratories) in Baghdad. The diagnostic results showed that 34 out of 100 samp...
Main Authors: | , , |
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Format: | Article |
Language: | English |
Published: |
College of Pharmacy / Mustansiriyah University
2016-06-01
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Series: | Al-Mustansiriyah Journal of Pharmaceutical Sciences |
Online Access: | https://ajps.uomustansiriyah.edu.iq/index.php/AJPS/article/view/123 |
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author | Furqan M. K Maysaa Ch. H Asmaa A. H. |
author_facet | Furqan M. K Maysaa Ch. H Asmaa A. H. |
author_sort | Furqan M. K |
collection | DOAJ |
description |
In this study, a total of one hundred clinical samples were collected for isolation of
Staphylococcus aureus from different hospitals (Al Yarmouk Teaching Hospital and Hospital
Child Central and a number of Al- Harthiya laboratories) in Baghdad.
The diagnostic results showed that 34 out of 100 samples were beloned to gram positive bacteria and 66 were gram negative according to their morphological and cultural characteristics. From these 34 gram positive isolates 25 isolates were confirmed as Staphylococcus aureus, 6 Staphylococcus epidermidis and 3 Staphylococcus saprophytic us.
These S. aureus isolates were tested for their ability to produce hyaluronidase enzyme and results showed that all the isolates were hyaluronidase producers; among them, S. aureus isolate S2 obtained from urine was the most efficient in hyaluroniase production. Enzyme specific activity of the crude filtrate of this isolate was 96 U/mg proteins.
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first_indexed | 2024-04-24T13:45:15Z |
format | Article |
id | doaj.art-a86e24b4e5154174a62318c789886035 |
institution | Directory Open Access Journal |
issn | 1815-0993 2959-183X |
language | English |
last_indexed | 2024-04-24T13:45:15Z |
publishDate | 2016-06-01 |
publisher | College of Pharmacy / Mustansiriyah University |
record_format | Article |
series | Al-Mustansiriyah Journal of Pharmaceutical Sciences |
spelling | doaj.art-a86e24b4e5154174a62318c7898860352024-04-04T06:58:19ZengCollege of Pharmacy / Mustansiriyah UniversityAl-Mustansiriyah Journal of Pharmaceutical Sciences1815-09932959-183X2016-06-0114110.32947/ajps.v14i1.123Isolation and Identification of Hyaluronidase Producing Staphylococcus aureusFurqan M. K0Maysaa Ch. H1Asmaa A. H.2Department of Biotechnology, College of Science, University of Al-NahrainDepartment of Biotechnology, College of Science, University of Al-NahrainDepartment of Biotechnology, College of Science, University of Al-Nahrain In this study, a total of one hundred clinical samples were collected for isolation of Staphylococcus aureus from different hospitals (Al Yarmouk Teaching Hospital and Hospital Child Central and a number of Al- Harthiya laboratories) in Baghdad. The diagnostic results showed that 34 out of 100 samples were beloned to gram positive bacteria and 66 were gram negative according to their morphological and cultural characteristics. From these 34 gram positive isolates 25 isolates were confirmed as Staphylococcus aureus, 6 Staphylococcus epidermidis and 3 Staphylococcus saprophytic us. These S. aureus isolates were tested for their ability to produce hyaluronidase enzyme and results showed that all the isolates were hyaluronidase producers; among them, S. aureus isolate S2 obtained from urine was the most efficient in hyaluroniase production. Enzyme specific activity of the crude filtrate of this isolate was 96 U/mg proteins. https://ajps.uomustansiriyah.edu.iq/index.php/AJPS/article/view/123 |
spellingShingle | Furqan M. K Maysaa Ch. H Asmaa A. H. Isolation and Identification of Hyaluronidase Producing Staphylococcus aureus Al-Mustansiriyah Journal of Pharmaceutical Sciences |
title | Isolation and Identification of Hyaluronidase Producing Staphylococcus aureus |
title_full | Isolation and Identification of Hyaluronidase Producing Staphylococcus aureus |
title_fullStr | Isolation and Identification of Hyaluronidase Producing Staphylococcus aureus |
title_full_unstemmed | Isolation and Identification of Hyaluronidase Producing Staphylococcus aureus |
title_short | Isolation and Identification of Hyaluronidase Producing Staphylococcus aureus |
title_sort | isolation and identification of hyaluronidase producing staphylococcus aureus |
url | https://ajps.uomustansiriyah.edu.iq/index.php/AJPS/article/view/123 |
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