Utilisation of Chick Embryo Chorioallantoic Membrane as a Model Platform for Imaging-Navigated Biomedical Research
The fertilised chick egg and particularly its chorioallantoic membrane (CAM) have drawn continuing interest in biomedicine and bioengineering fields, especially for research on vascular study, cancer, drug screening and development, cell factors, stem cells, etc. This literature review systemically...
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MDPI AG
2021-02-01
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| Online Access: | https://www.mdpi.com/2073-4409/10/2/463 |
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| author | Lei Chen Shuncong Wang Yuanbo Feng Jinyong Zhang Yuqing Du Jiang Zhang Chantal Van Ongeval Yicheng Ni Yue Li |
| author_facet | Lei Chen Shuncong Wang Yuanbo Feng Jinyong Zhang Yuqing Du Jiang Zhang Chantal Van Ongeval Yicheng Ni Yue Li |
| author_sort | Lei Chen |
| collection | DOAJ |
| description | The fertilised chick egg and particularly its chorioallantoic membrane (CAM) have drawn continuing interest in biomedicine and bioengineering fields, especially for research on vascular study, cancer, drug screening and development, cell factors, stem cells, etc. This literature review systemically introduces the CAM’s structural evolution, functions, vascular features and the circulation system, and cell regulatory factors. It also presents the major and updated applications of the CAM in assays for pharmacokinetics and biodistribution, drug efficacy and toxicology testing/screening in preclinical pharmacological research. The time course of CAM applications for different assays and their advantages and limitations are summarised. Among these applications, two aspects are emphasised: (1) potential utility of the CAM for preclinical studies on vascular-disrupting agents (VDAs), promising for anti-cancer vascular-targeted therapy, and (2) modern imaging technologies, including modalities and their applications for real-time visualisation, monitoring and evaluation of the changes in CAM vasculature as well as the interactions occurring after introducing the tested medical, pharmaceutical and biological agents into the system. The aim of this article is to help those working in the biomedical field to familiarise themselves with the chick embryo CAM as an alternative platform and to utilise it to design and optimise experimental settings for their specific research topics. |
| first_indexed | 2024-03-09T00:39:45Z |
| format | Article |
| id | doaj.art-a875fad883f74e6c9c23b58b2b6eef35 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-09T00:39:45Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-a875fad883f74e6c9c23b58b2b6eef352023-12-11T17:56:39ZengMDPI AGCells2073-44092021-02-0110246310.3390/cells10020463Utilisation of Chick Embryo Chorioallantoic Membrane as a Model Platform for Imaging-Navigated Biomedical ResearchLei Chen0Shuncong Wang1Yuanbo Feng2Jinyong Zhang3Yuqing Du4Jiang Zhang5Chantal Van Ongeval6Yicheng Ni7Yue Li8KU Leuven, Biomedical Group, Campus Gasthuisberg, 3000 Leuven, BelgiumKU Leuven, Biomedical Group, Campus Gasthuisberg, 3000 Leuven, BelgiumKU Leuven, Biomedical Group, Campus Gasthuisberg, 3000 Leuven, BelgiumShanghai Key Laboratory of Molecular Imaging, Shanghai University of Medicine and Health Sciences, Shanghai 201318, ChinaShanghai Key Laboratory of Molecular Imaging, Shanghai University of Medicine and Health Sciences, Shanghai 201318, ChinaFaculty of Agricultural Biotechnology and Ecotechnology, Shanghai Vocational College of Agriculture and Forestry, Shanghai 201600, ChinaKU Leuven, Biomedical Group, Campus Gasthuisberg, 3000 Leuven, BelgiumKU Leuven, Biomedical Group, Campus Gasthuisberg, 3000 Leuven, BelgiumShanghai Key Laboratory of Molecular Imaging, Shanghai University of Medicine and Health Sciences, Shanghai 201318, ChinaThe fertilised chick egg and particularly its chorioallantoic membrane (CAM) have drawn continuing interest in biomedicine and bioengineering fields, especially for research on vascular study, cancer, drug screening and development, cell factors, stem cells, etc. This literature review systemically introduces the CAM’s structural evolution, functions, vascular features and the circulation system, and cell regulatory factors. It also presents the major and updated applications of the CAM in assays for pharmacokinetics and biodistribution, drug efficacy and toxicology testing/screening in preclinical pharmacological research. The time course of CAM applications for different assays and their advantages and limitations are summarised. Among these applications, two aspects are emphasised: (1) potential utility of the CAM for preclinical studies on vascular-disrupting agents (VDAs), promising for anti-cancer vascular-targeted therapy, and (2) modern imaging technologies, including modalities and their applications for real-time visualisation, monitoring and evaluation of the changes in CAM vasculature as well as the interactions occurring after introducing the tested medical, pharmaceutical and biological agents into the system. The aim of this article is to help those working in the biomedical field to familiarise themselves with the chick embryo CAM as an alternative platform and to utilise it to design and optimise experimental settings for their specific research topics.https://www.mdpi.com/2073-4409/10/2/463chick embryochorioallantoic membrane (CAM)preclinical pharmacological researchvascular disrupting agents (VDAs)imaging techniques |
| spellingShingle | Lei Chen Shuncong Wang Yuanbo Feng Jinyong Zhang Yuqing Du Jiang Zhang Chantal Van Ongeval Yicheng Ni Yue Li Utilisation of Chick Embryo Chorioallantoic Membrane as a Model Platform for Imaging-Navigated Biomedical Research Cells chick embryo chorioallantoic membrane (CAM) preclinical pharmacological research vascular disrupting agents (VDAs) imaging techniques |
| title | Utilisation of Chick Embryo Chorioallantoic Membrane as a Model Platform for Imaging-Navigated Biomedical Research |
| title_full | Utilisation of Chick Embryo Chorioallantoic Membrane as a Model Platform for Imaging-Navigated Biomedical Research |
| title_fullStr | Utilisation of Chick Embryo Chorioallantoic Membrane as a Model Platform for Imaging-Navigated Biomedical Research |
| title_full_unstemmed | Utilisation of Chick Embryo Chorioallantoic Membrane as a Model Platform for Imaging-Navigated Biomedical Research |
| title_short | Utilisation of Chick Embryo Chorioallantoic Membrane as a Model Platform for Imaging-Navigated Biomedical Research |
| title_sort | utilisation of chick embryo chorioallantoic membrane as a model platform for imaging navigated biomedical research |
| topic | chick embryo chorioallantoic membrane (CAM) preclinical pharmacological research vascular disrupting agents (VDAs) imaging techniques |
| url | https://www.mdpi.com/2073-4409/10/2/463 |
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