Selenium-binding protein 1 (SELENBP1) is a marker of mature adipocytes
Selenium-binding protein 1 (SELENBP1) has recently been reported to catalyse the oxidation of methanethiol, an organosulfur compound produced by gut microbiota. Two of the reaction products of methanethiol oxidation, hydrogen peroxide and hydrogen sulphide, serve as signalling molecules for cell dif...
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| Format: | Article |
| Language: | English |
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Elsevier
2019-01-01
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| Series: | Redox Biology |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S221323171830870X |
| _version_ | 1811195084019859456 |
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| author | Holger Steinbrenner Mustafa Micoogullari Ngoc Anh Hoang Ina Bergheim Lars-Oliver Klotz Helmut Sies |
| author_facet | Holger Steinbrenner Mustafa Micoogullari Ngoc Anh Hoang Ina Bergheim Lars-Oliver Klotz Helmut Sies |
| author_sort | Holger Steinbrenner |
| collection | DOAJ |
| description | Selenium-binding protein 1 (SELENBP1) has recently been reported to catalyse the oxidation of methanethiol, an organosulfur compound produced by gut microbiota. Two of the reaction products of methanethiol oxidation, hydrogen peroxide and hydrogen sulphide, serve as signalling molecules for cell differentiation. Indeed, colonocyte differentiation has been found to be associated with SELENBP1 induction. Here, we show that SELENBP1 is induced when 3T3-L1 preadipocytes undergo terminal differentiation and maturation to adipocytes. SELENBP1 induction succeeded the up-regulation of known marker proteins of white adipocytes and the intracellular accumulation of lipids. Immunofluorescence microscopy revealed predominant cytoplasmic localisation of SELENBP1 in 3T3-L1 adipocytes, as demonstrated by co-staining with the key lipogenic enzyme, acetyl-CoA-carboxylase (ACC), located in cytosol. In differentiating 3T3-L1 cells, the mTOR inhibitor rapamycin and the pro-inflammatory cytokine tumour necrosis factor alpha (TNF-α) likewise suppressed SELENBP1 induction, adipocyte differentiation and lipid accumulation. However, lipid accumulation per se is not linked to SELENBP1 induction, as hepatic SELENBP1 was down-regulated in high fructose-fed mice despite increased lipogenesis in the liver and development of non-alcoholic fatty liver disease (NAFLD). In conclusion, SELENBP1 is a marker of cell differentiation/maturation rather than being linked to lipogenesis/lipid accumulation. Keywords: 3T3-L1, Adipogenesis, Lipid accumulation, NAFLD, Selenium, GPx1 |
| first_indexed | 2024-04-12T00:36:37Z |
| format | Article |
| id | doaj.art-a8737f910c754d2999fcc29fe0aa2623 |
| institution | Directory Open Access Journal |
| issn | 2213-2317 |
| language | English |
| last_indexed | 2024-04-12T00:36:37Z |
| publishDate | 2019-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Redox Biology |
| spelling | doaj.art-a8737f910c754d2999fcc29fe0aa26232022-12-22T03:55:08ZengElsevierRedox Biology2213-23172019-01-0120489495Selenium-binding protein 1 (SELENBP1) is a marker of mature adipocytesHolger Steinbrenner0Mustafa Micoogullari1Ngoc Anh Hoang2Ina Bergheim3Lars-Oliver Klotz4Helmut Sies5Institute of Nutritional Sciences, Nutrigenomics, Friedrich Schiller University Jena, Jena, Germany; Correspondence to: Institute of Nutritional Sciences, Nutrigenomics, Friedrich Schiller University Jena, Dornburger Strasse 29, D-07743 Jena, Germany.Institute of Biochemistry and Molecular Biology I, Heinrich Heine University Düsseldorf, Düsseldorf, GermanyInstitute of Nutritional Sciences, Nutrigenomics, Friedrich Schiller University Jena, Jena, GermanyDepartment of Nutritional Sciences, Molecular Nutritional Science, University Vienna, Vienna, AustriaInstitute of Nutritional Sciences, Nutrigenomics, Friedrich Schiller University Jena, Jena, GermanyInstitute of Biochemistry and Molecular Biology I, Heinrich Heine University Düsseldorf, Düsseldorf, Germany; Leibniz Research Institute for Environmental Medicine, Düsseldorf, GermanySelenium-binding protein 1 (SELENBP1) has recently been reported to catalyse the oxidation of methanethiol, an organosulfur compound produced by gut microbiota. Two of the reaction products of methanethiol oxidation, hydrogen peroxide and hydrogen sulphide, serve as signalling molecules for cell differentiation. Indeed, colonocyte differentiation has been found to be associated with SELENBP1 induction. Here, we show that SELENBP1 is induced when 3T3-L1 preadipocytes undergo terminal differentiation and maturation to adipocytes. SELENBP1 induction succeeded the up-regulation of known marker proteins of white adipocytes and the intracellular accumulation of lipids. Immunofluorescence microscopy revealed predominant cytoplasmic localisation of SELENBP1 in 3T3-L1 adipocytes, as demonstrated by co-staining with the key lipogenic enzyme, acetyl-CoA-carboxylase (ACC), located in cytosol. In differentiating 3T3-L1 cells, the mTOR inhibitor rapamycin and the pro-inflammatory cytokine tumour necrosis factor alpha (TNF-α) likewise suppressed SELENBP1 induction, adipocyte differentiation and lipid accumulation. However, lipid accumulation per se is not linked to SELENBP1 induction, as hepatic SELENBP1 was down-regulated in high fructose-fed mice despite increased lipogenesis in the liver and development of non-alcoholic fatty liver disease (NAFLD). In conclusion, SELENBP1 is a marker of cell differentiation/maturation rather than being linked to lipogenesis/lipid accumulation. Keywords: 3T3-L1, Adipogenesis, Lipid accumulation, NAFLD, Selenium, GPx1http://www.sciencedirect.com/science/article/pii/S221323171830870X |
| spellingShingle | Holger Steinbrenner Mustafa Micoogullari Ngoc Anh Hoang Ina Bergheim Lars-Oliver Klotz Helmut Sies Selenium-binding protein 1 (SELENBP1) is a marker of mature adipocytes Redox Biology |
| title | Selenium-binding protein 1 (SELENBP1) is a marker of mature adipocytes |
| title_full | Selenium-binding protein 1 (SELENBP1) is a marker of mature adipocytes |
| title_fullStr | Selenium-binding protein 1 (SELENBP1) is a marker of mature adipocytes |
| title_full_unstemmed | Selenium-binding protein 1 (SELENBP1) is a marker of mature adipocytes |
| title_short | Selenium-binding protein 1 (SELENBP1) is a marker of mature adipocytes |
| title_sort | selenium binding protein 1 selenbp1 is a marker of mature adipocytes |
| url | http://www.sciencedirect.com/science/article/pii/S221323171830870X |
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