Nuphar lutea Extracts Exhibit Anti-Viral Activity against the Measles Virus
Different parts of <i>Nuphar lutea L</i>. (yellow water lily) have been used to treat several inflammatory and pathogen-related diseases. It has shown that <i>Nuphar lutea</i> extracts (NUP) are active against various pathogens including bacteria, fungi, and leishmanial paras...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-04-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/25/7/1657 |
| _version_ | 1797571478316646400 |
|---|---|
| author | Hila Winer Janet Ozer Yonat Shemer Irit Reichenstein Brit Eilam-Frenkel Daniel Benharroch Avi Golan-Goldhirsh Jacob Gopas |
| author_facet | Hila Winer Janet Ozer Yonat Shemer Irit Reichenstein Brit Eilam-Frenkel Daniel Benharroch Avi Golan-Goldhirsh Jacob Gopas |
| author_sort | Hila Winer |
| collection | DOAJ |
| description | Different parts of <i>Nuphar lutea L</i>. (yellow water lily) have been used to treat several inflammatory and pathogen-related diseases. It has shown that <i>Nuphar lutea</i> extracts (NUP) are active against various pathogens including bacteria, fungi, and leishmanial parasites. In an effort to detect novel therapeutic agents against negative-stranded RNA (- RNA) viruses, we have tested the effect of a partially-purified alkaloid mixture of Nuphar lutea leaves on the measles virus (MV). The MV vaccine’s Edmonston strain was used to acutely or persistently infect cells. The levels of several MV proteins were detected by a Western blot and immunocytochemistry. Viral RNAs were quantitated by qRT-PCR. Virus infectivity was monitored by infecting African green monkey kidney VERO cells’ monolayers. We showed that NUP protected cells from acute infection. Decreases in the MV P-, N-, and V-proteins were observed in persistently infected cells and the amount of infective virus released was reduced as compared to untreated cells. By examining viral RNAs, we suggest that NUP acts at the post-transcriptional level. We conclude, as a proof of concept, that NUP has anti-viral therapeutic activity against the MV. Future studies will determine the mechanism of action and the effect of NUP on other related viruses. |
| first_indexed | 2024-03-10T20:41:14Z |
| format | Article |
| id | doaj.art-a8715a8ad95a4b5685bb9798448166f0 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T20:41:14Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-a8715a8ad95a4b5685bb9798448166f02023-11-19T20:39:44ZengMDPI AGMolecules1420-30492020-04-01257165710.3390/molecules25071657Nuphar lutea Extracts Exhibit Anti-Viral Activity against the Measles VirusHila Winer0Janet Ozer1Yonat Shemer2Irit Reichenstein3Brit Eilam-Frenkel4Daniel Benharroch5Avi Golan-Goldhirsh6Jacob Gopas7Department of Microbiology and Immunology and Genetics, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer Sheva 8400501, IsraelDepartment of Microbiology and Immunology and Genetics, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer Sheva 8400501, IsraelDepartment of Microbiology and Immunology and Genetics, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer Sheva 8400501, IsraelDepartment of Microbiology and Immunology and Genetics, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer Sheva 8400501, IsraelDepartment of Microbiology and Immunology and Genetics, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer Sheva 8400501, IsraelDepartment of Pathology, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer Sheva 8400501, IsraelThe Jacob Blaustein Institutes for Desert Research; Albert Katz Department of Dryland Biotechnologies, Sede Boqer, Ben-Gurion University of the Negev, Beer Sheva 8400501, IsraelDepartment of Microbiology and Immunology and Genetics, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer Sheva 8400501, IsraelDifferent parts of <i>Nuphar lutea L</i>. (yellow water lily) have been used to treat several inflammatory and pathogen-related diseases. It has shown that <i>Nuphar lutea</i> extracts (NUP) are active against various pathogens including bacteria, fungi, and leishmanial parasites. In an effort to detect novel therapeutic agents against negative-stranded RNA (- RNA) viruses, we have tested the effect of a partially-purified alkaloid mixture of Nuphar lutea leaves on the measles virus (MV). The MV vaccine’s Edmonston strain was used to acutely or persistently infect cells. The levels of several MV proteins were detected by a Western blot and immunocytochemistry. Viral RNAs were quantitated by qRT-PCR. Virus infectivity was monitored by infecting African green monkey kidney VERO cells’ monolayers. We showed that NUP protected cells from acute infection. Decreases in the MV P-, N-, and V-proteins were observed in persistently infected cells and the amount of infective virus released was reduced as compared to untreated cells. By examining viral RNAs, we suggest that NUP acts at the post-transcriptional level. We conclude, as a proof of concept, that NUP has anti-viral therapeutic activity against the MV. Future studies will determine the mechanism of action and the effect of NUP on other related viruses.https://www.mdpi.com/1420-3049/25/7/1657herbal medicines<i>Nuphar lutea</i> plant extractnupharidinesmeasles virusanti-viral natural products |
| spellingShingle | Hila Winer Janet Ozer Yonat Shemer Irit Reichenstein Brit Eilam-Frenkel Daniel Benharroch Avi Golan-Goldhirsh Jacob Gopas Nuphar lutea Extracts Exhibit Anti-Viral Activity against the Measles Virus Molecules herbal medicines <i>Nuphar lutea</i> plant extract nupharidines measles virus anti-viral natural products |
| title | Nuphar lutea Extracts Exhibit Anti-Viral Activity against the Measles Virus |
| title_full | Nuphar lutea Extracts Exhibit Anti-Viral Activity against the Measles Virus |
| title_fullStr | Nuphar lutea Extracts Exhibit Anti-Viral Activity against the Measles Virus |
| title_full_unstemmed | Nuphar lutea Extracts Exhibit Anti-Viral Activity against the Measles Virus |
| title_short | Nuphar lutea Extracts Exhibit Anti-Viral Activity against the Measles Virus |
| title_sort | nuphar lutea extracts exhibit anti viral activity against the measles virus |
| topic | herbal medicines <i>Nuphar lutea</i> plant extract nupharidines measles virus anti-viral natural products |
| url | https://www.mdpi.com/1420-3049/25/7/1657 |
| work_keys_str_mv | AT hilawiner nupharluteaextractsexhibitantiviralactivityagainstthemeaslesvirus AT janetozer nupharluteaextractsexhibitantiviralactivityagainstthemeaslesvirus AT yonatshemer nupharluteaextractsexhibitantiviralactivityagainstthemeaslesvirus AT iritreichenstein nupharluteaextractsexhibitantiviralactivityagainstthemeaslesvirus AT briteilamfrenkel nupharluteaextractsexhibitantiviralactivityagainstthemeaslesvirus AT danielbenharroch nupharluteaextractsexhibitantiviralactivityagainstthemeaslesvirus AT avigolangoldhirsh nupharluteaextractsexhibitantiviralactivityagainstthemeaslesvirus AT jacobgopas nupharluteaextractsexhibitantiviralactivityagainstthemeaslesvirus |