Difference in composition and functional analysis of bacterial communities between Mytilus galloprovincialis gills and surrounding water in a brackish inshore bay, analyzed by 16S rDNA multi-amplicon sequencing
Abstract Background Lagoons are important natural systems, with attractive favorable conditions for aquaculture production, such as shellfish cultivation. Despite their socio-economic relevance for human activity, information on the microbial diversity, community composition, and putative functions...
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| Format: | Article |
| Language: | English |
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BMC
2024-01-01
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| Series: | Annals of Microbiology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13213-024-01749-8 |
| _version_ | 1797350221437468672 |
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| author | Giulia Zardinoni Saptarathi Deb Samathmika Ravi Lucia Giagnoni Piergiorgio Fumelli Alessandra Tondello Mauro Dacasto Andrea Squartini Carlo Spanu Angela Trocino Piergiorgio Stevanato |
| author_facet | Giulia Zardinoni Saptarathi Deb Samathmika Ravi Lucia Giagnoni Piergiorgio Fumelli Alessandra Tondello Mauro Dacasto Andrea Squartini Carlo Spanu Angela Trocino Piergiorgio Stevanato |
| author_sort | Giulia Zardinoni |
| collection | DOAJ |
| description | Abstract Background Lagoons are important natural systems, with attractive favorable conditions for aquaculture production, such as shellfish cultivation. Despite their socio-economic relevance for human activity, information on the microbial diversity, community composition, and putative functions of gill-associated microbiota and seawater is still limited, particularly as regards the extent of specific taxa enrichment within the gills and the relative effects of the temporal and spatial variables. In this study, we used a 16S rDNA multi-amplicon sequencing approach using an Ion GeneStudio S5 System and a function prediction method (Functional Annotation of Prokaryotic Taxa (FAPROTAX), to inspect the springtime dynamics of microbial communities and their inferred metabolic features in an Adriatic lagoon (Po Delta, Italy). Results Mussels and surrounding seawater were sampled in two rearing areas three times between April and June 2021. Sequencing results showed significant (p ≤ 0.05) differences in bacterial community composition and diversity between gills and seawater. Gills were dominated by the Methylobacterium-Methylorubrum and Burkholderia-Caballeronia-Paraburkholderia genera, while in seawater samples Izamaplasma, Planktomarina, and Candidatus Aquiluna were detected as being dominant. The microbiota composition did not differ significantly between the two rearing areas. The sampling time, although limited to a 3-month timeframe, instead revealed a structural variation of the bacterial profile both in gills and seawater for alpha and beta diversities respectively. The functional prediction analysis highlighted an overexpression of human gut-associated bacteria in relation to the season-related increase in seawater temperature. Conclusions These findings enhance our understanding of the differences between gill-associated and seawater microbiota composition and provide novel insights into the functions carried out by bacteria inhabiting these niches, as well as on the key host-symbiont relationships of bivalves in lagoon environments. |
| first_indexed | 2024-03-08T12:41:33Z |
| format | Article |
| id | doaj.art-a8729340c145471b976b6b2629c0494c |
| institution | Directory Open Access Journal |
| issn | 1869-2044 |
| language | English |
| last_indexed | 2024-03-08T12:41:33Z |
| publishDate | 2024-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Annals of Microbiology |
| spelling | doaj.art-a8729340c145471b976b6b2629c0494c2024-01-21T12:07:23ZengBMCAnnals of Microbiology1869-20442024-01-0174111510.1186/s13213-024-01749-8Difference in composition and functional analysis of bacterial communities between Mytilus galloprovincialis gills and surrounding water in a brackish inshore bay, analyzed by 16S rDNA multi-amplicon sequencingGiulia Zardinoni0Saptarathi Deb1Samathmika Ravi2Lucia Giagnoni3Piergiorgio Fumelli4Alessandra Tondello5Mauro Dacasto6Andrea Squartini7Carlo Spanu8Angela Trocino9Piergiorgio Stevanato10Department of Agronomy, Food, Natural Resources, Animals and Environment (DAFNAE), University of Padova, Viale dell’Università 16Department of Agronomy, Food, Natural Resources, Animals and Environment (DAFNAE), University of Padova, Viale dell’Università 16Department of Agronomy, Food, Natural Resources, Animals and Environment (DAFNAE), University of Padova, Viale dell’Università 16Department of Veterinary Medicine, University of Sassari,Via Vienna 2Department. of Prevention, Servizio Veterinario A.U.L.S.S. 5 POLESANADepartment of Agronomy, Food, Natural Resources, Animals and Environment (DAFNAE), University of Padova, Viale dell’Università 16Department of Comparative Biomedicine and Food Science (BCA), University of Padova, Viale dell’Università 16Department of Agronomy, Food, Natural Resources, Animals and Environment (DAFNAE), University of Padova, Viale dell’Università 16Department of Veterinary Medicine, University of Sassari,Via Vienna 2Department of Agronomy, Food, Natural Resources, Animals and Environment (DAFNAE), University of Padova, Viale dell’Università 16Department of Agronomy, Food, Natural Resources, Animals and Environment (DAFNAE), University of Padova, Viale dell’Università 16Abstract Background Lagoons are important natural systems, with attractive favorable conditions for aquaculture production, such as shellfish cultivation. Despite their socio-economic relevance for human activity, information on the microbial diversity, community composition, and putative functions of gill-associated microbiota and seawater is still limited, particularly as regards the extent of specific taxa enrichment within the gills and the relative effects of the temporal and spatial variables. In this study, we used a 16S rDNA multi-amplicon sequencing approach using an Ion GeneStudio S5 System and a function prediction method (Functional Annotation of Prokaryotic Taxa (FAPROTAX), to inspect the springtime dynamics of microbial communities and their inferred metabolic features in an Adriatic lagoon (Po Delta, Italy). Results Mussels and surrounding seawater were sampled in two rearing areas three times between April and June 2021. Sequencing results showed significant (p ≤ 0.05) differences in bacterial community composition and diversity between gills and seawater. Gills were dominated by the Methylobacterium-Methylorubrum and Burkholderia-Caballeronia-Paraburkholderia genera, while in seawater samples Izamaplasma, Planktomarina, and Candidatus Aquiluna were detected as being dominant. The microbiota composition did not differ significantly between the two rearing areas. The sampling time, although limited to a 3-month timeframe, instead revealed a structural variation of the bacterial profile both in gills and seawater for alpha and beta diversities respectively. The functional prediction analysis highlighted an overexpression of human gut-associated bacteria in relation to the season-related increase in seawater temperature. Conclusions These findings enhance our understanding of the differences between gill-associated and seawater microbiota composition and provide novel insights into the functions carried out by bacteria inhabiting these niches, as well as on the key host-symbiont relationships of bivalves in lagoon environments.https://doi.org/10.1186/s13213-024-01749-8GillsMussels16s rDNA amplicon sequencingBacterial profileMicrobiota-environment interactions |
| spellingShingle | Giulia Zardinoni Saptarathi Deb Samathmika Ravi Lucia Giagnoni Piergiorgio Fumelli Alessandra Tondello Mauro Dacasto Andrea Squartini Carlo Spanu Angela Trocino Piergiorgio Stevanato Difference in composition and functional analysis of bacterial communities between Mytilus galloprovincialis gills and surrounding water in a brackish inshore bay, analyzed by 16S rDNA multi-amplicon sequencing Annals of Microbiology Gills Mussels 16s rDNA amplicon sequencing Bacterial profile Microbiota-environment interactions |
| title | Difference in composition and functional analysis of bacterial communities between Mytilus galloprovincialis gills and surrounding water in a brackish inshore bay, analyzed by 16S rDNA multi-amplicon sequencing |
| title_full | Difference in composition and functional analysis of bacterial communities between Mytilus galloprovincialis gills and surrounding water in a brackish inshore bay, analyzed by 16S rDNA multi-amplicon sequencing |
| title_fullStr | Difference in composition and functional analysis of bacterial communities between Mytilus galloprovincialis gills and surrounding water in a brackish inshore bay, analyzed by 16S rDNA multi-amplicon sequencing |
| title_full_unstemmed | Difference in composition and functional analysis of bacterial communities between Mytilus galloprovincialis gills and surrounding water in a brackish inshore bay, analyzed by 16S rDNA multi-amplicon sequencing |
| title_short | Difference in composition and functional analysis of bacterial communities between Mytilus galloprovincialis gills and surrounding water in a brackish inshore bay, analyzed by 16S rDNA multi-amplicon sequencing |
| title_sort | difference in composition and functional analysis of bacterial communities between mytilus galloprovincialis gills and surrounding water in a brackish inshore bay analyzed by 16s rdna multi amplicon sequencing |
| topic | Gills Mussels 16s rDNA amplicon sequencing Bacterial profile Microbiota-environment interactions |
| url | https://doi.org/10.1186/s13213-024-01749-8 |
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